دورية أكاديمية
Molecular recording of calcium signals via calcium-dependent proximity labeling.
| العنوان: | Molecular recording of calcium signals via calcium-dependent proximity labeling. |
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| المؤلفون: | Kim JW; Department of Molecular and Cell Biology at the University of California, Berkeley, Berkeley, CA, USA., Yong AJH; Department of Physiology at the University of California, San Francisco, San Francisco, CA, USA.; Howard Hughes Medical Institute at the University of California, San Francisco, San Francisco, CA, USA., Aisenberg EE; Helen Wills Neuroscience Institute at the University of California, Berkeley, Berkeley, CA, USA., Lobel JH; Department of Molecular and Cell Biology at the University of California, Berkeley, Berkeley, CA, USA., Wang W; Department of Chemistry at the University of Illinois, Chicago, Chicago, IL, USA., Dawson TM; Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Dawson VL; Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Gao R; Department of Chemistry at the University of Illinois, Chicago, Chicago, IL, USA., Jan YN; Department of Physiology at the University of California, San Francisco, San Francisco, CA, USA.; Howard Hughes Medical Institute at the University of California, San Francisco, San Francisco, CA, USA., Bateup HS; Department of Molecular and Cell Biology at the University of California, Berkeley, Berkeley, CA, USA.; Helen Wills Neuroscience Institute at the University of California, Berkeley, Berkeley, CA, USA.; Chan Zuckerberg Biohub, San Francisco, CA, USA., Ingolia NT; Department of Molecular and Cell Biology at the University of California, Berkeley, Berkeley, CA, USA. ingolia@berkeley.edu. |
| المصدر: | Nature chemical biology [Nat Chem Biol] 2024 Jul; Vol. 20 (7), pp. 894-905. Date of Electronic Publication: 2024 Apr 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101231976 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4469 (Electronic) Linking ISSN: 15524450 NLM ISO Abbreviation: Nat Chem Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Nature Pub. Group, [2005]- |
| مواضيع طبية MeSH: | Calcium*/metabolism , Neurons*/metabolism , Calcium Signaling* , Biotinylation*, Animals ; Carbon-Nitrogen Ligases/metabolism ; Carbon-Nitrogen Ligases/chemistry ; Humans ; Mice ; HEK293 Cells ; Repressor Proteins ; Escherichia coli Proteins |
| مستخلص: | Calcium ions serve as key intracellular signals. Local, transient increases in calcium concentrations can activate calcium sensor proteins that in turn trigger downstream effectors. In neurons, calcium transients play a central role in regulating neurotransmitter release and synaptic plasticity. However, it is challenging to capture the molecular events associated with these localized and ephemeral calcium signals. Here we present an engineered biotin ligase that generates permanent molecular traces in a calcium-dependent manner. The enzyme, calcium-dependent BioID (Cal-ID), biotinylates nearby proteins within minutes in response to elevated local calcium levels. The biotinylated proteins can be identified via mass spectrometry and visualized using microscopy. In neurons, Cal-ID labeling is triggered by neuronal activity, leading to prominent protein biotinylation that enables transcription-independent activity labeling in the brain. In summary, Cal-ID produces a biochemical record of calcium signals and neuronal activity with high spatial resolution and molecular specificity. (© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.) |
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| معلومات مُعتمدة: | DP2CA195768 U.S. Department of Health & Human Services | NIH | NIH Office of the Director (OD); R21NS112842 U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS); R35NS097227 U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) |
| المشرفين على المادة: | SY7Q814VUP (Calcium) EC 6.3.- (Carbon-Nitrogen Ligases) EC 6.3.4.15 (birA protein, E coli) 0 (Repressor Proteins) 0 (Escherichia coli Proteins) |
| تواريخ الأحداث: | Date Created: 20240424 Date Completed: 20240628 Latest Revision: 20240628 |
| رمز التحديث: | 20240629 |
| DOI: | 10.1038/s41589-024-01603-7 |
| PMID: | 38658655 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1552-4469 |
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| DOI: | 10.1038/s41589-024-01603-7 |