دورية أكاديمية
أعرض في EDS

Sunken Riches: Ascomycete Diversity in the Western Mediterranean Coast through Direct Plating and Flocculation, and Description of Four New Taxa.

التفاصيل البيبلوغرافية
العنوان: Sunken Riches: Ascomycete Diversity in the Western Mediterranean Coast through Direct Plating and Flocculation, and Description of Four New Taxa.
المؤلفون: Guerra-Mateo D; Unitat de Micologia i Microbiologia Ambiental, Facultat de Medicina i Ciències de la Salut and Institut Universitari de Recerca en Sostenibilitat, Canvi Climàtic i Transició Energètica (IU-RESCAT), Universitat Rovira i Virgili, 43201 Reus, Spain., Cano-Lira JF; Unitat de Micologia i Microbiologia Ambiental, Facultat de Medicina i Ciències de la Salut and Institut Universitari de Recerca en Sostenibilitat, Canvi Climàtic i Transició Energètica (IU-RESCAT), Universitat Rovira i Virgili, 43201 Reus, Spain., Fernández-Bravo A; Unitat de Micologia i Microbiologia Ambiental, Facultat de Medicina i Ciències de la Salut and Institut Universitari de Recerca en Sostenibilitat, Canvi Climàtic i Transició Energètica (IU-RESCAT), Universitat Rovira i Virgili, 43201 Reus, Spain., Gené J; Unitat de Micologia i Microbiologia Ambiental, Facultat de Medicina i Ciències de la Salut and Institut Universitari de Recerca en Sostenibilitat, Canvi Climàtic i Transició Energètica (IU-RESCAT), Universitat Rovira i Virgili, 43201 Reus, Spain.
المصدر: Journal of fungi (Basel, Switzerland) [J Fungi (Basel)] 2024 Apr 11; Vol. 10 (4). Date of Electronic Publication: 2024 Apr 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101671827 Publication Model: Electronic Cited Medium: Internet ISSN: 2309-608X (Electronic) Linking ISSN: 2309608X NLM ISO Abbreviation: J Fungi (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2015]-
مستخلص: The Mediterranean Sea stands out as a hotspot of biodiversity, whose fungal composition remains underexplored. Marine sediments represent the most diverse substrate; however, the challenge of recovering fungi in culture hinders the precise identification of this diversity. Concentration techniques like skimmed milk flocculation (SMF) could represent a suitable solution. Here, we compare the effectiveness in recovering filamentous ascomycetes of direct plating and SMF in combination with three culture media and two incubation temperatures, and we describe the fungal diversity detected in marine sediments. Sediments were collected at different depths on two beaches (Miracle and Arrabassada) on the Spanish western Mediterranean coast between 2021 and 2022. We recovered 362 strains, and after a morphological selection, 188 were identified primarily with the LSU and ITS barcodes, representing 54 genera and 94 species. Aspergillus , Penicillium , and Scedosporium were the most common genera, with different percentages of abundance between both beaches. Arrabassada Beach was more heterogeneous, with 42 genera representing 60 species (Miracle Beach, 28 genera and 54 species). Although most species were recovered with direct plating (70 species), 20 species were exclusively obtained using SMF as a sample pre-treatment, improving our ability to detect fungi in culture. In addition, we propose three new species in the genera Exophiala , Nigrocephalum , and Queenslandipenidiella , and a fourth representing the novel genus Schizochlamydosporiella . We concluded that SMF is a useful technique that, in combination with direct plating, including different culture media and incubation temperatures, improves the chance of recovering marine fungal communities in culture-dependent studies.
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معلومات مُعتمدة: PID2021-128068NB-100 Ministeri de Ciència Innovació i Universitats
فهرسة مساهمة: Keywords: Ascomycota; culture media; isolation; marine fungi; multi-locus phylogeny; taxonomy
تواريخ الأحداث: Date Created: 20240426 Latest Revision: 20240428
رمز التحديث: 20240428
مُعرف محوري في PubMed: PMC11051201
DOI: 10.3390/jof10040281
PMID: 38667952
قاعدة البيانات: MEDLINE
الوصف
تدمد:2309-608X
DOI:10.3390/jof10040281