دورية أكاديمية
NsdD, a GATA-type transcription factor is involved in regulation and biosynthesis of macromolecules melanin, pullulan, and polymalate in Aureobasidium melanogenum.
| العنوان: | NsdD, a GATA-type transcription factor is involved in regulation and biosynthesis of macromolecules melanin, pullulan, and polymalate in Aureobasidium melanogenum. |
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| المؤلفون: | Chi Z; College of Marine Life Sciences, Ocean University of China, Qingdao, China., Wei X; College of Marine Life Sciences, Ocean University of China, Qingdao, China., Ge N; College of Marine Life Sciences, Ocean University of China, Qingdao, China., Jiang H; College of Marine Life Sciences, Ocean University of China, Qingdao, China., Liu GL; College of Marine Life Sciences, Ocean University of China, Qingdao, China., Chi ZM; College of Marine Life Sciences, Ocean University of China, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address: chi@ouc.edu.cn. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2024 May; Vol. 268 (Pt 1), pp. 131820. Date of Electronic Publication: 2024 Apr 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Glucans*/biosynthesis , Glucans*/metabolism , Melanins*/biosynthesis , Aureobasidium*/metabolism , Aureobasidium*/genetics , Gene Expression Regulation, Fungal*, Fungal Proteins/genetics ; Fungal Proteins/metabolism ; GATA Transcription Factors/metabolism ; GATA Transcription Factors/genetics ; Mutation ; Transcription Factors/genetics ; Transcription Factors/metabolism |
| مستخلص: | In this study, an NSDD gene, which encoded a GATA-type transcription factor involved in the regulation and biosynthesis of melanin, pullulan, and polymalate (PMA) in Aureobasidium melanogenum, was characterized. After the NSDD gene was completely removed, melanin production by the Δnsd mutants was enhanced, while pullulan and polymalate production was significantly reduced. Transcription levels of the genes involved in melanin biosynthesis were up-regulated while expression levels of the genes responsible for pullulan and PMA biosynthesis were down-regulated in the Δnsdd mutants. In contrast, the complementation of the NSDD gene in the Δnsdd mutants made the overexpressing mutants restore melanin production and transcription levels of the genes responsible for melanin biosynthesis. Inversely, the complementation strains, compared to the wild type strains, showed enhanced pullulan and PMA yields. These results demonstrated that the NsdD was not only a negative regulator for melanin biosynthesis, but also a key positive regulator for pullulan and PMA biosynthesis in A. melanogenum. It was proposed how the same transcriptional factor could play a negative role in melanin biosynthesis and a positive role in pullulan and PMA biosynthesis. This study provided novel insights into the regulatory mechanisms of multiple A. melanogenum metabolites and the possibility for improving its yields of some industrial products through genetic approaches. Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aureobasidium melanogenum; GATA transcription factor; Melanin; NsdD; Polymalic acid; Pullulan |
| المشرفين على المادة: | 0 (pullulan) |
| SCR Organism: | Aureobasidium melanogenum |
| تواريخ الأحداث: | Date Created: 20240426 Date Completed: 20240521 Latest Revision: 20240528 |
| رمز التحديث: | 20240528 |
| DOI: | 10.1016/j.ijbiomac.2024.131820 |
| PMID: | 38670184 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2024.131820 |