دورية أكاديمية
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The endosymbiont of Epithemia clementina is specialized for nitrogen fixation within a photosynthetic eukaryote.

التفاصيل البيبلوغرافية
العنوان: The endosymbiont of Epithemia clementina is specialized for nitrogen fixation within a photosynthetic eukaryote.
المؤلفون: Moulin SLY; Department of Pathology, Stanford School of Medicine, Stanford, CA 94305, United States., Frail S; Department of Biochemistry, Stanford School of Medicine, Stanford, CA 94305, United States., Braukmann T; Department of Pathology, Stanford School of Medicine, Stanford, CA 94305, United States.; Department of Biochemistry, Stanford School of Medicine, Stanford, CA 94305, United States., Doenier J; Department of Biochemistry, Stanford School of Medicine, Stanford, CA 94305, United States., Steele-Ogus M; Department of Pathology, Stanford School of Medicine, Stanford, CA 94305, United States., Marks JC; Department of Biological Sciences, Northern Arizona University, Flagstaff, AR 86011, United States.; Center for Ecosystem Science and Society, Northern Arizona University, Flagstaff, AZ 86011, United States., Mills MM; Department of Earth System Science, Stanford Doerr School of Sustainability, Stanford, CA 94305, United States., Yeh E; Department of Pathology, Stanford School of Medicine, Stanford, CA 94305, United States.; Department of Microbiology & Immunology, Stanford School of Medicine, Stanford, CA 94305, United States.; Chan Zuckerberg Biohub, San Francisco, CA 94158, United States.
المصدر: ISME communications [ISME Commun] 2024 Apr 15; Vol. 4 (1), pp. ycae055. Date of Electronic Publication: 2024 Apr 15 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Nature on behalf of the International Society for Microbial Ecology Country of Publication: England NLM ID: 9918205372406676 Publication Model: eCollection Cited Medium: Internet ISSN: 2730-6151 (Electronic) Linking ISSN: 27306151 NLM ISO Abbreviation: ISME Commun Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [London] : Springer Nature on behalf of the International Society for Microbial Ecology, [2021]-
مستخلص: Epithemia spp. diatoms contain obligate, nitrogen-fixing endosymbionts, or diazoplasts, derived from cyanobacteria. These algae are a rare example of photosynthetic eukaryotes that have successfully coupled oxygenic photosynthesis with oxygen-sensitive nitrogenase activity. Here, we report a newly-isolated species, E. clementina , as a model to investigate endosymbiotic acquisition of nitrogen fixation. We demonstrate that the diazoplast, which has lost photosynthesis, provides fixed nitrogen to the diatom host in exchange for fixed carbon. To identify the metabolic changes associated with this endosymbiotic specialization, we compared the Epithemia diazoplast with its close, free-living cyanobacterial relative, Crocosphaera subtropica . Unlike C. subtropica , in which nitrogenase activity is temporally separated from photosynthesis, we show that nitrogenase activity in the diazoplast is continuous through the day (concurrent with host photosynthesis) and night. Host and diazoplast metabolism are tightly coupled to support nitrogenase activity: Inhibition of photosynthesis abolishes daytime nitrogenase activity, while nighttime nitrogenase activity no longer requires cyanobacterial glycogen storage pathways. Instead, import of host-derived carbohydrates supports nitrogenase activity throughout the day-night cycle. Carbohydrate metabolism is streamlined in the diazoplast compared to C. subtropica with retention of the oxidative pentose phosphate pathway and oxidative phosphorylation. Similar to heterocysts, these pathways may be optimized to support nitrogenase activity, providing reducing equivalents and ATP and consuming oxygen. Our results demonstrate that the diazoplast is specialized for endosymbiotic nitrogen fixation. Altogether, we establish a new model for studying endosymbiosis, perform a functional characterization of this diazotroph endosymbiosis, and identify metabolic adaptations for endosymbiotic acquisition of a critical biological function.
Competing Interests: The authors declare no competing interest.
(© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.)
التعليقات: Update of: bioRxiv. 2023 Apr 04;:. (PMID: 37066385)
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معلومات مُعتمدة: S10 OD028536 United States OD NIH HHS; T32 AI007328 United States AI NIAID NIH HHS; T32 GM007276 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: cyanobacteria; diatom; endosymbiosis; nitrogen fixation
تواريخ الأحداث: Date Created: 20240506 Latest Revision: 20240520
رمز التحديث: 20240520
مُعرف محوري في PubMed: PMC11070190
DOI: 10.1093/ismeco/ycae055
PMID: 38707843
قاعدة البيانات: MEDLINE
الوصف
تدمد:2730-6151
DOI:10.1093/ismeco/ycae055