دورية أكاديمية
A novel regulatory interplay between atypical B12 riboswitches and uORF translation in Mycobacterium tuberculosis.
العنوان: | A novel regulatory interplay between atypical B12 riboswitches and uORF translation in Mycobacterium tuberculosis. |
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المؤلفون: | Kipkorir T; Institute for Structural and Molecular Biology, University College London, Gower Street, WC1E 6BT London, UK., Polgar P; Institute for Structural and Molecular Biology, University College London, Gower Street, WC1E 6BT London, UK., Barker D; Institute for Structural and Molecular Biology, University College London, Gower Street, WC1E 6BT London, UK., D'Halluin A; Institute for Structural and Molecular Biology, University College London, Gower Street, WC1E 6BT London, UK., Patel Z; Institute for Structural and Molecular Biology, University College London, Gower Street, WC1E 6BT London, UK., Arnvig KB; Institute for Structural and Molecular Biology, University College London, Gower Street, WC1E 6BT London, UK. |
المصدر: | Nucleic acids research [Nucleic Acids Res] 2024 Jul 22; Vol. 52 (13), pp. 7876-7892. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 1992- : Oxford : Oxford University Press Original Publication: London, Information Retrieval ltd. |
مواضيع طبية MeSH: | Riboswitch*/genetics , Mycobacterium tuberculosis*/genetics , Mycobacterium tuberculosis*/metabolism , Gene Expression Regulation, Bacterial* , Protein Biosynthesis*/genetics , Open Reading Frames*/genetics , Vitamin B 12*/metabolism, Nucleic Acid Conformation ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Ligands ; Base Sequence ; RNA, Bacterial/metabolism ; RNA, Bacterial/genetics |
مستخلص: | Vitamin B12 is an essential cofactor in all domains of life and B12-sensing riboswitches are some of the most widely distributed riboswitches. Mycobacterium tuberculosis, the causative agent of tuberculosis, harbours two B12-sensing riboswitches. One controls expression of metE, encoding a B12-independent methionine synthase, the other controls expression of ppe2 of uncertain function. Here, we analysed ligand sensing, secondary structure and gene expression control of the metE and ppe2 riboswitches. Our results provide the first evidence of B12 binding by these riboswitches and show that they exhibit different preferences for individual isoforms of B12, use distinct regulatory and structural elements and act as translational OFF switches. Based on our results, we propose that the ppe2 switch represents a new variant of Class IIb B12-sensing riboswitches. Moreover, we have identified short translated open reading frames (uORFs) upstream of metE and ppe2, which modulate the expression of their downstream genes. Translation of the metE uORF suppresses MetE expression, while translation of the ppe2 uORF is essential for PPE2 expression. Our findings reveal an unexpected regulatory interplay between B12-sensing riboswitches and the translational machinery, highlighting a new level of cis-regulatory complexity in M. tuberculosis. Attention to such mechanisms will be critical in designing next-level intervention strategies. (© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.) |
References: | Annu Rev Microbiol. 2017 Sep 8;71:263-280. (PMID: 28886688) Curr Biol. 2003 Apr 1;13(7):R285-91. (PMID: 12676109) Nucleic Acids Res. 2004 Jan 02;32(1):143-50. (PMID: 14704351) RNA Biol. 2019 Dec;16(12):1667-1671. (PMID: 31441369) Nucleic Acids Res. 2020 Jul 27;48(13):7569-7583. (PMID: 32544228) Tuberculosis (Edinb). 2013 Jan;93(1):18-25. (PMID: 23273318) J Bacteriol. 1997 Jun;179(12):4039-42. (PMID: 9190822) Open Biol. 2013 Feb 13;3(2):120175. (PMID: 23407640) Mol Microbiol. 2015 Jun;96(5):901-16. (PMID: 25727695) Mol Microbiol. 1992 Mar;6(6):743-9. (PMID: 1374146) PLoS Med. 2016 Oct 25;13(10):e1002152. (PMID: 27780211) Microbiol Spectr. 2016 Oct;4(5):. (PMID: 27787194) Infect Genet Evol. 2014 Mar;22:244-9. (PMID: 23827809) J Biol Chem. 2020 Jul 24;295(30):10434-10445. (PMID: 32518156) BMC Bioinformatics. 2010 Mar 15;11:129. (PMID: 20230624) Elife. 2022 Mar 28;11:. (PMID: 35343439) J Bacteriol. 2007 May;189(9):3655-9. (PMID: 17307844) Biochim Biophys Acta. 1963 Sep 17;76:162-4. (PMID: 14068553) Nat Commun. 2019 Sep 5;10(1):4006. (PMID: 31488843) Annu Rev Genet. 2020 Nov 23;54:237-264. (PMID: 32870728) Nucleic Acids Res. 2015 Jan;43(Database issue):D130-7. (PMID: 25392425) RNA Biol. 2018;15(6):679-682. (PMID: 29537923) Front Mol Biosci. 2015 Mar 04;2:6. (PMID: 25988174) PLoS One. 2017 Mar 21;12(3):e0174079. (PMID: 28323872) Cold Spring Harb Perspect Biol. 2018 Nov 1;10(11):. (PMID: 29844057) Sci Rep. 2021 Jun 10;11(1):12267. (PMID: 34112827) Nat Commun. 2020 Jun 9;11(1):2917. (PMID: 32518235) Front Cell Infect Microbiol. 2020 Nov 26;10:594288. (PMID: 33324577) J Mol Biol. 2000 Sep 8;302(1):205-17. (PMID: 10964570) Front Cell Infect Microbiol. 2019 Oct 09;9:337. (PMID: 31649894) BMC Genomics. 2009 Feb 10;10:78. (PMID: 19208259) Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9736-41. (PMID: 11470904) Chem Biol. 2004 Dec;11(12):1729-41. (PMID: 15610857) Microbiol Spectr. 2018 Jul;6(4):. (PMID: 30003867) EMBO J. 2011 Apr 20;30(8):1485-96. (PMID: 21399613) Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5376-81. (PMID: 22431636) Immunobiology. 2023 Mar;228(2):152321. (PMID: 36805109) RNA. 2017 Jul;23(7):995-1011. (PMID: 28396576) Cell. 2007 Sep 7;130(5):878-92. (PMID: 17803910) Gene. 1992 Dec 1;122(1):231-2. (PMID: 1339377) Future Microbiol. 2013 Nov;8(11):1405-18. (PMID: 24199800) Proc Natl Acad Sci U S A. 2018 Jun 5;115(23):6052-6057. (PMID: 29784782) Nat Genet. 2013 Feb;45(2):172-9. (PMID: 23291586) Wiley Interdiscip Rev RNA. 2017 Nov;8(6):. (PMID: 28792118) PLoS Genet. 2015 Nov 04;11(11):e1005641. (PMID: 26536359) Bioinformatics. 2009 May 1;25(9):1189-91. (PMID: 19151095) Nat Prod Rep. 2002 Aug;19(4):390-412. (PMID: 12195810) Mol Cell. 2013 Dec 12;52(5):629-42. (PMID: 24239289) Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):15908-13. (PMID: 12456892) Biochim Biophys Acta. 2009 Sep-Oct;1789(9-10):584-91. (PMID: 19595806) iScience. 2023 Mar 22;26(4):106465. (PMID: 37096044) Proc Natl Acad Sci U S A. 2018 Apr 10;115(15):3894-3899. (PMID: 29581302) Methods Mol Biol. 2008;419:53-67. (PMID: 18369975) Pathog Dis. 2018 Jun 1;76(4):. (PMID: 29796669) Cold Spring Harb Perspect Biol. 2012 Feb 01;4(2):. (PMID: 21106649) Biochem Soc Trans. 2019 Aug 30;47(4):1091-1099. (PMID: 31249101) Annu Rev Biochem. 2003;72:209-47. (PMID: 14527323) J Bacteriol. 2008 Feb;190(4):1160-71. (PMID: 17981976) Proc Natl Acad Sci U S A. 2013 Apr 30;110(18):7240-5. (PMID: 23589841) RNA. 2003 Sep;9(9):1084-97. (PMID: 12923257) J Biol Chem. 2003 Oct 17;278(42):41148-59. (PMID: 12869542) J Biol Chem. 2017 Jul 14;292(28):11650-11658. (PMID: 28483920) Sci Rep. 2017 Jan 10;7:39706. (PMID: 28071726) Biochemistry (Mosc). 2021 Aug;86(8):962-975. (PMID: 34488573) Nucleic Acids Res. 2020 Feb 20;48(3):1029-1042. (PMID: 31504789) Nature. 2012 Dec 6;492(7427):133-7. (PMID: 23064232) Nat Commun. 2024 Mar 9;15(1):2161. (PMID: 38461302) J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712) Nat Struct Mol Biol. 2012 Nov;19(11):1182-4. (PMID: 23064646) Nature. 2023 Feb;614(7947):367-374. (PMID: 36697824) Science. 2004 Oct 8;306(5694):275-9. (PMID: 15472076) mBio. 2017 Aug 29;8(4):. (PMID: 28851853) J Bacteriol. 2021 Mar 8;203(7):. (PMID: 33468593) Immunobiology. 2021 Jan;226(1):152051. (PMID: 33352401) Biochim Biophys Acta. 2014 Oct;1839(10):974-977. (PMID: 24731855) Annu Rev Genet. 2022 Nov 30;56:187-205. (PMID: 36055649) |
معلومات مُعتمدة: | MR/S009647/1 UK Medical Research Council; NIF\R1\180833 Newton International Fellowship; 204841/Z/16/Z Wellcome Institutional Strategic Support Fund; 225605/Z/22/Z Wellcome Early Career Award; United Kingdom WT_ Wellcome Trust |
المشرفين على المادة: | 0 (Riboswitch) P6YC3EG204 (Vitamin B 12) 0 (Bacterial Proteins) 0 (Ligands) 0 (RNA, Bacterial) |
تواريخ الأحداث: | Date Created: 20240506 Date Completed: 20240721 Latest Revision: 20240723 |
رمز التحديث: | 20240723 |
مُعرف محوري في PubMed: | PMC11260477 |
DOI: | 10.1093/nar/gkae338 |
PMID: | 38709884 |
قاعدة البيانات: | MEDLINE |
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