دورية أكاديمية
أعرض في EDS

A non-canonical role of the inner kinetochore in regulating sister-chromatid cohesion at centromeres.

التفاصيل البيبلوغرافية
العنوان: A non-canonical role of the inner kinetochore in regulating sister-chromatid cohesion at centromeres.
المؤلفون: Yan L; Life Sciences Institute, State Key Laboratory of Transvascular Implantation Devices of the Second Affiliated Hospital of Zhejiang University School of Medicine, MOE Laboratory of Biosystems Homeostasis and Protection, Zhejiang University, Hangzhou, 310058, China., Yuan X; Life Sciences Institute, State Key Laboratory of Transvascular Implantation Devices of the Second Affiliated Hospital of Zhejiang University School of Medicine, MOE Laboratory of Biosystems Homeostasis and Protection, Zhejiang University, Hangzhou, 310058, China., Liu M; Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China., Chen Q; Department of Gynecological Oncology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China., Zhang M; Life Sciences Institute, State Key Laboratory of Transvascular Implantation Devices of the Second Affiliated Hospital of Zhejiang University School of Medicine, MOE Laboratory of Biosystems Homeostasis and Protection, Zhejiang University, Hangzhou, 310058, China., Xu J; Department of Gynecological Oncology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China., Zeng LH; Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province, School of Medicine, Hangzhou City University, Hangzhou, 310015, China., Zhang L; Life Sciences Institute, State Key Laboratory of Transvascular Implantation Devices of the Second Affiliated Hospital of Zhejiang University School of Medicine, MOE Laboratory of Biosystems Homeostasis and Protection, Zhejiang University, Hangzhou, 310058, China.; Cancer Center, Zhejiang University, Hangzhou, 310058, China., Huang J; Life Sciences Institute, State Key Laboratory of Transvascular Implantation Devices of the Second Affiliated Hospital of Zhejiang University School of Medicine, MOE Laboratory of Biosystems Homeostasis and Protection, Zhejiang University, Hangzhou, 310058, China.; Cancer Center, Zhejiang University, Hangzhou, 310058, China., Lu W; Department of Gynecological Oncology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China.; Cancer Center, Zhejiang University, Hangzhou, 310058, China., He X; Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China. hexj@hust.edu.cn., Yan H; Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province, School of Medicine, Hangzhou City University, Hangzhou, 310015, China. yanhy@hzcu.edu.cn., Wang F; Life Sciences Institute, State Key Laboratory of Transvascular Implantation Devices of the Second Affiliated Hospital of Zhejiang University School of Medicine, MOE Laboratory of Biosystems Homeostasis and Protection, Zhejiang University, Hangzhou, 310058, China. fwwang@zju.edu.cn.; Department of Gynecological Oncology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China. fwwang@zju.edu.cn.; Cancer Center, Zhejiang University, Hangzhou, 310058, China. fwwang@zju.edu.cn.; Zhejiang Provincial Key Laboratory of Geriatrics and Geriatrics Institute of Zhejiang Province, Affiliated Zhejiang Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China. fwwang@zju.edu.cn.
المصدر: The EMBO journal [EMBO J] 2024 Jun; Vol. 43 (12), pp. 2424-2452. Date of Electronic Publication: 2024 May 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley Blackwell Country of Publication: England NLM ID: 8208664 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2075 (Electronic) Linking ISSN: 02614189 NLM ISO Abbreviation: EMBO J Subsets: MEDLINE
أسماء مطبوعة: Publication: 2014- : London : Wiley Blackwell
Original Publication: Eynsham, Oxford, England : Published for the European Molecular Biology Organization by IRL Press, [c1982-
مواضيع طبية MeSH: Kinetochores*/metabolism , Chromosomal Proteins, Non-Histone*/metabolism , Chromosomal Proteins, Non-Histone*/genetics , Cell Cycle Proteins*/metabolism , Cell Cycle Proteins*/genetics , Chromatids*/metabolism , Chromatids*/genetics , Centromere*/metabolism, Humans ; Cohesins ; HeLa Cells ; Protein Binding ; Crystallography, X-Ray
مستخلص: The 16-subunit Constitutive Centromere-associated Network (CCAN)-based inner kinetochore is well-known for connecting centromeric chromatin to the spindle-binding outer kinetochore. Here, we report a non-canonical role for the inner kinetochore in directly regulating sister-chromatid cohesion at centromeres. We provide biochemical, X-ray crystal structure, and intracellular ectopic localization evidence that the inner kinetochore directly binds cohesin, a ring-shaped multi-subunit complex that holds sister chromatids together from S-phase until anaphase onset. This interaction is mediated by binding of the 5-subunit CENP-OPQUR sub-complex of CCAN to the Scc1-SA2 sub-complex of cohesin. Mutation in the CENP-U subunit of the CENP-OPQUR complex that abolishes its binding to the composite interface between Scc1 and SA2 weakens centromeric cohesion, leading to premature separation of sister chromatids during delayed metaphase. We further show that CENP-U competes with the cohesin release factor Wapl for binding the interface of Scc1-SA2, and that the cohesion-protecting role for CENP-U can be bypassed by depleting Wapl. Taken together, this study reveals an inner kinetochore-bound pool of cohesin, which strengthens centromeric sister-chromatid cohesion to resist metaphase spindle pulling forces.
(© 2024. The Author(s).)
References: J Cell Biol. 2008 Dec 15;183(6):1019-31. (PMID: 19075111)
J Cell Biol. 2020 Mar 2;219(3):. (PMID: 32028528)
Trends Cell Biol. 2016 Sep;26(9):680-693. (PMID: 27134029)
J Cell Sci. 2011 Sep 1;124(Pt 17):2976-87. (PMID: 21878504)
Cell. 2004 Mar 5;116(5):683-98. (PMID: 15006351)
Cell. 2004 Sep 3;118(5):567-78. (PMID: 15339662)
Curr Biol. 2006 May 9;16(9):863-74. (PMID: 16682347)
Genes Dev. 2002 Dec 1;16(23):3004-16. (PMID: 12464631)
Genes Dev. 2009 Sep 15;23(18):2224-36. (PMID: 19696148)
Curr Biol. 2013 Oct 7;23(19):1927-33. (PMID: 24055156)
Nature. 2014 Oct 30;514(7524):646-9. (PMID: 25156254)
Cell. 2018 Jan 25;172(3):465-477.e15. (PMID: 29358048)
Elife. 2021 Feb 17;10:. (PMID: 33594972)
Curr Biol. 2004 Jul 13;14(13):1187-93. (PMID: 15242616)
Mol Cell. 2018 Sep 20;71(6):923-939.e10. (PMID: 30174292)
Nat Genet. 2004 Jun;36(6):636-41. (PMID: 15146185)
Science. 2010 Oct 8;330(6001):235-9. (PMID: 20705815)
Mol Cell. 2020 Sep 17;79(6):902-916.e6. (PMID: 32768407)
Nat Struct Mol Biol. 2023 Jun;30(6):853-859. (PMID: 37081319)
Proc Natl Acad Sci U S A. 2013 Aug 13;110(33):13404-9. (PMID: 23901111)
Nat Cell Biol. 2011 Oct 03;13(10):1170-7. (PMID: 21968990)
Nat Rev Mol Cell Biol. 2023 Sep;24(9):633-650. (PMID: 37231112)
Mol Cell. 2002 Mar;9(3):515-25. (PMID: 11931760)
Nat Rev Mol Cell Biol. 2021 Jul;22(7):445-464. (PMID: 33767413)
Dev Cell. 2014 Oct 13;31(1):7-18. (PMID: 25313959)
J Biol Chem. 2019 Feb 1;294(5):1437-1450. (PMID: 30498087)
Nat Cell Biol. 2010 Apr;12(4):319-29. (PMID: 20228811)
Cell. 1997 Oct 3;91(1):47-57. (PMID: 9335334)
Front Mol Biosci. 2022 Oct 18;9:1010894. (PMID: 36330215)
Prog Mol Subcell Biol. 2017;56:485-513. (PMID: 28840250)
EMBO J. 2017 Dec 15;36(24):3573-3599. (PMID: 29217591)
Cell. 2017 May 4;169(4):693-707.e14. (PMID: 28475897)
Mol Cell. 2015 Aug 6;59(3):426-36. (PMID: 26190260)
Nat Rev Mol Cell Biol. 2016 Jan;17(1):16-29. (PMID: 26601620)
Cell. 1997 Oct 3;91(1):35-45. (PMID: 9335333)
Curr Opin Cell Biol. 2018 Jun;52:73-81. (PMID: 29477052)
Curr Biol. 2006 Dec 19;16(24):2406-17. (PMID: 17112726)
Curr Biol. 2013 Oct 21;23(20):2071-7. (PMID: 24055153)
PLoS One. 2013 Jul 12;8(7):e69458. (PMID: 23874961)
Nature. 2008 Jul 17;454(7202):297-301. (PMID: 18596691)
Science. 2010 Oct 8;330(6001):239-43. (PMID: 20929775)
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11355-60. (PMID: 23776203)
Cell. 2006 Dec 1;127(5):955-67. (PMID: 17113138)
Philos Trans R Soc Lond B Biol Sci. 2005 Mar 29;360(1455):537-42. (PMID: 15897179)
Mol Cell. 2021 Jan 7;81(1):67-87.e9. (PMID: 33248027)
Nature. 2020 Feb;578(7795):472-476. (PMID: 31905366)
Science. 2010 Oct 8;330(6001):231-5. (PMID: 20705812)
Cell. 2017 Sep 21;171(1):72-84.e13. (PMID: 28938124)
PLoS Biol. 2005 Mar;3(3):e86. (PMID: 15737064)
Genes Dev. 1998 Jul 1;12(13):1986-97. (PMID: 9649503)
Cold Spring Harb Perspect Biol. 2015 Feb 26;7(6):. (PMID: 25722466)
Curr Biol. 2017 Apr 3;27(7):1005-1012. (PMID: 28343969)
J Cell Biol. 2020 Feb 3;219(2):. (PMID: 31868888)
Curr Biol. 2013 Nov 18;23(22):R986-R988. (PMID: 24262830)
Mol Biol Cell. 2021 Apr 15;32(8):712-721. (PMID: 33596090)
Cell Discov. 2022 Sep 9;8(1):90. (PMID: 36085283)
Mol Cell. 2006 Nov 3;24(3):409-22. (PMID: 17081991)
PLoS Biol. 2005 Mar;3(3):e69. (PMID: 15737063)
Trends Genet. 2020 Apr;36(4):232-242. (PMID: 32155445)
Nat Genet. 2004 Jun;36(6):631-5. (PMID: 15146186)
Science. 2010 Jan 8;327(5962):172-7. (PMID: 19965387)
J Biol Chem. 2011 Jan 14;286(2):1627-38. (PMID: 21056971)
Nat Struct Mol Biol. 2014 Oct;21(10):864-70. (PMID: 25173175)
J Mol Cell Biol. 2022 Sep 27;14(7):. (PMID: 36069839)
Mol Cell. 2000 Feb;5(2):243-54. (PMID: 10882066)
Curr Biol. 2005 Feb 22;15(4):353-9. (PMID: 15723797)
Curr Biol. 2017 Apr 3;27(7):992-1004. (PMID: 28343965)
Mol Biol Cell. 2008 Mar;19(3):843-54. (PMID: 18094054)
Mol Cell. 2022 Jun 2;82(11):2113-2131.e8. (PMID: 35525244)
Science. 2022 May 20;376(6595):844-852. (PMID: 35420891)
Nature. 2004 Feb 5;427(6974):510-7. (PMID: 14730319)
Mol Biol Cell. 2018 Aug 1;29(15):1811-1824. (PMID: 29846129)
Nat Rev Mol Cell Biol. 2023 Aug;24(8):543-559. (PMID: 36964313)
J Cell Biol. 2020 Mar 2;219(3):. (PMID: 32027339)
Cell. 2000 Oct 27;103(3):375-86. (PMID: 11081625)
J Cell Sci. 2007 Dec 1;120(Pt 23):4188-96. (PMID: 18003702)
Nature. 2013 Sep 26;501(7468):564-8. (PMID: 23975099)
J Cell Sci. 2015 Jan 1;128(1):171-84. (PMID: 25395579)
J Cell Sci. 1999 Aug;112 ( Pt 16):2607-13. (PMID: 10413669)
Cell Rep. 2021 Sep 21;36(12):109740. (PMID: 34551298)
Nat Rev Cancer. 2021 Feb;21(2):89-103. (PMID: 33432169)
Nat Cell Biol. 2013 Jan;15(1):40-9. (PMID: 23242214)
Curr Biol. 2011 Jun 21;21(12):1018-24. (PMID: 21658943)
Proc Natl Acad Sci U S A. 2004 Dec 28;101(52):18012-7. (PMID: 15604152)
معلومات مُعتمدة: 32025011,32061160470 MOST | National Natural Science Foundation of China (NSFC); 32270772 MOST | National Natural Science Foundation of China (NSFC); 31970672,32270877 MOST | National Natural Science Foundation of China (NSFC); 32100583 MOST | National Natural Science Foundation of China (NSFC); 32200580 MOST | National Natural Science Foundation of China (NSFC); 31925013 MOST | National Natural Science Foundation of China (NSFC); 2022YFA1303102,2022YFA1105203,2017YFA0503602 MOST | National Key Research and Development Program of China (NKPs); LZ24C070001,LZ19C070001 MOST | NSFC | NSFC-Zhejiang Joint Fund | | Natural Science Foundation of Zhejiang Province (ZJNSF); LY17C070003 MOST | NSFC | NSFC-Zhejiang Joint Fund | | Natural Science Foundation of Zhejiang Province (ZJNSF); NA140075 Newton Fund (NF); 2020M681838 China Postdoctoral Foundation Project | Postdoctoral Research Foundation of China (China Postdoctoral Research Foundation); 2022M720126,2021M692829 China Postdoctoral Foundation Project | Postdoctoral Research Foundation of China (China Postdoctoral Research Foundation)
فهرسة مساهمة: Keywords: Centromere; Cohesin; Kinetochore; Mitosis; Sister-Chromatid Cohesion
المشرفين على المادة: 0 (Chromosomal Proteins, Non-Histone)
0 (Cell Cycle Proteins)
0 (Cohesins)
تواريخ الأحداث: Date Created: 20240507 Date Completed: 20240617 Latest Revision: 20240620
رمز التحديث: 20240620
مُعرف محوري في PubMed: PMC11182772
DOI: 10.1038/s44318-024-00104-6
PMID: 38714893
قاعدة البيانات: MEDLINE
الوصف
تدمد:1460-2075
DOI:10.1038/s44318-024-00104-6