دورية أكاديمية
Salidroside ameliorates acute liver transplantation rejection in rats by inhibiting neutrophil extracellular trap formation.
| العنوان: | Salidroside ameliorates acute liver transplantation rejection in rats by inhibiting neutrophil extracellular trap formation. |
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| المؤلفون: | Qin X; Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, China.; Department of General Surgery and Trauma Surgery, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Structural Birth Defect and Reconstruction, Chongqing 400014, China., Wang H; Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, China., Li Q; Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, China., Hu D; Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, China., Wang L; Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, China., Zhou B; Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, China., Liao R; Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, China., Liu Y; Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, China. |
| المصدر: | Acta biochimica et biophysica Sinica [Acta Biochim Biophys Sin (Shanghai)] 2024 Jun 25; Vol. 56 (6), pp. 833-843. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: China Science Publishing & Media Ltd Country of Publication: China NLM ID: 101206716 Publication Model: Print Cited Medium: Internet ISSN: 1745-7270 (Electronic) Linking ISSN: 16729145 NLM ISO Abbreviation: Acta Biochim Biophys Sin (Shanghai) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2022- : Shanghai : China Science Publishing & Media Ltd. Original Publication: Shanghai : Shanghai Scientific and Technical Publishers, 2004- |
| مواضيع طبية MeSH: | Phenols*/pharmacology , Glucosides*/pharmacology , Liver Transplantation* , Extracellular Traps*/drug effects , Extracellular Traps*/metabolism , Graft Rejection*/prevention & control , Graft Rejection*/pathology , Graft Rejection*/drug therapy , Graft Rejection*/metabolism , HMGB1 Protein*/metabolism , Toll-Like Receptor 4*/metabolism , Neutrophils*/drug effects , Neutrophils*/metabolism, Animals ; Male ; Rats ; Rats, Sprague-Dawley ; Liver/drug effects ; Liver/pathology ; Liver/metabolism ; MAP Kinase Signaling System/drug effects ; Apoptosis/drug effects |
| مستخلص: | Acute rejection is an important factor affecting the survival of recipients after liver transplantation. Salidroside has various properties, including anti-inflammatory, antioxidant, and hepatoprotective properties. This study aims to investigate whether salidroside can prevent acute rejection after liver transplantation and to examine the underlying mechanisms involved. An in vivo acute rejection model is established in rats that are pretreated with tacrolimus (1 mg/kg/d) or salidroside (10 or 20 mg/kg/d) for seven days after liver transplantation. In addition, an in vitro experiment is performed using neutrophils incubated with salidroside (1, 10, 50 or 100 μM). Hematoxylin-eosin staining, terminal deoxynucleotidyl transferase dUTP nick-end labeling staining, immunosorbent assays, immunofluorescence analysis, Evans blue staining, and western blot analysis are performed to examine the impact of salidroside on NET formation and acute rejection in vitro and in vivo . We find that Salidroside treatment reduces pathological liver damage, serum aminotransferase level, and serum levels of IL-1β, IL-6, and TNF-α in vivo . The expressions of proteins associated with the HMGB1/TLR-4/MAPK signaling pathway (HMGB1, TLR-4, p-ERK1/2, p-JNK, p-P38, cleaved caspase-3, cleaved caspase-9, Bcl-2, Bax, IL-1β, TNF-α, and IL-6) are also decreased after salidroside treatment. In vitro experiments show that the release of HMGB1/TLR-4/MAPK signaling pathway-associated proteins from neutrophils treated with lipopolysaccharide is decreased by salidroside. Moreover, salidroside inhibits NETosis and protects against acute rejection by regulating the HMGB1/TLR-4/MAPK signaling pathway. Furthermore, salidroside combined with tacrolimus has a better effect than either of the other treatments alone. In summary, salidroside can prevent acute liver rejection after liver transplantation by reducing neutrophil extracellular trap development through the HMGB1/TLR-4/MAPK signaling pathway. |
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| فهرسة مساهمة: | Keywords: HMGB1; acute rejection; liver transplantation; neutrophil extracellular traps; salidroside |
| المشرفين على المادة: | M983H6N1S9 (rhodioloside) 0 (Phenols) 0 (Glucosides) 0 (HMGB1 Protein) 0 (Toll-Like Receptor 4) 0 (Tlr4 protein, rat) 0 (Hbp1 protein, rat) |
| تواريخ الأحداث: | Date Created: 20240508 Date Completed: 20240613 Latest Revision: 20240703 |
| رمز التحديث: | 20240703 |
| مُعرف محوري في PubMed: | PMC11214976 |
| DOI: | 10.3724/abbs.2024055 |
| PMID: | 38716542 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1745-7270 |
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| DOI: | 10.3724/abbs.2024055 |