دورية أكاديمية
أعرض في EDS

Designing and synthesis of injectable hydrogel based on carboxymethyl cellulose/carboxymethyl chitosan containing QK peptide for femoral head osteonecrosis healing.

التفاصيل البيبلوغرافية
العنوان: Designing and synthesis of injectable hydrogel based on carboxymethyl cellulose/carboxymethyl chitosan containing QK peptide for femoral head osteonecrosis healing.
المؤلفون: Peyravian N; Cellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran., Milan PB; Cellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address: boroukimilan.t@iums.ac.ir., Kebria MM; Cellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran., Mashayekhan S; Department of Chemical and Petroleum Engineering, Sharif University of Technology, Azadi Ave., Tehran, Iran., Ghasemian M; Cellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran., Amiri S; Shohadaye Haftom-e-tir Hospital, Department of Orthopedics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran., Hamidi M; Université Libre de Bruxelles (ULB), École polytechnique de Bruxelles - 3BIO-BioMatter unit, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050 Brussels, Belgium., Shavandi A; Université Libre de Bruxelles (ULB), École polytechnique de Bruxelles - 3BIO-BioMatter unit, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050 Brussels, Belgium., Moghtadaei M; Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran; Orthopaedic Department, Hazrat-Rasul Hospital, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address: moghtadaei.m@iums.ac.ir.
المصدر: International journal of biological macromolecules [Int J Biol Macromol] 2024 Jun; Vol. 270 (Pt 1), pp. 132127. Date of Electronic Publication: 2024 May 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
مواضيع طبية MeSH: Chitosan*/chemistry , Chitosan*/analogs & derivatives , Chitosan*/pharmacology , Hydrogels*/chemistry , Hydrogels*/pharmacology , Hydrogels*/chemical synthesis , Carboxymethylcellulose Sodium*/chemistry , Carboxymethylcellulose Sodium*/pharmacology , Femur Head Necrosis*/drug therapy , Femur Head Necrosis*/pathology , Human Umbilical Vein Endothelial Cells*/drug effects, Animals ; Humans ; Peptides/chemistry ; Peptides/pharmacology ; Peptides/chemical synthesis ; Osteogenesis/drug effects ; Mesenchymal Stem Cells/drug effects ; Mesenchymal Stem Cells/metabolism ; Cell Proliferation/drug effects ; Wound Healing/drug effects ; Injections ; Neovascularization, Physiologic/drug effects ; Cell Differentiation/drug effects ; Male ; Rabbits ; Disease Models, Animal
مستخلص: Femoral head necrosis is a debilitating disorder that typically caused by impaired blood supply to the hip joint. In this study, a novel injectable hydrogel based on Oxidized Carboxymethyl Cellulose (OCMC)-Carboxymethyl Chitosan (CMCS) polymers containing an angiogenesis stimulator peptide (QK) with a non-toxic crosslinking interaction (Schiff based reaction) was synthesized to enhance angiogenesis following femoral head necrosis in an animal model. The physicochemical features of fabricated injectable hydrogel were analyzed by FTIR, swelling and degradation rate, rheometry, and peptide release. Also, the safety and efficacy were evaluated following an in vitro hydrogel injection study and an avascular necrosis (AVN) animal model. According to the results, the hydrogel exhibited an appropriate swelling ratio and water uptake (>90 %, 24 h) as well as a suitable degradation rate over 21 days accompanied by a continuous peptide release. Also, data showed that hydrogels containing QK peptide boosted the proliferation, differentiation, angiogenesis, and osteogenic potential of both Bone Marrow mesenchymal Stem Cells (BM-MSCs) and human umbilical vein endothelial cells (HUVECs) (****p < 0.0001 and ***p < 0.001, respectively). Furthermore, molecular and histological evaluations significantly demonstrated the overexpression of Runx2, Osteocalcin, Collagen I, VEGF and CD34 genes (**p < 0.01 and ***p < 0.001, respectively), and also femoral head necrosis was effectively prohibited, and more blood vessels were detected in defect area by OCMC-CMCS hydrogel containing QK peptide (bone trabeculae >9000, ***p < 0.001). In conclusion, the findings demonstrate that OCMC-CMCS-QK injectable hydrogel could be considered as an impressive therapeutic construct for femoral head AVN healing.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Carboxymethyl chitosan; Femoral head necrosis; Injectable hydrogels; Oxidized carboxymethyl cellulose; QK peptide
المشرفين على المادة: 9012-76-4 (Chitosan)
0 (Hydrogels)
K679OBS311 (Carboxymethylcellulose Sodium)
0 (carboxymethyl-chitosan)
0 (Peptides)
تواريخ الأحداث: Date Created: 20240508 Date Completed: 20240604 Latest Revision: 20240604
رمز التحديث: 20240604
DOI: 10.1016/j.ijbiomac.2024.132127
PMID: 38718991
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0003
DOI:10.1016/j.ijbiomac.2024.132127