دورية أكاديمية
أعرض في EDS

Application of environmental DNA metabarcoding to identify fish community characteristics in subtropical river systems.

التفاصيل البيبلوغرافية
العنوان: Application of environmental DNA metabarcoding to identify fish community characteristics in subtropical river systems.
المؤلفون: Wang S; State Key Laboratory of Marine Resource Utilization in South China Sea Hainan University Haikou China., Wu DH; State Key Laboratory of Marine Resource Utilization in South China Sea Hainan University Haikou China.; College of Ecology and Environment Hainan University Haikou China., Song YD; State Key Laboratory of Marine Resource Utilization in South China Sea Hainan University Haikou China., Wang TT; College of Ecology and Environment Hainan University Haikou China.; Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province Hainan University Haikou China., Fan SD; State Key Laboratory of Marine Resource Utilization in South China Sea Hainan University Haikou China., Wu EN; State Key Laboratory of Marine Resource Utilization in South China Sea Hainan University Haikou China., Chen NL; State Key Laboratory of Marine Resource Utilization in South China Sea Hainan University Haikou China., Xia WT; State Key Laboratory of Marine Resource Utilization in South China Sea Hainan University Haikou China., Xu MN; State Key Laboratory of Marine Resource Utilization in South China Sea Hainan University Haikou China., Chen ZB; Department of Applied·Ecology, Faculty of Environmental Sciences Czech University of Life Sciences Prague Praha-Suchdol Czech Republic., Wen J; State Key Laboratory of Marine Resource Utilization in South China Sea Hainan University Haikou China.; Hainan Qingxiao Environmental Testing Co., Ltd. Sanya China.; Hainan Qianchao Ecological Technology Co., Ltd. Sanya China., Zhang Y; Shenzhen Guanghuiyuan Environment Water Co., Ltd. Shenzhen China., Mo L; Hainan Research Academy of Environmental Sciences Haikou China., Xiang L; Department of Ecology Jinan University Guangzhou China.
المصدر: Ecology and evolution [Ecol Evol] 2024 May 09; Vol. 14 (5), pp. e11214. Date of Electronic Publication: 2024 May 09 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Blackwell Pub. Ltd Country of Publication: England NLM ID: 101566408 Publication Model: eCollection Cited Medium: Print ISSN: 2045-7758 (Print) Linking ISSN: 20457758 NLM ISO Abbreviation: Ecol Evol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Oxford] : Blackwell Pub. Ltd.
مستخلص: Fish are vital in river ecosystems; however, traditional investigations of fish usually cause ecological damage. Extracting DNA from aquatic environments and identifying DNA sequences offer an alternative, noninvasive approach for detecting fish species. In this study, the effects of environmental DNA (eDNA), coupled with PCR and next-generation sequencing, and electrofishing for identifying fish community composition and diversity were compared. In three subtropical rivers of southern China, fish specimens and eDNA in water were collected along the longitudinal (upstream-downstream) gradient of the rivers. Both fish population parameters, including species abundance and biomass, and eDNA OTU richness grouped 38 sampling sites into eight spatial zones with significant differences in local fish community composition. Compared with order-/family-level grouping, genus-/species-level grouping could more accurately reveal the differences between upstream zones I-III, midstream zones IV-V, and downstream zones VI-VIII. From the headwaters to the estuary, two environmental gradients significantly influenced the longitudinal distribution of the fish species, including the first gradient composed of habitat and physical water parameters and the second gradient composed of chemical water parameters. The high regression coefficient of alpha diversity between eDNA and electrofishing methods as well as the accurate identification of dominant, alien, and biomarker species in each spatial zone indicated that eDNA could characterize fish community attributes at a level similar to that of traditional approaches. Overall, our results demonstrated that eDNA metabarcoding can be used as an effective tool for revealing fish composition and diversity, which is important for using the eDNA technique in aquatic field monitoring.
Competing Interests: The authors declare that they have no competing interests.
(© 2024 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.)
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فهرسة مساهمة: Keywords: biomarker; fish community; high‐throughput sequencing; metabarcoding; monitoring tool; river food web
تواريخ الأحداث: Date Created: 20240510 Latest Revision: 20240511
رمز التحديث: 20240511
مُعرف محوري في PubMed: PMC11079634
DOI: 10.1002/ece3.11214
PMID: 38725828
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-7758
DOI:10.1002/ece3.11214