Changes in the Transcription of Proliferation- and Apoptosis-Related Genes in Embryos in Women of Different Ages under the Influence of Extracellular Vesicles from Donor Follicular Fluid In Vitro.

التفاصيل البيبلوغرافية
العنوان:	Changes in the Transcription of Proliferation- and Apoptosis-Related Genes in Embryos in Women of Different Ages under the Influence of Extracellular Vesicles from Donor Follicular Fluid In Vitro.
المؤلفون:	Nepsha OS; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia. o_nepsha@oparina4.ru., Burmenskaya OV; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia., Akhmedova ZF; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia., Romanov EA; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia., Sysoeva AP; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia., Goryunov KV; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia., Shevtsova YA; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia., Silachev DN; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia., Makarova NP; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia., Kalinina EA; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
المصدر:	Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2024 Mar; Vol. 176 (5), pp. 658-665. Date of Electronic Publication: 2024 May 10.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Springer Country of Publication: United States NLM ID: 0372557 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-8221 (Electronic) Linking ISSN: 00074888 NLM ISO Abbreviation: Bull Exp Biol Med Subsets: MEDLINE
أسماء مطبوعة:	Publication: New York : Springer Original Publication: New York, Consultants Bureau.
مواضيع طبية MeSH:	Extracellular Vesicles/metabolism , Extracellular Vesicles/genetics , Apoptosis/genetics , Follicular Fluid/metabolism , Blastocyst/metabolism , Blastocyst/cytology, Humans ; Female ; Adult ; Gene Expression Regulation, Developmental ; Cell Proliferation ; Oocytes/metabolism ; Age Factors ; Embryonic Development/genetics ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
مستخلص:	We studied the influence of extracellular vesicles from the follicular fluid of a young donor on gene expression (MKI67, MYBL2, CCNB1, CCND1, CCNE1, CALM2, BAX, NDRG1, TP53I3, VEGF, VCAN, HAS2, CTSL2, PIBF1, RPL37, PFKP, GPX3, and AQP3) in embryos of women of different ages. According to nanoparticle tracking analysis data, the concentration of extracellular vesicles was 3.75±0.47×10 ¹¹ particles/ml and the mean particle size was 138.78±9.90 nm. During co-culturing of the follicular fluid extracellular vesicles with blastocysts of young women, we observed significantly increased expression of mRNA for genes CTSL2, CCND1, CCNE1, VEGF and reduced expression of BAX gene mRNA in comparison with embryos in women of late reproductive age. We hypothesized that addition of extracellular vesicles of the oocyte follicular fluid from a young donor to the culture medium of embryos could slow down apoptosis process typical of blastocyst cells in women above 36 years. (© 2024. Springer Science+Business Media, LLC, part of Springer Nature.)
References:	Wang X, Wang L, Xiang W. Mechanisms of ovarian aging in women: a review. J. Ovarian Res. 2023;16(1):67. doi: https://doi.org/10.1186/s13048-023-01151-z. (PMID: 10.1186/s13048-023-01151-z3702497610080932) Fan W, Qi Y, Wang Y, Yan H, Li X, Zhang Y. Messenger roles of extracellular vesicles during fertilization of gametes, development and implantation: Recent advances. Front. Cell Dev. Biol. 2023;10:1079387. doi: https://doi.org/10.3389/fcell.2022.1079387. (PMID: 10.3389/fcell.2022.1079387366844319849778) Manni G, Buratta S, Pallotta MT, Chiasserini D, Di Michele A, Emiliani C, Giovagnoli S, Pascucci L, Romani R, Bellezza I, Urbanelli L, Fallarino F. Extracellular vesicles in aging: an emerging hallmark? Cells. 2023;12(4):527. doi: https://doi.org/10.3390/cells12040527. (PMID: 10.3390/cells12040527368311949954704) Sysoeva AP, Makarova NP, Silachev DN, Lobanova NN, Shevtsova YA, Bragina EE, Kalinina EA, Sukhikh GT. Influence of extracellular vesicles of the follicular fluid on morphofunctional characteristics of human sperm. Bull. Exp. Biol. Med. 2021;172(2):254-262. doi: https://doi.org/10.1007/s10517-021-05372-4. (PMID: 10.1007/s10517-021-05372-434855079) Sysoeva AP, Nepsha OS, Makarova NP, Silachev DN, Lobanova NN, Timofeeva AV, Shevtsova YA, Bragina EE, Kalinina EA. Influence of extracellular vesicles from the follicular fluid of young women and women of advanced maternal age with different miRNA profiles on sperm functional properties. Bull. Exp. Biol. Med. 2022;173(4):560-568. doi: https://doi.org/10.1007/s10517-022-05589-x. (PMID: 10.1007/s10517-022-05589-x36094592) Gabryś J, Kij-Mitka B, Sawicki S, Kochan J, Nowak A, Łojko J, Karnas E, Bugno-Poniewierska M. Extracellular vesicles from follicular fluid may improve the nuclear maturation rate of in vitro matured mare oocytes. Theriogenology. 2022;188:116-124. doi: https://doi.org/10.1016/j.theriogenology.2022.05.022. (PMID: 10.1016/j.theriogenology.2022.05.02235689941) da Silveira JC, Andrade GM, Del Collado M, Sampaio RV, Sangalli JR, Silva LA, Pinaffi FVL, Jardim IB, Cesar MC, Nogueira MFG, Cesar ASM, Coutinho LL, Pereira RW, Perecin F, Meirelles FV. Supplementation with small-extracellular vesicles from ovarian follicular fluid during in vitro production modulates bovine embryo development. PLoS One. 2017;12(6):e0179451. doi: https://doi.org/10.1371/journal.pone.0179451. (PMID: 10.1371/journal.pone.0179451286178215472319) Kenigsberg S, Wyse BA, Librach CL, da Silveira JC. Protocol for exosome isolation from small volume of ovarian follicular fluid: evaluation of ultracentrifugation and commercial kits. Methods Mol. Biol. 2017;1660:321-341. doi: https://doi.org/10.1007/978-1-4939-7253-1_26. (PMID: 10.1007/978-1-4939-7253-1_2628828668) Soares M, Pinto MM, Nobre RJ, de Almeida LP, da Graça Rasteiro M, Almeida-Santos T, Ramalho-Santos J, Sousa AP. Isolation of extracellular vesicles from human follicular fluid: size-exclusion chromatography versus ultracentrifugation. Biomolecules. 2023;13(2):278. doi: https://doi.org/10.3390/biom13020278. (PMID: 10.3390/biom13020278368306479953485) Lim S, Kaldis P. Cdks, cyclins and CKIs: roles beyond cell cycle regulation. Development. 2013;140(15):3079-3093. doi: https://doi.org/10.1242/dev.091744. (PMID: 10.1242/dev.09174423861057) Raes A, Wydooghe E, Pavani KC, Bogado Pascottini O, Van Steendam K, Dhaenens M, Boel A, Heras S, Heindryckx B, Peelman L, Deforce D, Van Nieuwerburgh F, Opsomer G, Van Soom A, Smits K. Cathepsin-L secreted by high-quality bovine embryos exerts an embryotrophic effect in vitro. Int. J. Mol. Sci. 2023;24(7):6563. doi: https://doi.org/10.3390/ijms24076563. (PMID: 10.3390/ijms240765633704753510094850) Khurana P, Smyth NR, Sheth B, Velazquez MA, Eckert JJ, Fleming TP. Advanced maternal age perturbs mouse embryo development and alters the phenotype of derived embryonic stem cells. J. Dev. Orig. Health Dis. 2022;13(3):395-405. doi: https://doi.org/10.1017/S2040174421000325. (PMID: 10.1017/S204017442100032534193331) Kawai K, Harada T, Ishikawa T, Sugiyama R, Kawamura T, Yoshida A, Tsutsumi O, Ishino F, Kubota T, Kohda T. Parental age and gene expression profiles in individual human blastocysts. Sci. Rep. 2018;8(1):2380. doi: https://doi.org/10.1038/s41598-018-20614-8. (PMID: 10.1038/s41598-018-20614-8294029205799158) McCallie BR, Parks JC, Trahan GD, Jones KL, Coate BD, Griffin DK, Schoolcraft WB, Katz-Jaffe MG. Compromised global embryonic transcriptome associated with advanced maternal age. J. Assist. Reprod. Genet. 2019;36(5):915-924. doi: https://doi.org/10.1007/s10815-019-01438-5. (PMID: 10.1007/s10815-019-01438-5310251586541584) Ezoe K, Miki T, Akaike H, Shimazaki K, Takahashi T, Tanimura Y, Amagai A, Sawado A, Mogi M, Kaneko S, Ueno S, Coticchio G, Cimadomo D, Borini A, Rienzi L, Kato K. Maternal age affects pronuclear and chromatin dynamics, morula compaction and cell polarity, and blastulation of human embryos. Hum. Reprod. 2023;38(3):387-399. doi: https://doi.org/10.1093/humrep/dead001. (PMID: 10.1093/humrep/dead00136644923) Chaudhry SR, Lopes J, Levin NK, Kalpage H, Tainsky MA. Germline mutations in apoptosis pathway genes in ovarian cancer; the functional role of a TP53I3 (PIG3) variant in ROS production and DNA repair. Cell Death Discov. 2021;7(1):62. doi: https://doi.org/10.1038/s41420-021-00442-y. (PMID: 10.1038/s41420-021-00442-y337823978007802) Jiang XD, Liu Y, Wu JF, Gong SN, Ma Y, Zi XD. Regulation of proliferation, apoptosis, hormone secretion and gene expression by acetyl-L-carnitine in yak (Bos grunniens) granulosa cells. Theriogenology. 2023;203:61-68. doi: https://doi.org/10.1016/j.theriogenology.2023.03.016. (PMID: 10.1016/j.theriogenology.2023.03.01636972666) Diez-Fraile A, Lammens T, Tilleman K, Witkowski W, Verhasselt B, De Sutter P, Benoit Y, Espeel M, D’Herde K. Age-associated differential microRNA levels in human follicular fluid reveal pathways potentially determining fertility and success of in vitro fertilization. Hum. Fertil. (Camb). 2014;17(2):90-98. doi: https://doi.org/10.3109/14647273.2014.897006. (PMID: 10.3109/14647273.2014.89700624684237) Battaglia R, Musumeci P, Ragusa M, Barbagallo D, Scalia M, Zimbone M, Lo Faro JM, Borzì P, Scollo P, Purrello M, Vento EM, Di Pietro C. Ovarian aging increases small extracellular vesicle CD81+ release in human follicular fluid and influences miRNA profiles. Aging (Albany NY). 2020;12(12):12 324-12 341. doi: https://doi.org/10.18632/aging.103441. Fang X, Tanga BM, Bang S, Seo C, Kim H, Saadeldin IM, Lee S, Cho J. Oviduct epithelial cell-derived extracellular vesicles improve porcine trophoblast outgrowth. Vet. Sci. 2022;9(11):609. doi: https://doi.org/10.3390/vetsci9110609. (PMID: 10.3390/vetsci9110609363560869697688) Qu P, Luo S, Du Y, Zhang Y, Song X, Yuan X, Lin Z, Li Y, Liu E. Extracellular vesicles and melatonin benefit embryonic develop by regulating reactive oxygen species and 5-methylcytosine. J. Pineal Res. 2020;68(3):e12635. doi: https://doi.org/10.1111/jpi.12635. (PMID: 10.1111/jpi.12635320123547154726) Tsukazawa KS, Li L, Tse WKF. 2,4-dichlorophenol exposure induces lipid accumulation and reactive oxygen species formation in zebrafish embryos. Ecotoxicol. Environ. Saf. 2022;230:113133. doi: https://doi.org/10.1016/j.ecoenv.2021.113133. (PMID: 10.1016/j.ecoenv.2021.11313334971995) Qu P, Zhao Y, Wang R, Zhang Y, Li L, Fan J, Liu E. Extracellular vesicles derived from donor oviduct fluid improved birth rates after embryo transfer in mice. Reprod. Fertil. Dev. 2019;31(2):324-332. doi: https://doi.org/10.1071/RD18203. (PMID: 10.1071/RD1820330196804) Sidrat T, Khan AA, Joo MD, Wei Y, Lee KL, Xu L, Kong IK. Bovine oviduct epithelial cell-derived culture media and exosomes improve mitochondrial health by restoring metabolic flux during pre-implantation development. Int. J. Mol. Sci. 2020;21(20):7589. doi: https://doi.org/10.3390/ijms21207589. (PMID: 10.3390/ijms21207589330665627593913) Fang X, Bang S, Tanga BM, Seo C, Zhou D, Seong G, Saadeldin IM, Lee S, Cui XS, Cho J. Oviduct epithelial cell-derived extracellular vesicles promote the developmental competence of IVF porcine embryos. Mol. Med. Rep. 2023;27(6):122. doi: https://doi.org/10.3892/mmr.2023.13009. (PMID: 10.3892/mmr.2023.130093720339110206610)
فهرسة مساهمة:	Keywords: co-culturing; embryo; extracellular vesicles; follicular fluid; mRNA
تواريخ الأحداث:	Date Created: 20240510 Date Completed: 20240517 Latest Revision: 20240517
رمز التحديث:	20240517
DOI:	10.1007/s10517-024-06087-y
PMID:	38727955
قاعدة البيانات:	MEDLINE

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تدمد:	1573-8221
DOI:	10.1007/s10517-024-06087-y