دورية أكاديمية
Molecular characteristics of Echinococcus multilocularis FABP1 and its regulatory functions on murine macrophages.
| العنوان: | Molecular characteristics of Echinococcus multilocularis FABP1 and its regulatory functions on murine macrophages. |
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| المؤلفون: | Li X; Department of Pathogen Biology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China., Liao P; Department of Pathogen Biology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China., Zhou W; Department of Pathogen Biology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China., Yang X; Department of Pathogen Biology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China., Ye B; Department of Pathogen Biology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China. Electronic address: yebin@cqmu.edu.cn. |
| المصدر: | Acta tropica [Acta Trop] 2024 Jul; Vol. 255, pp. 107247. Date of Electronic Publication: 2024 May 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6254 (Electronic) Linking ISSN: 0001706X NLM ISO Abbreviation: Acta Trop Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Basel. |
| مواضيع طبية MeSH: | Echinococcus multilocularis*/genetics , Echinococcus multilocularis*/immunology , Macrophages*/immunology , Macrophages*/parasitology , Fatty Acid-Binding Proteins*/genetics , Fatty Acid-Binding Proteins*/metabolism , Helminth Proteins*/genetics , Helminth Proteins*/metabolism , Helminth Proteins*/immunology , Phagocytosis*, Animals ; Mice ; Nitric Oxide/metabolism ; Apoptosis ; Cytokines/metabolism ; RAW 264.7 Cells |
| مستخلص: | Fatty acid binding proteins (FABPs) have emerged as attractive vaccination candidates for several platyhelminth species. To explore the physiological functions of Echinococcus multilocularis (E. multilocularis) FABP, the molecular characteristics of EmFABP1 were analyzed by online software, and the regulatory roles of rEmFABP1 protein in murine macrophages were further investigated. The emfabp1 gene encodes 133 amino acids with the characteristic β-barrel shape of the cytoplasmic FABP family. Natural EmFABP1 protein is predominantly expressed in protoscoleces tegument and germinal layer cells and is also detected in cyst fluid and exosomes of E. multilocularis. rEmFABP1 protein demonstrated a notable suppression of phagocytic activity and nitric oxide production in murine macrophages. Additionally, the protein was observed to promote apoptosis and regulate cytokine expression in macrophages. These findings suggested that E. multilocularis FABP1 is critical in modifying macrophage physiological processes and that this protein may have immunomodulatory roles during infection. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Echinococcus multilocularis; Fatty acid binding proteins; Immunomodulatory; Murine macrophages; Organizational localization |
| المشرفين على المادة: | 0 (Fatty Acid-Binding Proteins) 0 (Helminth Proteins) 31C4KY9ESH (Nitric Oxide) 0 (Cytokines) |
| تواريخ الأحداث: | Date Created: 20240510 Date Completed: 20240524 Latest Revision: 20240608 |
| رمز التحديث: | 20240608 |
| DOI: | 10.1016/j.actatropica.2024.107247 |
| PMID: | 38729330 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6254 |
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| DOI: | 10.1016/j.actatropica.2024.107247 |