دورية أكاديمية
Hydrogel/microcarrier cell scaffolds for rapid expansion of satellite cells from large yellow croakers: Differential analysis between 2D and 3D cell culture.
| العنوان: | Hydrogel/microcarrier cell scaffolds for rapid expansion of satellite cells from large yellow croakers: Differential analysis between 2D and 3D cell culture. |
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| المؤلفون: | Yin H; State Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China; Qingdao Institute of Marine Bioresources for Nutrition & Health Innovation, Qingdao 266109, PR China., Zhou X; State Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China; Qingdao Institute of Marine Bioresources for Nutrition & Health Innovation, Qingdao 266109, PR China., Jin Hur S; Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea., Liu H; Qingdao Institute of Marine Bioresources for Nutrition & Health Innovation, Qingdao 266109, PR China., Zheng H; State Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China; Qingdao Institute of Marine Bioresources for Nutrition & Health Innovation, Qingdao 266109, PR China. Electronic address: zhenghongwei@ouc.edu.cn., Xue C; State Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China; Qingdao Institute of Marine Bioresources for Nutrition & Health Innovation, Qingdao 266109, PR China. Electronic address: xuech@ouc.edu.cn. |
| المصدر: | Food research international (Ottawa, Ont.) [Food Res Int] 2024 Jun; Vol. 186, pp. 114396. Date of Electronic Publication: 2024 Apr 21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science Country of Publication: Canada NLM ID: 9210143 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7145 (Electronic) Linking ISSN: 09639969 NLM ISO Abbreviation: Food Res Int Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992- |
| مواضيع طبية MeSH: | Satellite Cells, Skeletal Muscle*/metabolism , Satellite Cells, Skeletal Muscle*/cytology , Hydrogels*/chemistry , Cell Proliferation* , Cell Culture Techniques* , Cell Differentiation* , Tissue Scaffolds*/chemistry , Cell Adhesion*, Animals ; Cell Culture Techniques, Three Dimensional/methods ; Cells, Cultured ; Desmin/metabolism ; PAX7 Transcription Factor/metabolism ; PAX7 Transcription Factor/genetics ; Muscle Development |
| مستخلص: | Cell culture meat is based on the scaled-up expansion of seed cells. The biological differences between seed cells from large yellow croakers in the two-dimensional (2D) and three-dimensional (3D) culture systems have not been explored. Here, satellite cells (SCs) from large yellow croakers (Larimichthys crocea) were grown on cell climbing slices, hydrogels, and microcarriers for five days to analyze the biological differences of SCs on different cell scaffolds. The results exhibited that SCs had different cell morphologies in 2D and 3D cultures. Cell adhesion receptors (Itgb1andsdc4) and adhesion spot markervclof the 3D cultures were markedly expressed. Furthermore, myogenic decision markers (Pax7andmyod) were significantly enhanced. However, the expression of myogenic differentiation marker (desmin) was significantly increased in the microcarrier group. Combined with the transcriptome data, this suggests that cell adhesion of SCs in 3D culture was related to the integrin signaling pathway. In contrast, the slight spontaneous differentiation of SCs on microcarriers was associated with rapid cell proliferation. This study is the first to report the biological differences between SCs in 2D and 3D cultures, providing new perspectives for the rapid expansion of cell culture meat-seeded cells and the development of customized scaffolds. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Hydrogels; Large yellow croakers; Microcarriers; Satellite cells; Three-dimensional cell culture; Two-dimensional cell culture |
| المشرفين على المادة: | 0 (Hydrogels) 0 (Desmin) 0 (PAX7 Transcription Factor) |
| تواريخ الأحداث: | Date Created: 20240510 Date Completed: 20240510 Latest Revision: 20240510 |
| رمز التحديث: | 20240511 |
| DOI: | 10.1016/j.foodres.2024.114396 |
| PMID: | 38729738 |
| قاعدة البيانات: | MEDLINE |
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