دورية أكاديمية
Peripheral blood transcriptomic profiling of molecular mechanisms commonly regulated by binge drinking and placebo effects.
العنوان: | Peripheral blood transcriptomic profiling of molecular mechanisms commonly regulated by binge drinking and placebo effects. |
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المؤلفون: | Shetty AC; Institute for Genome Sciences, University of Maryland School of Medicine, 670 W. Baltimore Street, Baltimore, MD, 21201, USA., Sivinski J; Institute for Genome Sciences, University of Maryland School of Medicine, 670 W. Baltimore Street, Baltimore, MD, 21201, USA., Cornell J; Institute for Genome Sciences, University of Maryland School of Medicine, 670 W. Baltimore Street, Baltimore, MD, 21201, USA., McCracken C; Institute for Genome Sciences, University of Maryland School of Medicine, 670 W. Baltimore Street, Baltimore, MD, 21201, USA., Sadzewicz L; Institute for Genome Sciences, University of Maryland School of Medicine, 670 W. Baltimore Street, Baltimore, MD, 21201, USA., Mahurkar A; Institute for Genome Sciences, University of Maryland School of Medicine, 670 W. Baltimore Street, Baltimore, MD, 21201, USA., Wang XQ; Department of Public Health Sciences, University of Virginia, Charlottesville, VA, USA., Colloca L; Department of Pain and Translational Symptom Science, Placebo Beyond Opinions (PBO) Center, University of Maryland School of Nursing, Baltimore, MD, USA., Lin W; Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, MD, USA., Pilli N; Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD, USA., Kane MA; Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD, USA., Seneviratne C; Institute for Genome Sciences, University of Maryland School of Medicine, 670 W. Baltimore Street, Baltimore, MD, 21201, USA. chamindi.seneviratne@nih.gov.; Department of Pharmacology, University of Maryland School of Medicine, Baltimore, MD, USA. chamindi.seneviratne@nih.gov. |
المصدر: | Scientific reports [Sci Rep] 2024 May 10; Vol. 14 (1), pp. 10733. Date of Electronic Publication: 2024 May 10. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
مواضيع طبية MeSH: | Binge Drinking*/blood , Binge Drinking*/genetics , Placebo Effect* , Transcriptome* , Gene Expression Profiling*, Humans ; Male ; Female ; Adult ; Young Adult ; Ethanol ; Longitudinal Studies ; Gene Expression Regulation/drug effects |
مستخلص: | Molecular responses to alcohol consumption are dynamic, context-dependent, and arise from a complex interplay of biological and external factors. While many have studied genetic risk associated with drinking patterns, comprehensive studies identifying dynamic responses to pharmacologic and psychological/placebo effects underlying binge drinking are lacking. We investigated transcriptome-wide response to binge, medium, and placebo alcohol consumption by 17 healthy heavy social drinkers enrolled in a controlled, in-house, longitudinal study of up to 12 days. Using RNA-seq, we identified 251 and 13 differentially expressed genes (DEGs) in response to binge drinking and placebo, respectively. Eleven protein-coding DEGs had very large effect sizes in response to binge drinking (Cohen's d > 1). Furthermore, binge dose significantly impacted the Cytokine-cytokine receptor interaction pathway (KEGG: hsa04060) across all experimental sequences. Placebo also impacted hsa04060, but only when administered following regular alcohol drinking sessions. Similarly, medium-dose and placebo commonly impacted KEGG pathways of Systemic lupus erythematosus, Neutrophil extracellular trap formation, and Alcoholism based on the sequence of drinking sessions. These findings together indicate the "dose-extending effects" of placebo at a molecular level. Furthermore, besides supporting alcohol dose-specific molecular changes, results suggest that the placebo effects may induce molecular responses within the same pathways regulated by alcohol. (© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.) |
التعليقات: | Update of: medRxiv. 2023 Mar 24;:. (PMID: 36993621) |
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معلومات مُعتمدة: | K23 AA020899 United States AA NIAAA NIH HHS; UL1 TR003098 United States TR NCATS NIH HHS; R01 AA026291 United States AA NIAAA NIH HHS |
المشرفين على المادة: | 3K9958V90M (Ethanol) |
تواريخ الأحداث: | Date Created: 20240510 Date Completed: 20240510 Latest Revision: 20240520 |
رمز التحديث: | 20240520 |
مُعرف محوري في PubMed: | PMC11087488 |
DOI: | 10.1038/s41598-024-56900-x |
PMID: | 38730024 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2045-2322 |
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DOI: | 10.1038/s41598-024-56900-x |