دورية أكاديمية
Modulation of the microRNA-6089/E2F transcription factor2 axis by querceting: implications for osteoblast viability, proliferation, migration, and osteogenic differentiation in fracture healing.
| العنوان: | Modulation of the microRNA-6089/E2F transcription factor2 axis by querceting: implications for osteoblast viability, proliferation, migration, and osteogenic differentiation in fracture healing. |
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| المؤلفون: | Dong R; Department of Spine Surgery, Anhui Wanbei Coal-Electricity Group General Hospital, Suzhou City, Anhui Province, China., Liu MY; Fourth Central Clinical School, Tianjin Medical University, Tianjin City, China., Zhu GB; General Clinical Research Center, Anhui Wanbei Coal-Electricity Group General Hospital, Suzhou City, Anhui Province, China., Tan KM; General Clinical Research Center, Anhui Wanbei Coal-Electricity Group General Hospital, Suzhou City, Anhui Province, China., Wang YQ; Department of Orthopedic Surgery, Tianjin Fourth Central Hospital, Tianjin Medical University, Tianjin, China. yongqing_wang139@hotmail.com., Li L; General Clinical Research Center, Anhui Wanbei Coal-Electricity Group General Hospital, Suzhou City, Anhui Province, China. lilili07@163.com. |
| المصدر: | Journal of physiology and pharmacology : an official journal of the Polish Physiological Society [J Physiol Pharmacol] 2024 Apr; Vol. 75 (2), pp. 173-183. Date of Electronic Publication: 2024 May 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Polish Physiological Society Country of Publication: Poland NLM ID: 9114501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1899-1505 (Electronic) Linking ISSN: 08675910 NLM ISO Abbreviation: J Physiol Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Krakow : Polish Physiological Society Original Publication: [Kraków] : The Society, [1991- |
| مواضيع طبية MeSH: | MicroRNAs*/genetics , MicroRNAs*/metabolism , Osteoblasts*/drug effects , Osteoblasts*/metabolism , Fracture Healing*/drug effects , Cell Proliferation*/drug effects , Osteogenesis*/drug effects , Cell Movement*/drug effects , Cell Differentiation*/drug effects , Cell Survival*/drug effects , Quercetin*/pharmacology , E2F2 Transcription Factor*/metabolism , E2F2 Transcription Factor*/genetics, Animals ; Mice ; Male ; Cell Line ; Apoptosis/drug effects |
| مستخلص: | Quercetin is widely distributed in plants as a flavonol compound with multiple biological activities. It has been found that quercetin can regulate bone homeostasis through multiple pathways and targets. This study investigated the role and specific molecular mechanisms of quercetin in regulating osteoblast viability, proliferation, migration and osteogenic differentiation. A mouse model of traumatic fracture was established and then 100 mg/kg quercetin corn oil suspension was gavaged at the same time every day for 28 days. miR-6089 and E2F transcription factor 2 (E2F2) expression levels in mice were measured. Fracture healing in mice was observed. MC3T3-E1 cells were transfected with plasmids targeting miR-6089 and E2F2, and cell viability, proliferation, migration, apoptosis, and osteogenic differentiation were determined. The targeting relationship between miR-6089 and E2F2 was verified. In vivo experiments showed that quercetin significantly increased osteocalcin (OCN) expression (P<0.05) and promoted fracture healing in traumatic fracture (TF) mice. miR-6089 expression was down-regulated (P<0.05) and E2F2 expression was up-regulated (P<0.05) in TF mice. Quercetin promoted miR-6089 expression and inhibited E2F2 expression (both P<0.05). In vitro results showed that quercetin promoted miR-6089 expression and inhibited E2F2 expression in a dose-dependent manner (both P<0.05). Quercetin dose-dependently promoted MC3T3-E1 cell viability, proliferation, migration, and osteogenic differentiation, and inhibited MC3T3-E1 cell apoptosis (all P<0.05). Up-regulating miR-6089 further promoted MC3T3-E1 cell viability, proliferation, migration and osteogenic differentiation, and inhibited MC3T3-E1 cell apoptosis (all P<0.05). miR-6089 targeted and regulated E2F2 expression. Up-regulating E2F2 attenuated the promoting effect of up-regulated miR-6089 on MC3T3-E1 cell viability, proliferation, migration, osteogenic differentiation, and inhibition of apoptosis (all P<0.05). We conclude that quercetin enhances osteoblast viability, proliferation, migration, and osteogenic differentiation by modulating the miR-6089/E2F2 axis, thereby promoting fracture healing. |
| المشرفين على المادة: | 0 (MicroRNAs) 9IKM0I5T1E (Quercetin) 0 (E2F2 Transcription Factor) |
| تواريخ الأحداث: | Date Created: 20240513 Date Completed: 20240513 Latest Revision: 20240513 |
| رمز التحديث: | 20240513 |
| DOI: | 10.26402/jpp.2024.2.06 |
| PMID: | 38736264 |
| قاعدة البيانات: | MEDLINE |
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