دورية أكاديمية
Identification and fine mapping of Brmmd1 gene controlling recessive genic male sterility in Brassica rapa L.
| العنوان: | Identification and fine mapping of Brmmd1 gene controlling recessive genic male sterility in Brassica rapa L. |
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| المؤلفون: | Xiong X; College of Bioengineering, Jingchu University of Technology, Jingmen 448000, People's Republic of China., Li X; Key Laboratory of Vegetable Ecological Cultivation On Highland, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement, Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan 430063, People's Republic of China., Zhang S; Key Laboratory of Vegetable Ecological Cultivation On Highland, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement, Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan 430063, People's Republic of China., Hu Z; Key Laboratory of Vegetable Ecological Cultivation On Highland, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement, Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan 430063, People's Republic of China., Liu T; Zhejiang Institute of Landscape Plants and Flowers, Hangzhou 311251, People's Republic of China., Qiu Z; Key Laboratory of Vegetable Ecological Cultivation On Highland, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement, Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan 430063, People's Republic of China., Cao J; Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, People's Republic of China., Huang L; Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, People's Republic of China. Electronic address: lihuang@zju.edu.cn., Yan C; Key Laboratory of Vegetable Ecological Cultivation On Highland, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement, Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan 430063, People's Republic of China. Electronic address: yanch@hbaas.ac.cn. |
| المصدر: | Gene [Gene] 2024 Oct 05; Vol. 924, pp. 148558. Date of Electronic Publication: 2024 May 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland, 1976- |
| مواضيع طبية MeSH: | Brassica rapa*/genetics , Brassica rapa*/metabolism , Genes, Recessive* , Plant Infertility*/genetics , Plant Proteins*/genetics , Plant Proteins*/metabolism, Chromosome Mapping ; Genes, Plant ; Genetic Linkage ; Mutation |
| مستخلص: | Recessive genic male sterility (RGMS) provides an effective approach for the commercial exploitation of heterosis, especially in Brassica crops. Although some artificial RGMS mutants have been reported in B. rapa, no causal genes derived from these natural mutants have been identified so far. In this study, a spontaneous RGMS mutant Bcajh97-01A derived from the 'Aijiaohuang' line traced back to the 1980 s was identified. Genetic analysis revealed that the RGMS trait was controlled by a single locus in the Bcajh97-01A/B system. Bulk segregant analysis (BSA) in combination with linkage analysis was employed to delimit the causal gene to an approximate 129 kb interval on chromosome A02. The integrated information of transcriptional levels and the predicted genes in the target region indicated that the Brmmd1 (BraA02g017420) encoding a PHD-containing nuclear protein was the most likely candidate gene. A 374 bp miniature inverted-repeat transposable element (MITE) was inserted into the first exon to prematurely stop the Brmmd1 gene translation, thus blocking the normal expression of this gene at the tetrad stage in the Bcajh97-01A. Additionally, a co-segregating structure variation (SV) marker was developed to rapidly screen the RGMS progenies from Bcajh97-01A/B system. Our findings reveal that BraA02g017420 is the causal gene responsible for the RGMS trait. This study lays a foundation for marker-assisted selection and further molecular mechanism exploration of pollen development in B. rapa. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Brassica rapa; Brmmd1; Fine mapping; Genic male sterility |
| المشرفين على المادة: | 0 (Plant Proteins) |
| تواريخ الأحداث: | Date Created: 20240513 Date Completed: 20240614 Latest Revision: 20240716 |
| رمز التحديث: | 20240716 |
| DOI: | 10.1016/j.gene.2024.148558 |
| PMID: | 38740353 |
| قاعدة البيانات: | MEDLINE |
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