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Cyp6g2 is the major P450 epoxidase responsible for juvenile hormone biosynthesis in Drosophila melanogaster.

التفاصيل البيبلوغرافية
العنوان: Cyp6g2 is the major P450 epoxidase responsible for juvenile hormone biosynthesis in Drosophila melanogaster.
المؤلفون: Jia Q; Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou, China.; Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou, China., Yang L; Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou, China., Wen J; Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou, China., Liu S; Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou, China.; Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou, China., Wen D; College of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyuan, 558000, China., Luo W; Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou, China.; Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou, China., Wang W; Center of Pharmaceutical Technology, Tsinghua University, Beijing, 100084, China., Palli SR; Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY, 40546, USA., Sheng L; Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou, China. lisheng@scnu.edu.cn.; Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou, China. lisheng@scnu.edu.cn.
المصدر: BMC biology [BMC Biol] 2024 May 13; Vol. 22 (1), pp. 111. Date of Electronic Publication: 2024 May 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101190720 Publication Model: Electronic Cited Medium: Internet ISSN: 1741-7007 (Electronic) Linking ISSN: 17417007 NLM ISO Abbreviation: BMC Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : BioMed Central, c2003-
مواضيع طبية MeSH: Cytochrome P-450 Enzyme System*/genetics , Cytochrome P-450 Enzyme System*/metabolism , Drosophila melanogaster*/genetics , Drosophila melanogaster*/growth & development , Juvenile Hormones*/biosynthesis , Juvenile Hormones*/metabolism, Animals ; Corpora Allata/metabolism ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Larva/growth & development ; Larva/genetics ; Metamorphosis, Biological/genetics ; Oxidoreductases ; Pupa/growth & development ; Pupa/genetics ; Pupa/metabolism
مستخلص: Background: Juvenile hormones (JH) play crucial role in regulating development and reproduction in insects. The most common form of JH is JH III, derived from MF through epoxidation by CYP15 enzymes. However, in the higher dipterans, such as the fruitfly, Drosophila melanogaster, a bis-epoxide form of JHB3, accounted most of the JH detected. Moreover, these higher dipterans have lost the CYP15 gene from their genomes. As a result, the identity of the P450 epoxidase in the JH biosynthesis pathway in higher dipterans remains unknown.
Results: In this study, we show that Cyp6g2 serves as the major JH epoxidase responsible for the biosynthesis of JHB3 and JH III in D. melanogaster. The Cyp6g2 is predominantly expressed in the corpus allatum (CA), concurring with the expression pattern of jhamt, another well-studied gene that is crucial in the last steps of JH biosynthesis. Mutation in Cyp6g2 leads to severe disruptions in larval-pupal metamorphosis and exhibits reproductive deficiencies, exceeding those seen in jhamt mutants. Notably, Cyp6g2 -/- ::jhamt 2 double mutants all died at the pupal stage but could be rescued through the topical application of JH analogs. JH titer analyses revealed that both Cyp6g2 -/- mutant and jhamt 2 mutant lacking JHB3 and JH III, while overexpression of Cyp6g2 or jhamt caused a significant increase in JHB3 and JH III titer.
Conclusions: These findings collectively established that Cyp6g2 as the major JH epoxidase in the higher dipterans and laid the groundwork for the further understanding of JH biosynthesis. Moreover, these findings pave the way for developing specific Cyp6g2 inhibitors as insect growth regulators or insecticides.
(© 2024. The Author(s).)
References: Biotechnol Appl Biochem. 2013 Jan-Feb;60(1):82-91. (PMID: 23586995)
Proc Natl Acad Sci U S A. 2015 Aug 4;112(31):E4226-35. (PMID: 26195792)
Insects. 2020 Dec 03;11(12):. (PMID: 33287115)
Arch Insect Biochem Physiol. 1995;30(2-3):225-37. (PMID: 7579573)
Insect Biochem Mol Biol. 2020 Jan;116:103287. (PMID: 31760138)
Insect Biochem Mol Biol. 2010 Dec;40(12):891-7. (PMID: 20849954)
Insect Biochem Mol Biol. 2019 Oct;113:103210. (PMID: 31422152)
Insect Biochem Mol Biol. 2008 Jul;38(7):714-20. (PMID: 18549957)
Insect Biochem Mol Biol. 2020 Dec;127:103490. (PMID: 33169702)
PLoS Genet. 2022 Jun 27;18(6):e1010292. (PMID: 35759519)
Int J Mol Sci. 2022 May 26;23(11):. (PMID: 35682678)
Curr Biol. 2024 Feb 5;34(3):505-518.e6. (PMID: 38215744)
Development. 2011 Jun;138(11):2283-91. (PMID: 21558376)
Development. 2020 Oct 23;147(20):. (PMID: 33097549)
J Biol Chem. 2017 Dec 29;292(52):21504-21516. (PMID: 29118190)
BMC Biol. 2021 Oct 8;19(1):222. (PMID: 34625063)
G3 (Bethesda). 2017 Feb 9;7(2):467-479. (PMID: 27974438)
Proc Natl Acad Sci U S A. 2018 Jan 2;115(1):139-144. (PMID: 29255055)
Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5731-6. (PMID: 19289821)
Sci Rep. 2017 Sep 12;7(1):11338. (PMID: 28900129)
PLoS Genet. 2015 Jul 10;11(7):e1005394. (PMID: 26161662)
Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):11986-91. (PMID: 14530389)
FEBS J. 2008 Jun;275(11):2919-31. (PMID: 18435763)
Chem Res Toxicol. 1997 Feb;10(2):156-64. (PMID: 9049426)
Proc Natl Acad Sci U S A. 1989 Feb;86(4):1421-5. (PMID: 2493154)
J Insect Physiol. 2023 Mar;145:104487. (PMID: 36707023)
J Insect Physiol. 2003 Jun;49(6):603-9. (PMID: 12804720)
Insect Biochem Mol Biol. 2007 May;37(5):512-9. (PMID: 17456446)
Sci Bull (Beijing). 2022 Jan 30;67(2):186-197. (PMID: 36546012)
BMC Biol. 2021 Feb 25;19(1):39. (PMID: 33632227)
Proc Natl Acad Sci U S A. 2016 May 17;113(20):5634-9. (PMID: 27140602)
Development. 2009 Jun;136(12):2015-25. (PMID: 19465595)
Insect Biochem Mol Biol. 2011 Apr;41(4):228-35. (PMID: 21195763)
Development. 2023 May 15;150(10):. (PMID: 37218457)
Proc Natl Acad Sci U S A. 2021 Nov 9;118(45):. (PMID: 34697248)
Gen Comp Endocrinol. 2011 May 15;172(1):56-61. (PMID: 21354154)
Insect Biochem Mol Biol. 2011 Apr;41(4):219-27. (PMID: 21195178)
PLoS Genet. 2015 Mar 16;11(3):e1005038. (PMID: 25774983)
BMC Biol. 2022 Dec 13;20(1):278. (PMID: 36514097)
Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):E1871-9. (PMID: 25825754)
Development. 2010 Apr;137(7):1117-26. (PMID: 20181742)
J Insect Physiol. 2015 Sep;80:61-70. (PMID: 25921675)
PLoS Genet. 2012;8(3):e1002486. (PMID: 22412378)
Annu Rev Entomol. 2013;58:181-204. (PMID: 22994547)
Insect Biochem Mol Biol. 2011 Dec;41(12):938-45. (PMID: 21968404)
J Biol Chem. 2014 Oct 3;289(40):27874-85. (PMID: 25122763)
Proc Natl Acad Sci U S A. 2021 Sep 14;118(37):. (PMID: 34493670)
Proc Natl Acad Sci U S A. 2021 Sep 28;118(39):. (PMID: 34544864)
Sci Rep. 2020 Sep 1;10(1):14394. (PMID: 32873850)
PLoS One. 2012;7(8):e43784. (PMID: 22928033)
Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4024-9. (PMID: 15024118)
معلومات مُعتمدة: KQTD20180411143628272 the Shenzhen Science and Technology Program; 31930014 the National Natural Science Foundation of China; 32220103003 the National Natural Science Foundation of China; 32200398 the National Natural Science Foundation of China; 32100375 the National Natural Science Foundation of China; NT2021003 the Laboratory of Lingnan Modern Agriculture Project; 2019A0102006 the Department of Science and Technology in Guangdong Province; 2022A1515010938 the Natural Science Foundation in Guangdong Province; 2023A1515010409 the Natural Science Foundation in Guangdong Province; SL2023A04J01127 the "Xu Hang" Project for Excellent Doctors of Guangzhou City Science and Technology Plan Projects
فهرسة مساهمة: Keywords: CYP15; Cyp6g2; Epoxidase; Farnesoic acid (FA); Juvenile hormone (JH)
المشرفين على المادة: 9035-51-2 (Cytochrome P-450 Enzyme System)
0 (Drosophila Proteins)
EC 1.- (epoxidase)
0 (Juvenile Hormones)
EC 1.- (Oxidoreductases)
EC 1.- (cytochrome P-450 6G2, Drosophila)
تواريخ الأحداث: Date Created: 20240513 Date Completed: 20240514 Latest Revision: 20240712
رمز التحديث: 20240712
مُعرف محوري في PubMed: PMC11092216
DOI: 10.1186/s12915-024-01910-4
PMID: 38741075
قاعدة البيانات: MEDLINE
الوصف
تدمد:1741-7007
DOI:10.1186/s12915-024-01910-4