دورية أكاديمية
Identifying Individual Pseudouridine (Ψ) Sites Across Transcripts from HIV-1 Infected Cells.
| العنوان: | Identifying Individual Pseudouridine (Ψ) Sites Across Transcripts from HIV-1 Infected Cells. |
|---|---|
| المؤلفون: | Coutts HL; Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast, UK., Courtney DG; Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast, UK. david.courtney@qub.ac.uk. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2807, pp. 229-242. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Pseudouridine*/metabolism , Pseudouridine*/genetics , HIV-1*/genetics , RNA, Viral*/genetics , RNA, Messenger*/genetics , RNA, Messenger*/metabolism, Humans ; HIV Infections/virology ; HIV Infections/genetics ; RNA Processing, Post-Transcriptional ; Cell Line |
| مستخلص: | The identification of RNA modifications at single nucleotide resolution has become an emerging area of interest within biology and specifically among virologists seeking to ascertain how this untapped area of RNA regulation may be altered or hijacked upon viral infection. Herein, we describe a straightforward biochemical approach modified from two original published Ψ mapping protocols, BID-seq and PRAISE, to specifically identify pseudouridine modifications on mRNA transcripts from an HIV-1 infected T cell line. This protocol could readily be adapted for other viral infected cell types and additionally for populations of purified virions from infected cells. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Bisulfite; Epitranscriptomics; HIV-1; Mapping; Modifications; Pseudouridine; RNA; Virus |
| تواريخ الأحداث: | Date Created: 20240514 Date Completed: 20240514 Latest Revision: 20240514 |
| رمز التحديث: | 20240515 |
| DOI: | 10.1007/978-1-0716-3862-0_16 |
| PMID: | 38743232 |
| قاعدة البيانات: | MEDLINE |
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