دورية أكاديمية
أعرض في EDS

The role of the Golden2-like (GLK) transcription factor in regulating terpenoid indole alkaloid biosynthesis in Catharanthus roseus.

التفاصيل البيبلوغرافية
العنوان: The role of the Golden2-like (GLK) transcription factor in regulating terpenoid indole alkaloid biosynthesis in Catharanthus roseus.
المؤلفون: Cole-Osborn LF; Department of Chemical Engineering, Northeastern University, Boston, MA, 02115, USA.; Department of Bioengineering, Northeastern University, Boston, USA., McCallan SA; Department of Chemistry and Chemical Biology, Northeastern University, Boston, USA., Prifti O; Department of Bioengineering, Northeastern University, Boston, USA., Abu R; Department of Chemistry and Chemical Biology, Northeastern University, Boston, USA., Sjoelund V; Department of Chemistry and Chemical Biology, Northeastern University, Boston, USA., Lee-Parsons CWT; Department of Chemical Engineering, Northeastern University, Boston, MA, 02115, USA. ca.lee@northeastern.edu.; Department of Bioengineering, Northeastern University, Boston, USA. ca.lee@northeastern.edu.; Department of Chemistry and Chemical Biology, Northeastern University, Boston, USA. ca.lee@northeastern.edu.
المصدر: Plant cell reports [Plant Cell Rep] 2024 May 14; Vol. 43 (6), pp. 141. Date of Electronic Publication: 2024 May 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin ; New York : Springer, 1981-
مواضيع طبية MeSH: Catharanthus*/genetics , Catharanthus*/metabolism , Gene Expression Regulation, Plant* , Secologanin Tryptamine Alkaloids*/metabolism , Plant Proteins*/metabolism , Plant Proteins*/genetics , Transcription Factors*/metabolism , Transcription Factors*/genetics , Gene Silencing*, Plant Leaves/metabolism ; Plant Leaves/genetics ; Chloroplasts/metabolism
مستخلص: Key Message: A GLK homologue was identified and functionally characterized in Catharanthus roseus. Silencing CrGLK with VIGS or the chloroplast retrograde signaling inducer lincomycin increased terpenoid indole alkaloid biosynthesis. Catharanthus roseus is the sole source of the chemotherapeutic terpenoid indole alkaloids (TIAs) vinblastine and vincristine. TIA pathway genes, particularly genes in the vindoline pathway, are expressed at higher levels in immature versus mature leaves, but the molecular mechanisms responsible for this developmental regulation are unknown. We investigated the role of GOLDEN2-LIKE (GLK) transcription factors in contributing to this ontogenetic regulation since GLKs are active in seedlings upon light exposure and in the leaf's early development, but their activity is repressed as leaves age and senesce. We identified a GLK homologue in C. roseus and functionally characterized its role in regulating TIA biosynthesis, with a focus on the vindoline pathway, by transiently reducing its expression through two separate methods: virus-induced gene silencing (VIGS) and application of chloroplast retrograde signaling inducers, norflurazon and lincomycin. Reducing CrGLK levels with each method reduced chlorophyll accumulation and the expression of the light harvesting complex subunit (LHCB2.2), confirming its functional homology with GLKs in other plant species. In contrast, reducing CrGLK via VIGS or lincomycin increased TIA accumulation and TIA pathway gene expression, suggesting that CrGLK may repress TIA biosynthesis. However, norflurazon had no effect on TIA gene expression, indicating that reducing CrGLK alone is not sufficient to induce TIA biosynthesis. Future work is needed to clarify the specific molecular mechanisms leading to increased TIA biosynthesis with CrGLK silencing. This is the first identification and characterization of GLK in C. roseus and the first investigation of how chloroplast retrograde signaling might regulate TIA biosynthesis.
(© 2024. The Author(s).)
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معلومات مُعتمدة: Acorn Grant Massachusetts Technology Transfer Center; GapFund360 Award (GAP1-2005) Northeastern University
فهرسة مساهمة: Keywords: Catharanthus roseus; Chloroplast retrograde signaling; GLK; Golden2-like transcription factor; Lincomycin; Terpenoid indole alkaloid
تواريخ الأحداث: Date Created: 20240514 Date Completed: 20240514 Latest Revision: 20240619
رمز التحديث: 20240619
مُعرف محوري في PubMed: PMC11093837
DOI: 10.1007/s00299-024-03208-9
PMID: 38743349
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-203X
DOI:10.1007/s00299-024-03208-9