Alterations of PINK1-PRKN signaling in mice during normal aging.
| العنوان: | Alterations of PINK1-PRKN signaling in mice during normal aging. |
|---|---|
| المؤلفون: | Baninameh Z, Watzlawik JO, Hou X, Richardson T, Kurchaba NW, Yan T, Di Florio DN, Fairweather D, Kang L, Nguyen JH, Kanekiyo T, Dickson DW, Noda S, Sato S, Hattori N, Goldberg MS, Ganley IG, Stauch KL, Fiesel FC, Springer W |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2024 May 01. Date of Electronic Publication: 2024 May 01. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | The ubiquitin kinase-ligase pair PINK1-PRKN identifies and selectively marks damaged mitochondria for elimination via the autophagy-lysosome system (mitophagy). While this cytoprotective pathway has been extensively studied in vitro upon acute and complete depolarization of mitochondria, the significance of PINK1-PRKN mitophagy in vivo is less well established. Here we used a novel approach to study PINK1-PRKN signaling in different energetically demanding tissues of mice during normal aging. We demonstrate a generally increased expression of both genes and enhanced enzymatic activity with aging across tissue types. Collectively our data suggest a distinct regulation of PINK1-PRKN signaling under basal conditions with the most pronounced activation and flux of the pathway in mouse heart compared to brain or skeletal muscle. Our biochemical analyses complement existing mitophagy reporter readouts and provide an important baseline assessment in vivo, setting the stage for further investigations of the PINK1-PRKN pathway during stress and in relevant disease conditions. |
| معلومات مُعتمدة: | R01 HL164520 United States HL NHLBI NIH HHS; R21 AI145356 United States AI NIAID NIH HHS; R21 AI154927 United States AI NIAID NIH HHS |
| تواريخ الأحداث: | Date Created: 20240515 Latest Revision: 20240521 |
| رمز التحديث: | 20240521 |
| مُعرف محوري في PubMed: | PMC11092476 |
| DOI: | 10.1101/2024.04.29.591753 |
| PMID: | 38746191 |
| قاعدة البيانات: | MEDLINE |
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