دورية أكاديمية
أعرض في EDS

egr3 is a mechanosensitive transcription factor gene required for cardiac valve morphogenesis.

التفاصيل البيبلوغرافية
العنوان: egr3 is a mechanosensitive transcription factor gene required for cardiac valve morphogenesis.
المؤلفون: da Silva AR; Max Planck Institute for Heart and Lung Research, Department of Developmental Genetics, Bad Nauheim, Germany.; German Centre for Cardiovascular Research (DZHK), Partner Site Rhine-Main, Bad Nauheim, Germany.; Cardio-Pulmonary Institute (CPI), Bad Nauheim, Germany., Gunawan F; Max Planck Institute for Heart and Lung Research, Department of Developmental Genetics, Bad Nauheim, Germany.; German Centre for Cardiovascular Research (DZHK), Partner Site Rhine-Main, Bad Nauheim, Germany.; Cardio-Pulmonary Institute (CPI), Bad Nauheim, Germany., Boezio GLM; Max Planck Institute for Heart and Lung Research, Department of Developmental Genetics, Bad Nauheim, Germany.; German Centre for Cardiovascular Research (DZHK), Partner Site Rhine-Main, Bad Nauheim, Germany.; Cardio-Pulmonary Institute (CPI), Bad Nauheim, Germany., Faure E; Aix Marseille Université, INSERM, MMG, U1251, 13005 Marseille, France., Théron A; Aix Marseille Université, INSERM, MMG, U1251, 13005 Marseille, France.; Service de Chirurgie Cardiaque, AP-HM, Hôpital de la Timone, 13005 Marseille, France., Avierinos JF; Aix Marseille Université, INSERM, MMG, U1251, 13005 Marseille, France.; Service de Cardiologie, AP-HM, Hôpital de la Timone, 13005 Marseille, France., Lim S; Max Planck Institute for Heart and Lung Research, Department of Developmental Genetics, Bad Nauheim, Germany., Jha SG; Max Planck Institute for Heart and Lung Research, Department of Developmental Genetics, Bad Nauheim, Germany., Ramadass R; Max Planck Institute for Heart and Lung Research, Department of Developmental Genetics, Bad Nauheim, Germany., Guenther S; German Centre for Cardiovascular Research (DZHK), Partner Site Rhine-Main, Bad Nauheim, Germany.; Cardio-Pulmonary Institute (CPI), Bad Nauheim, Germany.; Bioinformatics and Deep Sequencing Platform, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany., Looso M; German Centre for Cardiovascular Research (DZHK), Partner Site Rhine-Main, Bad Nauheim, Germany.; Cardio-Pulmonary Institute (CPI), Bad Nauheim, Germany.; Bioinformatics Core Unit (BCU), Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany., Zaffran S; Aix Marseille Université, INSERM, MMG, U1251, 13005 Marseille, France., Juan T; Max Planck Institute for Heart and Lung Research, Department of Developmental Genetics, Bad Nauheim, Germany.; German Centre for Cardiovascular Research (DZHK), Partner Site Rhine-Main, Bad Nauheim, Germany.; Cardio-Pulmonary Institute (CPI), Bad Nauheim, Germany., Stainier DYR; Max Planck Institute for Heart and Lung Research, Department of Developmental Genetics, Bad Nauheim, Germany.; German Centre for Cardiovascular Research (DZHK), Partner Site Rhine-Main, Bad Nauheim, Germany.; Cardio-Pulmonary Institute (CPI), Bad Nauheim, Germany.
المصدر: Science advances [Sci Adv] 2024 May 17; Vol. 10 (20), pp. eadl0633. Date of Electronic Publication: 2024 May 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : American Association for the Advancement of Science, [2015]-
مواضيع طبية MeSH: Zebrafish* , Heart Valves*/metabolism , Heart Valves*/embryology , Zebrafish Proteins*/genetics , Zebrafish Proteins*/metabolism , Morphogenesis*/genetics , Early Growth Response Protein 3*/metabolism , Early Growth Response Protein 3*/genetics, Animals ; Humans ; Gene Expression Regulation, Developmental ; Endothelial Cells/metabolism ; Mechanotransduction, Cellular ; Swine
مستخلص: Biomechanical forces, and their molecular transducers, including key mechanosensitive transcription factor genes, such as KLF2 , are required for cardiac valve morphogenesis. However, klf2 mutants fail to completely recapitulate the valveless phenotype observed under no-flow conditions. Here, we identify the transcription factor EGR3 as a conserved biomechanical force transducer critical for cardiac valve formation. We first show that egr3 null zebrafish display a complete and highly penetrant loss of valve leaflets, leading to severe blood regurgitation. Using tissue-specific loss- and gain-of-function tools, we find that during cardiac valve formation, Egr3 functions cell-autonomously in endothelial cells, and identify one of its effectors, the nuclear receptor Nr4a2b. We further find that mechanical forces up-regulate egr3 / EGR3 expression in the developing zebrafish heart and in porcine valvular endothelial cells, as well as during human aortic valve remodeling. Altogether, these findings reveal that EGR3 is necessary to transduce the biomechanical cues required for zebrafish cardiac valve morphogenesis, and potentially for pathological aortic valve remodeling in humans.
References: Dev Cell. 2017 Nov 6;43(3):274-289.e5. (PMID: 29056552)
PLoS One. 2024 May 8;19(5):e0302926. (PMID: 38718095)
Nat Methods. 2011 Jun;8(6):506-15. (PMID: 21552255)
Development. 2017 Dec 1;144(23):4322-4327. (PMID: 29183943)
Development. 2013 Dec;140(24):4982-7. (PMID: 24257628)
Ann Thorac Surg. 2000 Oct;70(4):1270-4. (PMID: 11081884)
Curr Biol. 2015 May 18;25(10):1354-61. (PMID: 25959969)
J Cell Biol. 2019 Mar 4;218(3):1039-1054. (PMID: 30635353)
Curr Biol. 2011 Nov 22;21(22):1942-8. (PMID: 22079115)
Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2309842121. (PMID: 38194447)
J Mol Cell Cardiol. 2020 Jun;143:51-62. (PMID: 32251670)
Circ Res. 2010 Sep 17;107(6):715-27. (PMID: 20651287)
PLoS One. 2013;8(2):e54891. (PMID: 23457456)
Development. 2011 Oct;138(19):4193-8. (PMID: 21896629)
Nat Rev Cardiol. 2018 Nov;15(11):685-704. (PMID: 30287945)
Elife. 2018 May 15;7:. (PMID: 29762122)
Dev Dyn. 2009 Dec;238(12):3103-10. (PMID: 19890913)
Blood. 2008 Sep 15;112(6):2318-26. (PMID: 18614759)
Nature. 2003 Jan 9;421(6919):172-7. (PMID: 12520305)
Nature. 2016 Jun 29;534(7609):700-4. (PMID: 27357797)
Mol Endocrinol. 2004 Jun;18(6):1546-57. (PMID: 14988426)
Cardiovasc Res. 2014 Dec 1;104(3):443-55. (PMID: 25344368)
J Lipid Atheroscler. 2021 May;10(2):145-159. (PMID: 34095009)
Development. 2016 Jun 15;143(12):2217-27. (PMID: 27302398)
Nature. 2010 Mar 4;464(7285):108-11. (PMID: 20154733)
Eur Heart J. 2017 Sep 21;38(36):2739-2791. (PMID: 28886619)
Nat Commun. 2023 Jan 13;14(1):214. (PMID: 36639367)
Cardiovasc Res. 2014 Oct 1;104(1):49-60. (PMID: 25100766)
Development. 2016 Feb 1;143(3):373-86. (PMID: 26839341)
J Biol Chem. 2019 Oct 18;294(42):15271-15281. (PMID: 31522138)
Development. 2008 Mar;135(6):1179-87. (PMID: 18272595)
Nature. 2005 Sep 8;437(7056):270-4. (PMID: 16025100)
Am J Pathol. 2013 Oct;183(4):1197-1208. (PMID: 23906810)
J Formos Med Assoc. 2007 Mar;106(3):181-6. (PMID: 17389161)
Nat Protoc. 2006;1(2):960-7. (PMID: 17406330)
Development. 2013 Nov;140(21):4426-34. (PMID: 24089470)
PLoS Biol. 2004 May;2(5):E129. (PMID: 15138499)
Nat Genet. 1998 Sep;20(1):87-91. (PMID: 9731539)
Front Pediatr. 2020 Jul 08;8:375. (PMID: 32733829)
Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):13904-9. (PMID: 23918387)
Blood. 2010 Mar 25;115(12):2520-32. (PMID: 19965691)
Elife. 2023 Apr 20;12:. (PMID: 37078699)
Arterioscler Thromb Vasc Biol. 2003 Nov 1;23(11):2002-7. (PMID: 14525795)
Am J Pathol. 1996 Jul;149(1):293-305. (PMID: 8686754)
Genes Dev. 2008 Mar 15;22(6):734-9. (PMID: 18347092)
Elife. 2020 May 15;9:. (PMID: 32412410)
Nat Commun. 2013;4:1978. (PMID: 23748970)
J Heart Valve Dis. 2004 Nov;13(6):894-903. (PMID: 15597579)
Int J Cancer. 2011 Jun 1;128(11):2602-12. (PMID: 20715116)
Nat Genet. 2002 May;31(1):106-10. (PMID: 11967535)
Sci Adv. 2022 Sep 2;8(35):eabn2082. (PMID: 36044573)
PLoS Genet. 2018 Nov 14;14(11):e1007754. (PMID: 30427827)
Development. 2005 Sep;132(18):4193-204. (PMID: 16107477)
Development. 2020 Jul 3;147(13):. (PMID: 32620577)
Oncogene. 2008 May 8;27(21):2989-98. (PMID: 18059339)
Nature. 2022 Jun;606(7914):570-575. (PMID: 35614218)
Nat Commun. 2023 Sep 9;14(1):5567. (PMID: 37689753)
Sci Data. 2017 Aug 29;4:170106. (PMID: 28850122)
Cell Rep. 2020 Jul 14;32(2):107883. (PMID: 32668254)
JACC Heart Fail. 2018 Nov;6(11):951-960. (PMID: 30384913)
J Heart Lung Transplant. 2010 Dec;29(12):1321-9. (PMID: 20674397)
Int J Cardiovasc Imaging. 2022 Jan;38(1):51-59. (PMID: 34374902)
Eur J Cardiothorac Surg. 2014 Aug;46(2):193-7. (PMID: 24335262)
Circulation. 2018 Sep 4;138(10):1025-1038. (PMID: 29567669)
Nat Genet. 2020 Jan;52(1):40-47. (PMID: 31844321)
Nat Protoc. 2008;3(1):59-69. (PMID: 18193022)
Circ Res. 1995 Oct;77(4):665-72. (PMID: 7554111)
Cell Death Dis. 2020 Jan 30;11(1):75. (PMID: 32001672)
Nature. 2020 Dec;588(7836):130-134. (PMID: 33208950)
Elife. 2019 Sep 16;8:. (PMID: 31524599)
Biotechniques. 2006 Mar;40(3):274, 276, 278 passim. (PMID: 16568816)
Circ J. 2014;78(8):1801-7. (PMID: 24998280)
Zebrafish. 2020 Feb;17(1):56-58. (PMID: 31851585)
Nat Commun. 2016 May 25;7:11646. (PMID: 27221222)
PLoS Biol. 2009 Nov;7(11):e1000246. (PMID: 19924233)
Nature. 2022 Aug;608(7921):181-191. (PMID: 35732239)
Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11299-11308. (PMID: 31110021)
Development. 2018 Jul 30;145(14):. (PMID: 30061167)
Development. 2010 Sep;137(18):3119-28. (PMID: 20736288)
Circ Res. 2019 Apr 12;124(8):1163-1165. (PMID: 30973806)
Proc Natl Acad Sci U S A. 2008 Jan 29;105(4):1255-60. (PMID: 18202183)
Lipids Health Dis. 2014 May 21;13:84. (PMID: 24886494)
Curr Top Dev Biol. 2019;132:395-416. (PMID: 30797515)
J Mol Cell Cardiol. 2017 Jul;108:114-126. (PMID: 28576718)
Int J Cardiol. 2021 Jan 15;323:220-228. (PMID: 32858136)
Bio Protoc. 2021 Jul 20;11(14):e4100. (PMID: 34395736)
Elife. 2018 Dec 28;7:. (PMID: 30592462)
Cell Mol Immunol. 2023 Sep;20(9):1051-1062. (PMID: 37264249)
Science. 2021 Oct 15;374(6565):351-354. (PMID: 34648325)
Am J Med Genet. 2001 Aug 15;102(3):266-71. (PMID: 11484205)
Circ Res. 2020 Apr 10;126(8):968-984. (PMID: 32070236)
Nat Rev Cardiol. 2023 Mar;20(3):197-210. (PMID: 36198871)
Circ Res. 2023 Dec 8;133(12):1022-1039. (PMID: 37961886)
Circ Res. 2019 May 24;124(11):1658-1678. (PMID: 31120817)
Transl Psychiatry. 2022 Aug 8;12(1):320. (PMID: 35941129)
Sci Rep. 2022 Mar 21;12(1):4795. (PMID: 35314737)
Development. 2020 Aug 7;147(15):. (PMID: 32769200)
Development. 2018 Jan 3;145(1):. (PMID: 29158447)
Science. 2006 May 5;312(5774):751-3. (PMID: 16675702)
Proc Natl Acad Sci U S A. 2011 May 17;108(20):8390-5. (PMID: 21540330)
Bioinformatics. 2019 Mar 15;35(6):1055-1057. (PMID: 30535135)
Cell Rep. 2021 Oct 5;37(1):109782. (PMID: 34610316)
Semin Cell Dev Biol. 2022 Oct;130:45-55. (PMID: 35367121)
Dev Cell. 2022 Mar 14;57(5):598-609.e5. (PMID: 35245444)
Dis Model Mech. 2023 May 1;16(5):. (PMID: 36172839)
J Am Coll Cardiol. 2017 Jul 11;70(2):252-289. (PMID: 28315732)
تواريخ الأحداث: Date Created: 20240515 Date Completed: 20240515 Latest Revision: 20240801
رمز التحديث: 20240801
مُعرف محوري في PubMed: PMC11095463
DOI: 10.1126/sciadv.adl0633
PMID: 38748804
قاعدة البيانات: MEDLINE
الوصف
تدمد:2375-2548
DOI:10.1126/sciadv.adl0633