دورية أكاديمية
A simplified method of bacteriophage preparation for transmission electron microscope.
| العنوان: | A simplified method of bacteriophage preparation for transmission electron microscope. |
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| المؤلفون: | Meidaninikjeh S; Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran., Mohammadi P; Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran; Research Center for Applied Microbiology and Microbial Biotechnology, Alzahra University, Tehran, Iran. Electronic address: p.mohammadi@alzahra.ac.ir., Elikaei A; Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran; Research Center for Applied Microbiology and Microbial Biotechnology, Alzahra University, Tehran, Iran. |
| المصدر: | Journal of virological methods [J Virol Methods] 2024 Jul; Vol. 328, pp. 114951. Date of Electronic Publication: 2024 May 14. |
| نوع المنشور: | Journal Article; Comparative Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980- |
| مواضيع طبية MeSH: | Microscopy, Electron, Transmission* , Bacteriophages*/ultrastructure , Bacteriophages*/isolation & purification, Viral Plaque Assay ; Specimen Handling/methods ; Microbial Viability ; Virology/methods ; Water |
| مستخلص: | Bacteriophages are viruses that infect bacteria. Researchers use different methods to study the characteristics of bacteriophages. Transmission electron microscope (TEM) is considered the best method to analyze these characteristics. However, the quality of TEM micrographs is significantly influenced by the preparation methods used to prepare the bacteriophages sample. In this study, researchers compared two different methods for preparing the bacteriophage samples. In one method was used SM buffer, while in the other used deionized water. The results were analyzed by TEM and compared with each other. Additionally, the viability of bacteriophage in deionized water and SM buffer at 4°C was determined through plaque assay within 72 hours. TEM micrographs showed that the quality of bacteriophage sample prepared with deionized water is superior to those prepared with SM buffer. Furthermore, the titer of the bacteriophages did not show a significant reduction during 72 hours in both SM and deionized water. In conclusion, the results suggested that preparation method can significantly impact the quality of TEM micrographs. Using sterile deionized water for the preparation of bacteriophages is a simple way to improve the quality of TEM micrographs and it is advisable to send the samples to the laboratory within 72 hours. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Deionized water; Electro-microscopic preparation technique; SM buffer; Storage; TEM; Viability |
| المشرفين على المادة: | 059QF0KO0R (Water) |
| تواريخ الأحداث: | Date Created: 20240516 Date Completed: 20240614 Latest Revision: 20240614 |
| رمز التحديث: | 20240615 |
| DOI: | 10.1016/j.jviromet.2024.114951 |
| PMID: | 38750823 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0984 |
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| DOI: | 10.1016/j.jviromet.2024.114951 |