دورية أكاديمية
أعرض في EDS

Successful Extension of Vascularized Composite Allograft Perfusion Cold Storage to 24 h in a Rat Hindlimb Transplant Model.

التفاصيل البيبلوغرافية
العنوان: Successful Extension of Vascularized Composite Allograft Perfusion Cold Storage to 24 h in a Rat Hindlimb Transplant Model.
المؤلفون: Ng PK; Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO., Yoeli D; Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO., Huang JL; Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO., Luo Y; Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO., Wang Y; Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO., Li B; Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO., Wang Z; Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO., Schold J; Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO., Jain S; Department of Medicine, University of Colorado Anschutz Medical Campus , Aurora, CO., Su AA; Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO., Mathes DW; Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO., Washington KM; Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO., Farkash E; Department of Pathology, University of Michigan School of Medicine, Ann Arbor, MI., Jani AH; Department of Medicine, University of Colorado Anschutz Medical Campus , Aurora, CO., Huang CA; Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO.
المصدر: Transplantation direct [Transplant Direct] 2024 May 15; Vol. 10 (6), pp. e1623. Date of Electronic Publication: 2024 May 15 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wolters Kluwer Country of Publication: United States NLM ID: 101651609 Publication Model: eCollection Cited Medium: Print ISSN: 2373-8731 (Print) Linking ISSN: 23738731 NLM ISO Abbreviation: Transplant Direct Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Philadelphia, Pa.] : Wolters Kluwer, [2015]-
مستخلص: Background: Vascularized composite allograft transplantation is a treatment option for complex tissue injuries; however, ischemia reperfusion injury and high acute rejection rates remain a challenge. Hypothermic machine perfusion using acellular storage perfusate is a potential solution. This study evaluated the University of Wisconsin Kidney Preservation Solution-1 (KPS-1) compared with normal saline (NS) for preservation of donor rat hindlimbs subjected to 24 h of ex vivo perfusion cold storage.
Methods: Hindlimbs were subjected to 24-h perfusion cold storage with heparinized KPS-1 (n = 6) or heparinized NS (n = 6). Flow, resistance, and pH were measured continuously. At the end of the 24-h period, tissue was collected for histological analysis of edema and apoptosis.
Results: KPS-1 perfused limbs showed significantly less edema than the NS group, as evidenced by lower limb weight gain ( P  < 0.001) and less interfascicular space ( P  < 0.001). KPS-perfused muscle had significantly less cell death than NS-perfused muscle based on terminal deoxynucleotidyl transferase dUTP nick-end labeling ( P  < 0.001) and cleaved caspase-3 staining ( P  = 0.045). During hypothermic machine perfusion, a significant decrease in pH over time was detected in both groups, with a significantly greater decline in pH in the KPS-1 group than in the NS group. There were no significant differences overall and over time in flow rate or vascular resistance between the KPS and NS groups.
Conclusions: Perfusion with KPS-1 can successfully extend vascularized composite allograft perfusion cold storage for 24 h in a rat hindlimb model without significant edema or cell death.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2024 The Author(s). Transplantation Direct. Published by Wolters Kluwer Health, Inc.)
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معلومات مُعتمدة: T32 TR004366 United States TR NCATS NIH HHS; UM1 TR004399 United States TR NCATS NIH HHS
تواريخ الأحداث: Date Created: 20240517 Latest Revision: 20240604
رمز التحديث: 20240604
مُعرف محوري في PubMed: PMC11098188
DOI: 10.1097/TXD.0000000000001623
PMID: 38757052
قاعدة البيانات: MEDLINE
الوصف
تدمد:2373-8731
DOI:10.1097/TXD.0000000000001623