Bioinformatics proved the existence of potential hub genes activating autophagy to participate in cartilage degeneration in osteonecrosis of the femoral head.

التفاصيل البيبلوغرافية
العنوان:	Bioinformatics proved the existence of potential hub genes activating autophagy to participate in cartilage degeneration in osteonecrosis of the femoral head.
المؤلفون:	Zhu Y; Department of Orthopedics, The Second Affiliated Hospital of Xi' an Jiaotong University, Xi'an, 710004, China., Wang X; Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada., Liu R; Department of Orthopedics, The Second Affiliated Hospital of Xi' an Jiaotong University, Xi'an, 710004, China. liuryu@126.com.
المصدر:	Journal of molecular histology [J Mol Histol] 2024 Aug; Vol. 55 (4), pp. 539-554. Date of Electronic Publication: 2024 May 17.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Springer Netherlands Country of Publication: Netherlands NLM ID: 101193653 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1567-2387 (Electronic) Linking ISSN: 15672379 NLM ISO Abbreviation: J Mol Histol Subsets: MEDLINE
أسماء مطبوعة:	Publication: Dordrecht, The Netherlands : Springer Netherlands Original Publication: Dordrecht, The Netherlands : Kluwer Academic Publishers, 2004-
مواضيع طبية MeSH:	Autophagy/genetics , Computational Biology/methods , Protein Interaction Maps/genetics , Gene Regulatory Networks , Femur Head Necrosis/genetics , Femur Head Necrosis/pathology , Femur Head Necrosis*/metabolism, Humans ; Gene Expression Profiling ; Chondrocytes/metabolism ; Chondrocytes/pathology ; Cartilage, Articular/metabolism ; Cartilage, Articular/pathology ; Femur Head/pathology ; Femur Head/metabolism ; Gene Expression Regulation ; Databases, Genetic ; Autophagy-Related Proteins/genetics ; Autophagy-Related Proteins/metabolism
مستخلص:	The obvious degeneration of articular cartilage occurs in the late stage of osteonecrosis of the femoral head (ONFH), which aggravates the condition of ONFH. This study aimed to demonstrate aberrant activation of autophagy processes in ONFH chondrocytes through bioinformatics and to predict and identify relevant hub genes and pathways. Differentially expressed genes (DEGs) were identified using R software in the GSE74089 dataset from the GEO database. DEGs were crossed with the Human Autophagy Database (HADb) autophagy genes to screen out autophagy-related differential genes (AT-DEGs). GSEA, GSVA, GO, and KEGG pathway enrichment analyses of AT-DEGs were performed. The STRING database was used to analyze the protein-protein interaction (PPI) of the AT-DEGs network, and the MCODE and CytoHubba plugin in the Cytoscape software was used to analyze the key gene cluster module and screen the hub genes. The PPI network of hub genes was constructed using the GeneMANIA database, and functional enrichment and gene connectivity categories were analyzed. The expression levels of hub genes of related genes in the ONFH patients were verified in the dataset GSE123568, and the protein expression was verified by immunohistochemistry in tissues. The analysis of DEGs revealed abnormal autophagy in ONFH cartilage. AT-DEGs in ONFH have special enrichment in macroautophagy, autophagosome membrane, and phosphatidylinositol-3-phosphate binding. In the GSE123568 dataset, it was also found that ATG2B, ATG4B, and UVRAG were all significantly upregulated in ONFH patients. By immunohistochemistry, it was verified that ATG2B, ATG4B, and UVRAG were significantly overexpressed. These three genes regulate the occurrence and extension of autophagosomes through the PI3KC3C pathway. Finally, we determined that chondrocytes in ONFH undergo positive regulation of autophagy through the corresponding pathways involved in three genes: ATG2B, ATG4B, and UVRAG. (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
References:	Baeken MW, Weckmann K, Diefenthaler P, Schulte J, Yusifli K, Moosmann B, Behl C et al (2020) Novel insights into the Cellular localization and regulation of the Autophagosomal proteins LC3A, LC3B and LC3C. Cells 9(10). https://doi.org/10.3390/cells9102315. Bohensky J, Shapiro IM, Leshinsky S, Terkhorn SP, Adams CS, Srinivas V (2007) HIF-1 regulation of chondrocyte apoptosis: induction of the autophagic pathway. Autophagy 3(3):207–214. https://doi.org/10.4161/auto.3708. (PMID: 10.4161/auto.370817224629) Caron MM, Emans PJ, Surtel DA, Cremers A, Voncken JW, Welting TJ, van Rhijn LW (2012) Activation of NF-kappaB/p65 facilitates early chondrogenic differentiation during endochondral ossification. PLoS ONE 7(3):e33467. https://doi.org/10.1371/journal.pone.0033467. (PMID: 10.1371/journal.pone.0033467224280553299787) Chen X, Xue W, Zhang J, Peng J, Huang W (2023) Ginsenoside Rg1 attenuates the NASH phenotype by regulating the miR-375-3p/ATG2B/PTEN-AKT axis to mediate autophagy and pyroptosis. Lipids Health Dis 22(1):22. https://doi.org/10.1186/s12944-023-01787-2. (PMID: 10.1186/s12944-023-01787-2367598379912620) Debnath J, Gammoh N, Ryan KM (2023) Autophagy and autophagy-related pathways in cancer. Nat Rev Mol Cell Biol 24(8):560–575. https://doi.org/10.1038/s41580-023-00585-z. (PMID: 10.1038/s41580-023-00585-z36864290) Duan R, Xie H, Liu ZZ (2020) The role of Autophagy in Osteoarthritis. Front Cell Dev Biol 8:608388. https://doi.org/10.3389/fcell.2020.608388. (PMID: 10.3389/fcell.2020.608388333246547723985) Fernandez AF, Sebti S, Wei Y, Zou Z, Shi M, McMillan KL, He C et al (2018) Disruption of the beclin 1-BCL2 autophagy regulatory complex promotes longevity in mice. Nature 558(7708):136–140. https://doi.org/10.1038/s41586-018-0162-7. (PMID: 10.1038/s41586-018-0162-7298491495992097) Franz M, Rodriguez H, Lopes C, Zuberi K, Montojo J, Bader GD, Morris Q (2018) GeneMANIA update 2018. Nucleic Acids Res 46(W1). https://doi.org/10.1093/nar/gky311 . W60-W64. Gao S, Wang K, Wang X (2020) miR-375 targeting autophagy-related 2B (ATG2B) suppresses autophagy and tumorigenesis in cisplatin-resistant osteosarcoma cells. Neoplasma 67(4):724–734. https://doi.org/10.4149/neo_2020_190423N366. (PMID: 10.4149/neo_2020_190423N36632726125) Gao YH, Dong N, Yang C, Li SQ, Liu JG, Qi X (2020) Elevated synovial fluid IL-33 and IL-6 levels and cartilage degeneration in stage III osteonecrosis of the femoral head. Technol Health Care 28(2):203–212. https://doi.org/10.3233/THC-191811. (PMID: 10.3233/THC-19181131594271) Guo P, Li H, Wang X, Li X, Li X (2023) PG545 Prevents Osteoarthritis Development by Regulating PI3K/AKT/mTOR Signaling and Activating Chondrocyte Autophagy. Pharmacology: 1. https://doi.org/10.1159/000532078. Han Y, Si M, Zhao Y, Liu Y, Cheng K, Zhang Y, Jia J et al (2017) Progranulin protects against osteonecrosis of the femoral head by activating ERK1/2 pathway. Inflammation 40(3):946–955. https://doi.org/10.1007/s10753-017-0539-z. (PMID: 10.1007/s10753-017-0539-z28247166) Han Y, Zhang L, Xing Y, Zhang L, Chen X, Tang P, Chen Z (2018) Autophagy relieves the function inhibition and apoptosis–promoting effects on osteoblast induced by glucocorticoid. Int J Mol Med 41(2):800–808. https://doi.org/10.3892/ijmm.2017.3270. (PMID: 10.3892/ijmm.2017.327029207032) Hu YX, Han XS, Jing Q (2019) Autophagy in development and differentiation. Adv Exp Med Biol 1206:469–487. https://doi.org/10.1007/978-981-15-0602-4_22. (PMID: 10.1007/978-981-15-0602-4_2231776999) Hua F, Li K, Shang S, Wang F, Hu Z (2019) Immune Signaling and Autophagy Regulation. Adv Exp Med Biol 1206:551–593. https://doi.org/10.1007/978-981-15-0602-4_26. (PMID: 10.1007/978-981-15-0602-4_2631777003) Imagawa Y, Saitoh T, Tsujimoto Y (2016) Vital staining for cell death identifies Atg9a-dependent necrosis in developmental bone formation in mouse. Nat Commun 7:13391. https://doi.org/10.1038/ncomms13391. (PMID: 10.1038/ncomms13391278118525097171) Itoh S, Saito T, Hirata M, Ushita M, Ikeda T, Woodgett JR, Algul H et al (2012) GSK-3alpha and GSK-3beta proteins are involved in early stages of chondrocyte differentiation with functional redundancy through RelA protein phosphorylation. J Biol Chem 287(35):29227–29236. https://doi.org/10.1074/jbc.M112.372086. (PMID: 10.1074/jbc.M112.372086227614463436165) Jia Y, Zhang Y, Li S, Li R, Li W, Li T, Wang R et al (2023) Identification and assessment of novel dynamic biomarkers for monitoring non-traumatic osteonecrosis of the femoral head staging. Clin Transl Med 13(6):e1295. https://doi.org/10.1002/ctm2.1295. (PMID: 10.1002/ctm2.12953731369210265435) Jingushi S, Lohmander LS, Shinmei M, Hoerrner LA, Lark MW, Sugioka Y, Iwamoto Y (2000) Markers of joint tissue turnover in joint fluids from hips with osteonecrosis of the femoral head. J Orthop Res 18(5):728–733. https://doi.org/10.1002/jor.1100180508. (PMID: 10.1002/jor.110018050811117293) Kobayashi H, Chang SH, Mori D, Itoh S, Hirata M, Hosaka Y, Taniguchi Y et al (2016) Biphasic regulation of chondrocytes by Rela through induction of anti-apoptotic and catabolic target genes. Nat Commun 7:13336. https://doi.org/10.1038/ncomms13336. (PMID: 10.1038/ncomms13336278307065109547) Kong P, Ahmad RE, Zulkifli A, Krishnan S, Nam HY, Kamarul T (2023) The role of autophagy in mitigating osteoarthritis progression via regulation of chondrocyte apoptosis: a review. Joint Bone Spine 91(3):105642. https://doi.org/10.1016/j.jbspin.2023.105642. (PMID: 10.1016/j.jbspin.2023.10564237739213) Li JK, Cheng L, Zhao YP, Guo YJ, Liu Y, Zhang W, Wang SS et al (2015) ADAMTS-7 exhibits elevated expression in cartilage of osteonecrosis of femoral head and has a positive correlation with TNF- alpha and NF- kappa B P65. Mediators Inflamm 2015: 196702. https://doi.org/10.1155/2015/196702. Li Z, Wang L, Wei J, Zhu L, Weng X, Jin J, Xiao H et al (2017) Bone-strengthening pill (BSP) promotes bone cell and chondrocyte repair, and the clinical and experimental study of BSP in the treatment of osteonecrosis of the femoral head. Oncotarget 8(57):97079–97089. https://doi.org/10.18632/oncotarget.21226. (PMID: 10.18632/oncotarget.21226292285945722546) Li L, Tong M, Fu Y, Chen F, Zhang S, Chen H, Ma X et al (2021) Lipids and membrane-associated proteins in autophagy. Protein Cell 12(7):520–544. https://doi.org/10.1007/s13238-020-00793-9. (PMID: 10.1007/s13238-020-00793-933151516) Liang XZ, Luo D, Chen YR, Li JC, Yan BZ, Guo YB, Wen MT et al (2022) Identification of potential autophagy-related genes in steroid-induced osteonecrosis of the femoral head via bioinformatics analysis and experimental verification. J Orthop Surg Res 17(1):86. https://doi.org/10.1186/s13018-022-02977-x. (PMID: 10.1186/s13018-022-02977-x351513598840318) Liu C, Wang B, Xiao L, Li Y, Xu L, Zhao Z, Zhang L (2018) Protective effects of the pericellular matrix of chondrocyte on articular cartilage against the development of osteoarthritis. Histol Histopathol 33(8):757–764. https://doi.org/10.14670/HH-11-967. (PMID: 10.14670/HH-11-96729359311) Liu K, Fan R, Zhou Z (2021) Endoplasmic reticulum stress, chondrocyte apoptosis and oxidative stress in cartilage of broilers affected by spontaneous femoral head necrosis. Poult Sci 100(8):101258. https://doi.org/10.1016/j.psj.2021.101258. (PMID: 10.1016/j.psj.2021.101258341757988242058) Luo P, Gao F, Han J, Sun W, Li Z (2018) The role of autophagy in steroid necrosis of the femoral head: a comprehensive research review. Int Orthop 42(7):1747–1753. https://doi.org/10.1007/s00264-018-3994-8. (PMID: 10.1007/s00264-018-3994-829797168) Ma J, Ge J, Cheng L, Wang B, Yue D, Wang W (2023) Subchondral bone plate classification: a New and more Sensitive Approach for Predicting the prognosis of osteonecrosis of the femoral head. Cartilage 14(3):269–277. https://doi.org/10.1177/19476035231173096. (PMID: 10.1177/194760352311730963721172310601559) Moriya K, Nakano T, Honda Y, Tsumura M, Ogishi M, Sonoda M, Nishitani-Isa M et al (2023) Human RELA dominant-negative mutations underlie type I interferonopathy with autoinflammation and autoimmunity. J Exp Med 220(9). https://doi.org/10.1084/jem.20212276. Mukhopadhyay S, Schlaepfer IR, Bergman BC, Panda PK, Praharaj PP, Naik PP, Agarwal R et al (2017) ATG14 facilitated lipophagy in cancer cells induce ER stress mediated mitoptosis through a ROS dependent pathway. Free Radic Biol Med 104:199–213. https://doi.org/10.1016/j.freeradbiomed.2017.01.007. (PMID: 10.1016/j.freeradbiomed.2017.01.00728069524) Nakatogawa H (2020) Mechanisms governing autophagosome biogenesis. Nat Rev Mol Cell Biol 21(8):439–458. https://doi.org/10.1038/s41580-020-0241-0. (PMID: 10.1038/s41580-020-0241-032372019) Nascimbeni AC, Codogno P, Morel E (2017) Phosphatidylinositol-3-phosphate in the regulation of autophagy membrane dynamics. FEBS J 284(9):1267–1278. https://doi.org/10.1111/febs.13987. (PMID: 10.1111/febs.1398727973739) Osawa T, Ishii Y, Noda NN (2020) Human ATG2B possesses a lipid transfer activity which is accelerated by negatively charged lipids and WIPI4. Genes Cells 25(1):65–70. https://doi.org/10.1111/gtc.12733. (PMID: 10.1111/gtc.1273331721365) Park NY, Jo DS, Cho DH (2022) Post-Translational Modifications of ATG4B in the Regulation of Autophagy. Cells 11(8). https://doi.org/10.3390/cells11081330. Pettenuzzo S, Arduino A, Belluzzi E, Pozzuoli A, Fontanella CG, Ruggieri P, Salomoni V et al (2023) Biomechanics of chondrocytes and chondrons in healthy conditions and osteoarthritis: a review of the mechanical characterisations at the Microscale. Biomedicines 11(7). https://doi.org/10.3390/biomedicines11071942. Prein C, Beier F (2019) ECM signaling in cartilage development and endochondral ossification. Curr Top Dev Biol 133:25–47. https://doi.org/10.1016/bs.ctdb.2018.11.003. (PMID: 10.1016/bs.ctdb.2018.11.00330902255) Qi Y, Wang J, Sun M, Ma C, Jin T, Liu Y, Cao Y et al (2019) MMP-14 single-nucleotide polymorphisms are related to steroid-induced osteonecrosis of the femoral head in the population of northern China. Mol Genet Genomic Med 7(2):e00519. https://doi.org/10.1002/mgg3.519. (PMID: 10.1002/mgg3.51930548828) Qian X, He L, Yang J, Sun J, Peng X, Zhang Y, Mao Y et al (2023) UVRAG cooperates with cargo receptors to assemble the ER-phagy site. EMBO J 42(23):e113625. https://doi.org/10.15252/embj.2023113625. (PMID: 10.15252/embj.202311362537902287) Qin X, Jin P, Jiang T, Li M, Tan J, Wu H, Zheng L et al (2018) A human chondrocyte-derived in Vitro Model of Alcohol-Induced and Steroid-Induced femoral head necrosis. Med Sci Monit 24:539–547. https://doi.org/10.12659/msm.907969. (PMID: 10.12659/msm.907969293744355797332) Qu Y, Liu Y, Li R (2019) FSTL1 promotes inflammatory reaction and cartilage catabolism through interplay with NFkappaB Signaling pathways in an. Vitro ONFH Model Inflamm 42(4):1491–1503. https://doi.org/10.1007/s10753-019-01012-2. (PMID: 10.1007/s10753-019-01012-2) Ren Y, Deng Z, Gokani V, Kutschke M, Mitchell TW, Aruwajoye O, Adapala NS et al (2021) Anti-interleukin-6 therapy decreases hip synovitis and bone resorption and increases bone formation following ischemic osteonecrosis of the femoral head. J Bone Min Res 36(2):357–368. https://doi.org/10.1002/jbmr.4191. (PMID: 10.1002/jbmr.4191) Schmeisser H, Fey SB, Horowitz J, Fischer ER, Balinsky CA, Miyake K, Bekisz J et al (2013) Type I interferons induce autophagy in certain human cancer cell lines. Autophagy 9(5):683–696. https://doi.org/10.4161/auto.23921. (PMID: 10.4161/auto.23921234192693669179) Shen C, Cai GQ, Peng JP, Chen XD (2015) Autophagy protects chondrocytes from glucocorticoids-induced apoptosis via ROS/Akt/FOXO3 signaling. Osteoarthritis Cartilage 23(12):2279–2287. https://doi.org/10.1016/j.joca.2015.06.020. (PMID: 10.1016/j.joca.2015.06.02026165503) Siva Sankar D, Dengjel J (2021) Protein complexes and neighborhoods driving autophagy. Autophagy 17(10):2689–2705. https://doi.org/10.1080/15548627.2020.1847461. (PMID: 10.1080/15548627.2020.184746133183148) Song Z, An L, Ye Y, Wu J, Zou Y, He L, Zhu H (2014) Essential role for UVRAG in autophagy and maintenance of cardiac function. Cardiovasc Res 101(1):48–56. https://doi.org/10.1093/cvr/cvt223. (PMID: 10.1093/cvr/cvt22324081163) Song Y, Quach C, Liang C (2020) UVRAG in autophagy, inflammation, and cancer. Autophagy 16(2):387–388. https://doi.org/10.1080/15548627.2019.1709768. (PMID: 10.1080/15548627.2019.1709768319053126984451) Stanga D, Zhao Q, Milev MP, Saint-Dic D, Jimenez-Mallebrera C, Sacher M (2019) TRAPPC11 functions in autophagy by recruiting ATG2B-WIPI4/WDR45 to preautophagosomal membranes. Traffic 20(5):325–345. https://doi.org/10.1111/tra.12640. (PMID: 10.1111/tra.1264030843302) Sultan AA, Mohamed N, Samuel LT, Chughtai M, Sodhi N, Krebs VE, Stearns KL et al (2019) Classification systems of hip osteonecrosis: an updated review. Int Orthop 43(5):1089–1095. https://doi.org/10.1007/s00264-018-4018-4. (PMID: 10.1007/s00264-018-4018-429916002) Tang Z, Takahashi Y, Chen C, Liu Y, He H, Tsotakos N, Serfass JM et al (2017) Atg2A/B deficiency switches cytoprotective autophagy to non-canonical caspase-8 activation and apoptosis. Cell Death Differ 24(12):2127–2138. https://doi.org/10.1038/cdd.2017.133. (PMID: 10.1038/cdd.2017.133288001315686350) Torzilli PA, Allen SN (2022) Effect of articular Surface Compression on Cartilage Extracellular Matrix deformation. J Biomech Eng 144(9). https://doi.org/10.1115/1.4054108. Tsuchiya M, Ichiseki T, Ueda S, Ueda Y, Shimazaki M, Kaneuji A, Kawahara N (2018) Mitochondrial stress and redox failure in steroid-associated osteonecrosis. Int J Med Sci 15(3):205–209. https://doi.org/10.7150/ijms.22525. (PMID: 10.7150/ijms.22525294838105820848) Velikkakath AK, Nishimura T, Oita E, Ishihara N, Mizushima N (2012) Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Mol Biol Cell 23(5):896–909. https://doi.org/10.1091/mbc.E11-09-0785. (PMID: 10.1091/mbc.E11-09-0785222193743290647) Wang C, Xu H, Liu C, Peng Z, Min R, Zhang Z, Li J et al (2021) CaO(2)/gelatin oxygen slow-releasing microspheres facilitate tissue engineering efficiency for the osteonecrosis of femoral head by enhancing the angiogenesis and survival of grafted bone marrow mesenchymal stem cells. Biomater Sci 9(8):3005–3018. https://doi.org/10.1039/d0bm02071k. (PMID: 10.1039/d0bm02071k33651043) Wang H, Liu X, Yang H, Jing X, Wang W, Liu X, Zhang B et al (2023) Activation of the Nrf-2 pathway by pinocembrin safeguards vertebral endplate chondrocytes against apoptosis and degeneration caused by oxidative stress. Life Sci 333:122162. https://doi.org/10.1016/j.lfs.2023.122162. (PMID: 10.1016/j.lfs.2023.12216237820754) Weidberg H, Shvets E, Shpilka T, Shimron F, Shinder V, Elazar Z (2010) LC3 and GATE-16/GABARAP subfamilies are both essential yet act differently in autophagosome biogenesis. EMBO J 29(11):1792–1802. https://doi.org/10.1038/emboj.2010.74. (PMID: 10.1038/emboj.2010.74204188062885923) Wild P, McEwan DG, Dikic I (2014) The LC3 interactome at a glance. J Cell Sci 127(Pt 1):3–9. https://doi.org/10.1242/jcs.140426. (PMID: 10.1242/jcs.14042624345374) Xiong H, Sun L, Lian J, He F (2023) Involvement of acetylation of ATG4B in controlling autophagy induction. Autophagy 19(3):1039–1041. https://doi.org/10.1080/15548627.2022.2117887. (PMID: 10.1080/15548627.2022.211788736056541) Xu R, Wei B, Li J, Huang C, Lin R, Tang C, Xu Y et al (2017) Investigations of cartilage matrix degeneration in patients with early-stage femoral head necrosis. Med Sci Monit 23:5783–5792. https://doi.org/10.12659/msm.907522. (PMID: 10.12659/msm.907522292088535727749) Xue E, Zhang Y, Song B, Xiao J, Shi Z (2016) Effect of autophagy induced by dexamethasone on senescence in chondrocytes. Mol Med Rep 14(4):3037–3044. https://doi.org/10.3892/mmr.2016.5662. (PMID: 10.3892/mmr.2016.5662275726745042789) Yan X, Zhou R, Ma Z (2019) Autophagy-cell survival and death. Adv Exp Med Biol 1206:667–696. https://doi.org/10.1007/978-981-15-0602-4_29. (PMID: 10.1007/978-981-15-0602-4_2931777006) Yang G, Li Y, Zhao Y, Ouyang L, Chen Y, Liu B, Liu J (2021) Targeting Atg4B for cancer therapy: chemical mediators. Eur J Med Chem 209:112917. https://doi.org/10.1016/j.ejmech.2020.112917. (PMID: 10.1016/j.ejmech.2020.11291733077263) Young MM, Takahashi Y, Khan O, Park S, Hori T, Yun J, Sharma AK et al (2012) Autophagosomal membrane serves as platform for intracellular death-inducing signaling complex (iDISC)-mediated caspase-8 activation and apoptosis. J Biol Chem 287(15):12455–12468. https://doi.org/10.1074/jbc.M111.309104. (PMID: 10.1074/jbc.M111.309104223627823320995) Yu Y, Wang S, Zhou Z (2020) Cartilage homeostasis affects femoral Head Necrosis Induced by Methylprednisolone in broilers. Int J Mol Sci 21(14). https://doi.org/10.3390/ijms21144841. Yu Y, Lin L, Liu K, Jiang Y, Zhou Z (2022) Effects of Simvastatin on Cartilage Homeostasis in Steroid-Induced osteonecrosis of femoral head by inhibiting glucocorticoid receptor. Cells 11(24). https://doi.org/10.3390/cells11243945. Zaman F, Chrysis D, Huntjens K, Chagin A, Takigawa M, Fadeel B, Savendahl L (2014) Dexamethasone differentially regulates Bcl-2 family proteins in human proliferative chondrocytes: role of pro-apoptotic bid. Toxicol Lett 224(2):196–200. https://doi.org/10.1016/j.toxlet.2013.10.020. (PMID: 10.1016/j.toxlet.2013.10.02024172751) Zhang M, Meng N, Wang X, Chen W, Zhang Q (2022) TRPV4 and PIEZO channels mediate the mechanosensing of chondrocytes to the Biomechanical Microenvironment. Membr (Basel) 12(2). https://doi.org/10.3390/membranes12020237. Zhao J, Mu L, Wang Z, Fang X, He X, Zhang X, Xu X (2020) The potential roles of circular RNAs in osteonecrosis of the femoral head (review). Mol Med Rep 21(2):533–539. https://doi.org/10.3892/mmr.2019.10866. (PMID: 10.3892/mmr.2019.1086631974613) Zhou B, Liu J, Kang R, Klionsky DJ, Kroemer G, Tang D (2020) Ferroptosis is a type of autophagy-dependent cell death. Semin Cancer Biol 66:89–100. https://doi.org/10.1016/j.semcancer.2019.03.002. (PMID: 10.1016/j.semcancer.2019.03.00230880243) Zhu R, Wang Y, Ouyang Z, Hao W, Zhou F, Lin Y, Cheng Y et al (2023) Targeting regulated chondrocyte death in osteoarthritis therapy. Biochem Pharmacol 215:115707. https://doi.org/10.1016/j.bcp.2023.115707. (PMID: 10.1016/j.bcp.2023.11570737506921)
فهرسة مساهمة:	Keywords: Autophagy; Autophagy-related genes; Bioinformatics; Chondrocytes; Osteonecrosis of femoral head
المشرفين على المادة:	0 (Autophagy-Related Proteins)
تواريخ الأحداث:	Date Created: 20240517 Date Completed: 20240807 Latest Revision: 20240903
رمز التحديث:	20240903
DOI:	10.1007/s10735-024-10200-w
PMID:	38758521
قاعدة البيانات:	MEDLINE

Find this article in full text from Springer Verlag.

Full Text Finder

الوصف
تدمد:	1567-2387
DOI:	10.1007/s10735-024-10200-w