دورية أكاديمية
أعرض في EDS

OphthalMimic: A new alternative apparatus without animal tissue for the evaluation of topical ophthalmic drug products.

التفاصيل البيبلوغرافية
العنوان: OphthalMimic: A new alternative apparatus without animal tissue for the evaluation of topical ophthalmic drug products.
المؤلفون: Barbalho GN; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900, Brasília, DF, Brazil., Falcão MA; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900, Brasília, DF, Brazil., Alves Amaral V; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900, Brasília, DF, Brazil., Contarato JLA; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900, Brasília, DF, Brazil., Barbalho AM; Electrical, Mechanical and Computer Engineering School, Federal University of Goiás (UFG), 74605-170, Goiânia, GO, Brazil., Kaori Diógenes G; Electrical, Mechanical and Computer Engineering School, Federal University of Goiás (UFG), 74605-170, Goiânia, GO, Brazil., Mariana Gomes Silva M; Electrical, Mechanical and Computer Engineering School, Federal University of Goiás (UFG), 74605-170, Goiânia, GO, Brazil., Carvalho de Barros do Vale Rochelle B; Electrical, Mechanical and Computer Engineering School, Federal University of Goiás (UFG), 74605-170, Goiânia, GO, Brazil., Gelfuso GM; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900, Brasília, DF, Brazil., Cunha-Filho M; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900, Brasília, DF, Brazil., Gratieri T; Laboratory of Food, Drugs, and Cosmetics (LTMAC), University of Brasilia, 70910-900, Brasília, DF, Brazil. Electronic address: tgratieri@unb.br.
المصدر: Methods (San Diego, Calif.) [Methods] 2024 Aug; Vol. 228, pp. 1-11. Date of Electronic Publication: 2024 May 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 9426302 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9130 (Electronic) Linking ISSN: 10462023 NLM ISO Abbreviation: Methods Subsets: MEDLINE
أسماء مطبوعة: Publication: Duluth, MN : Academic Press
Original Publication: San Diego : Academic Press, c1990-
مواضيع طبية MeSH: Poloxamer*/chemistry , Ophthalmic Solutions*/chemistry, Administration, Ophthalmic ; Fluconazole/administration & dosage ; Printing, Three-Dimensional ; Cornea/drug effects ; Cornea/metabolism ; Animals ; Chitosan/chemistry ; Animal Testing Alternatives/methods ; Tears/chemistry ; Humans ; Gelatin/chemistry
مستخلص: The necessity of animal-free performance tests for novel ophthalmic formulation screening is challenging. For this, we developed and validated a new device to simulate the dynamics and physical-chemical barriers of the eye for in vitro performance tests of topic ophthalmic formulations. The OphthalMimic is a 3D-printed device with an artificial lacrimal flow, a cul-de-sac area, a support base, and a simulated cornea comprised of a polymeric membrane containing poly-vinyl alcohol 10 % (w/v), gelatin 2.5 % (w/v), and different proportions of mucin and poloxamer, i.e., 1:1 (M1), 1:2 (M2), and 2:1 (M3) w/v, respectively. The support base is designed to move between 0° and 50° to replicate the movement of an eyelid. We challenged the model by testing the residence performance of poloxamer®407 16 % and poloxamer®407 16 % + chitosan 1 % (PLX16CS10) gels containing fluconazole. The test was conducted with a simulated tear flow of 1.0 mL.min -1 for 5 min. The OphthalMimic successfully distinguished PLX16 and PLX16C10 formulations based on their fluconazole drainage (M1: 65 ± 14 % and 27 ± 10 %; M2: 58 ± 6 % and 38 ± 9 %; M3: 56 ± 5 % and 38 ± 18 %). In conclusion, the OphthalMimic is a promising tool for comparing the animal-free performance of ophthalmic formulations.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: 3D-printed device; Drug delivery; Ocular delivery; Polymeric membrane; Retention time
المشرفين على المادة: 106392-12-5 (Poloxamer)
0 (Ophthalmic Solutions)
8VZV102JFY (Fluconazole)
9012-76-4 (Chitosan)
9000-70-8 (Gelatin)
تواريخ الأحداث: Date Created: 20240517 Date Completed: 20240613 Latest Revision: 20240613
رمز التحديث: 20240614
DOI: 10.1016/j.ymeth.2024.05.005
PMID: 38759909
قاعدة البيانات: MEDLINE
الوصف
تدمد:1095-9130
DOI:10.1016/j.ymeth.2024.05.005