دورية أكاديمية
Preadipocytes potentiate melanoma progression and M2 macrophage polarization in the tumor microenvironment.
| العنوان: | Preadipocytes potentiate melanoma progression and M2 macrophage polarization in the tumor microenvironment. |
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| المؤلفون: | Jeon TJ; Department of Global Innovative Drugs, Graduate School of Chung-Ang University, Seoul, 06974, South Korea., Kim OH; Department of Anatomy and Cell Biology, College of Medicine, Chung-Ang University, Seoul, 06974, South Korea., Kang H; Department of Global Innovative Drugs, Graduate School of Chung-Ang University, Seoul, 06974, South Korea., Lee HJ; Department of Global Innovative Drugs, Graduate School of Chung-Ang University, Seoul, 06974, South Korea; Department of Anatomy and Cell Biology, College of Medicine, Chung-Ang University, Seoul, 06974, South Korea. Electronic address: pluto38@cau.ac.kr. |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Aug 20; Vol. 721, pp. 150129. Date of Electronic Publication: 2024 May 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Tumor Microenvironment* , Macrophages*/metabolism , Macrophages*/pathology , Macrophages*/drug effects , Adipocytes*/metabolism , Adipocytes*/pathology , Adipocytes*/drug effects , Melanoma, Experimental*/pathology , Melanoma, Experimental*/metabolism , Exosomes*/metabolism, Animals ; Mice ; RAW 264.7 Cells ; Coculture Techniques ; Disease Progression ; 3T3-L1 Cells ; Benzylidene Compounds/pharmacology ; Aniline Compounds/pharmacology ; Cell Proliferation/drug effects ; Melanoma/pathology ; Melanoma/metabolism ; Skin Neoplasms/pathology ; Skin Neoplasms/metabolism ; Cell Line, Tumor ; MicroRNAs/metabolism ; MicroRNAs/genetics |
| مستخلص: | Melanoma, the deadliest skin cancer, originates from epidermal melanocytes. The influence of preadipocytes on melanoma is less understood. We co-cultured mouse melanoma B16 cells with 3T3L1 preadipocytes to form mixed spheroids and observed increased melanoma proliferation and growth compared to B16-only spheroids. Metastasis-related proteins YAP, TAZ, and PD-L1 levels were also higher in mixed spheroids. Treatment with exosome inhibitor GW4869 halted melanoma growth and reduced expression of these proteins, suggesting exosomal crosstalk between B16 and 3T3L1 cells. MiR-155 expression was significantly higher in mixed spheroids, and GW4869 reduced its levels. Additionally, co-culturing with Raw264.7 macrophage cells increased M2 markers IL-4 and CD206 in Raw264.7 cells, effects that were diminished by GW4869. These results indicate that preadipocytes may enhance melanoma progression and metastasis via exosomal interactions. Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests or personal relationships that may have influenced the work reported in this study. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Melanoma; Preadipocytes; Tumor microenvironment; miR-155 |
| المشرفين على المادة: | 0 (GW 4869) 0 (Benzylidene Compounds) 0 (Aniline Compounds) 0 (MicroRNAs) |
| تواريخ الأحداث: | Date Created: 20240519 Date Completed: 20240606 Latest Revision: 20240606 |
| رمز التحديث: | 20240607 |
| DOI: | 10.1016/j.bbrc.2024.150129 |
| PMID: | 38762933 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2104 |
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| DOI: | 10.1016/j.bbrc.2024.150129 |