دورية أكاديمية
أعرض في EDS

17β-estradiol and methylprednisolone association as a therapeutic option to modulate lung inflammation in brain-dead female rats.

التفاصيل البيبلوغرافية
العنوان: 17β-estradiol and methylprednisolone association as a therapeutic option to modulate lung inflammation in brain-dead female rats.
المؤلفون: Vidal-Dos-Santos M; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.; Department of Surgery, University Medical Centre Groningen, University of Groningen, Groningen, Netherlands., Anunciação LF; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Armstrong-Jr R; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Ricardo-da-Silva FY; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Ramos IYT; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Correia CJ; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Moreira LFP; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Leuvenink HGD; Department of Surgery, University Medical Centre Groningen, University of Groningen, Groningen, Netherlands., Breithaupt-Faloppa AC; Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
المصدر: Frontiers in immunology [Front Immunol] 2024 May 03; Vol. 15, pp. 1375943. Date of Electronic Publication: 2024 May 03 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Lausanne : Frontiers Research Foundation]
مواضيع طبية MeSH: Estradiol*/pharmacology , Methylprednisolone*/pharmacology , Rats, Wistar* , Brain Death* , Pneumonia*/drug therapy , Pneumonia*/metabolism, Animals ; Female ; Rats ; Cytokines/metabolism ; Lung/drug effects ; Lung/pathology ; Lung/metabolism ; Lung/immunology ; Disease Models, Animal ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use
مستخلص: Introduction: Brain death (BD) is known to compromise graft quality by causing hemodynamic, metabolic, and hormonal changes. The abrupt reduction of female sex hormones after BD was associated with increased lung inflammation. The use of both corticoids and estradiol independently has presented positive results in modulating BD-induced inflammatory response. However, studies have shown that for females the presence of both estrogen and corticoids is necessary to ensure adequate immune response. In that sense, this study aims to investigate how the association of methylprednisolone (MP) and estradiol (E2) could modulate the lung inflammation triggered by BD in female rats.
Methods: Female Wistar rats (8 weeks) were divided into four groups: sham (animals submitted to the surgical process, without induction of BD), BD (animals submitted to BD), MP/E2 (animals submitted to BD that received MP and E2 treatment 3h after BD induction) and MP (animals submitted to BD that received MP treatment 3h after BD induction).
Results: Hemodynamics, systemic and local quantification of IL-6, IL-1β, VEGF, and TNF-α, leukocyte infiltration to the lung parenchyma and airways, and adhesion molecule expression were analyzed. After treatment, MP/E2 association was able to reinstate mean arterial pressure to levels close to Sham animals ( p <0.05). BD increased leukocyte infiltration to the airways and MP/E2 was able to reduce the number of cells ( p =0.0139). Also, the associated treatment modulated the vasculature by reducing the expression of VEGF ( p =0.0616) and maintaining eNOS levels ( p =0.004) in lung tissue.
Discussion: Data presented in this study show that the association between corticoids and estradiol could represent a better treatment strategy for lung inflammation in the female BD donor by presenting a positive effect in the hemodynamic management of the donor, as well as by reducing infiltrated leukocyte to the airways and release of inflammatory markers in the short and long term.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Vidal-dos-Santos, Anunciação, Armstrong-Jr, Ricardo-da-Silva, Ramos, Correia, Moreira, Leuvenink and Breithaupt-Faloppa.)
References: Front Immunol. 2021 Aug 16;12:691957. (PMID: 34484188)
Cell Res. 2006 Jun;16(6):548-58. (PMID: 16775626)
Nat Rev Immunol. 2017 Apr;17(4):233-247. (PMID: 28192415)
J Immunol. 2011 Apr 1;186(7):4354-60. (PMID: 21357268)
Am J Transplant. 2016 Jan;16(1):33-43. (PMID: 26366523)
Lancet. 1999 Apr 24;353(9162):1412-3. (PMID: 10227229)
Front Pharmacol. 2021 Feb 22;12:587003. (PMID: 33692687)
Anesthesiology. 2010 May;112(5):1204-10. (PMID: 20395825)
Inflammation. 2018 Aug;41(4):1488-1497. (PMID: 29737476)
Endocr Rev. 2007 Aug;28(5):521-74. (PMID: 17640948)
J Heart Lung Transplant. 2018 Nov;37(11):1381-1387. (PMID: 30139547)
Crit Care Med. 1996 Aug;24(8):1352-9. (PMID: 8706491)
Transplantation. 2021 Apr 1;105(4):775-784. (PMID: 33031230)
Endocrinology. 1992 Sep;131(3):1261-9. (PMID: 1324155)
FEBS Lett. 2001 Aug 3;502(3):103-8. (PMID: 11583108)
Nat Rev Immunol. 2021 Jan;21(1):49-64. (PMID: 33214719)
Intensive Care Med. 2019 Mar;45(3):343-353. (PMID: 30741327)
Biochem Biophys Res Commun. 2023 May 21;657:108-118. (PMID: 37002984)
Crit Care Med. 1990 Jul;18(7):702-8. (PMID: 2194745)
J Clin Invest. 1993 Oct;92(4):1896-902. (PMID: 8408641)
Respir Res. 2000;1(3):136-40. (PMID: 11667977)
Clinics (Sao Paulo). 2021 Aug 16;76:e3042. (PMID: 34406272)
Mol Cell Endocrinol. 2002 Nov 29;197(1-2):35-44. (PMID: 12431793)
Taiwan J Ophthalmol. 2019 Dec 13;9(4):233-242. (PMID: 31942428)
Crit Care Med. 2008 Jun;36(6):1810-6. (PMID: 18496370)
Am J Respir Crit Care Med. 2013 Jun 15;187(12):1349-59. (PMID: 23590269)
Rom J Morphol Embryol. 2018;59(2):455-467. (PMID: 30173249)
Crit Care Clin. 2020 Apr;36(2):307-321. (PMID: 32172815)
J Clin Invest. 1999 Feb;103(3):401-6. (PMID: 9927501)
Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10972-7. (PMID: 10995484)
Mol Pharmacol. 2007 Jan;71(1):132-44. (PMID: 17035596)
Transplantation. 1987 Jun;43(6):852-4. (PMID: 3296351)
Front Immunol. 2021 Jul 21;12:704173. (PMID: 34367163)
J Surg Res. 2016 Feb;200(2):714-21. (PMID: 26547667)
Transplantation. 2003 Feb 27;75(4):482-7. (PMID: 12605114)
Nucleic Acids Res. 2013 Apr;41(7):4036-48. (PMID: 23444138)
Acta Anaesthesiol Scand. 2009 Nov;53(10):1239-50. (PMID: 19681785)
Transplantation. 2020 Sep;104(9):1862-1868. (PMID: 32345867)
Med Res Rev. 2020 Jan;40(1):158-189. (PMID: 31192483)
Med Hypotheses. 2013 Dec;81(6):1078-82. (PMID: 24199951)
Transpl Int. 2020 Mar;33(3):279-287. (PMID: 31701582)
Ann Transl Med. 2021 Jul;9(14):1125. (PMID: 34430566)
Transpl Int. 2020 Oct;33(10):1312-1321. (PMID: 32621784)
Mol Cell Neurosci. 1993 Apr;4(2):191-8. (PMID: 19912922)
فهرسة مساهمة: Keywords: 17β-estradiol; brain death; females; lung; methylprednisolone
تواريخ الأحداث: Date Created: 20240520 Date Completed: 20240520 Latest Revision: 20240521
رمز التحديث: 20240521
مُعرف محوري في PubMed: PMC11099279
DOI: 10.3389/fimmu.2024.1375943
PMID: 38765005
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-3224
DOI:10.3389/fimmu.2024.1375943