دورية أكاديمية
Structure-based characterization and compound identification of the wild-type THF class-II riboswitch.
| العنوان: | Structure-based characterization and compound identification of the wild-type THF class-II riboswitch. |
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| المؤلفون: | Li C; Life Sciences Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang Key Laboratory of Biotherapy, Zhejiang University, Hangzhou 310058, China., Xu X; The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen 518033, China., Geng Z; Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China., Zheng L; College of Life Sciences, Anhui Normal University, Wuhu 241000 Anhui, China., Song Q; Life Sciences Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang Key Laboratory of Biotherapy, Zhejiang University, Hangzhou 310058, China., Shen X; Life Sciences Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang Key Laboratory of Biotherapy, Zhejiang University, Hangzhou 310058, China., Wu J; State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China., Zhao J; State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China., Li H; Life Sciences Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang Key Laboratory of Biotherapy, Zhejiang University, Hangzhou 310058, China., He M; Life Sciences Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang Key Laboratory of Biotherapy, Zhejiang University, Hangzhou 310058, China., Tai X; Life Sciences Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang Key Laboratory of Biotherapy, Zhejiang University, Hangzhou 310058, China., Zhang L; Life Sciences Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang Key Laboratory of Biotherapy, Zhejiang University, Hangzhou 310058, China., Ma J; State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China., Dong Y; Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.; University of Chinese Academy of Sciences, Beijing 100049, China., Ren A; Life Sciences Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang Key Laboratory of Biotherapy, Zhejiang University, Hangzhou 310058, China. |
| المصدر: | Nucleic acids research [Nucleic Acids Res] 2024 Aug 12; Vol. 52 (14), pp. 8454-8465. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1992- : Oxford : Oxford University Press Original Publication: London, Information Retrieval ltd. |
| مواضيع طبية MeSH: | Riboswitch*/genetics , Tetrahydrofolates*/chemistry , Tetrahydrofolates*/metabolism , Nucleic Acid Conformation*, Ligands ; Models, Molecular ; RNA Folding ; Nucleotide Motifs ; Mutation |
| مستخلص: | Riboswitches are conserved regulatory RNA elements participating in various metabolic pathways. Recently, a novel RNA motif known as the folE RNA motif was discovered upstream of folE genes. It specifically senses tetrahydrofolate (THF) and is therefore termed THF-II riboswitch. To unravel the ligand recognition mechanism of this newly discovered riboswitch and decipher the underlying principles governing its tertiary folding, we determined both the free-form and bound-form THF-II riboswitch in the wild-type sequences. Combining structural information and isothermal titration calorimetry (ITC) binding assays on structure-based mutants, we successfully elucidated the significant long-range interactions governing the function of THF-II riboswitch and identified additional compounds, including alternative natural metabolites and potential lead compounds for drug discovery, that interact with THF-II riboswitch. Our structural research on the ligand recognition mechanism of the THF-II riboswitch not only paves the way for identification of compounds targeting riboswitches, but also facilitates the exploration of THF analogs in diverse biological contexts or for therapeutic applications. (© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.) |
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| معلومات مُعتمدة: | 32325029 National Natural Science Foundation of China; 2023YFC2604300 National Key Research and Development Project of China |
| المشرفين على المادة: | 0 (Riboswitch) 0 (Tetrahydrofolates) 0 (Ligands) 43ZWB253H4 (5,6,7,8-tetrahydrofolic acid) |
| تواريخ الأحداث: | Date Created: 20240520 Date Completed: 20240811 Latest Revision: 20240822 |
| رمز التحديث: | 20240822 |
| مُعرف محوري في PubMed: | PMC11317127 |
| DOI: | 10.1093/nar/gkae377 |
| PMID: | 38769061 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1362-4962 |
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| DOI: | 10.1093/nar/gkae377 |