دورية أكاديمية
Anti-obesity effects of a standardized ethanol extract of Eisenia bicyclis by regulating the AMPK signaling pathway in 3T3-L1 cells and HFD-induced mice.
| العنوان: | Anti-obesity effects of a standardized ethanol extract of Eisenia bicyclis by regulating the AMPK signaling pathway in 3T3-L1 cells and HFD-induced mice. |
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| المؤلفون: | Yoon YS; Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul, 02447, Republic of Korea. ktlee@khu.ac.kr.; Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea., Chung KS; Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul, 02447, Republic of Korea. ktlee@khu.ac.kr., Lee SY; Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul, 02447, Republic of Korea. ktlee@khu.ac.kr., Heo SW; Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul, 02447, Republic of Korea. ktlee@khu.ac.kr.; Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea., Kim YR; Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul, 02447, Republic of Korea. ktlee@khu.ac.kr.; Department of Fundamental Pharmaceutical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea., Lee JK; Department of Fundamental Pharmaceutical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.; Department of Pharmacy, College of Pharmacy, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea., Kim H; Department of New Material Development, COSMAXBIO, Gyeonggi, 13486, Republic of Korea., Park S; Department of New Material Development, COSMAXBIO, Gyeonggi, 13486, Republic of Korea., Shin YK; Department of New Material Development, COSMAXBIO, Gyeonggi, 13486, Republic of Korea., Lee KT; Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul, 02447, Republic of Korea. ktlee@khu.ac.kr.; Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.; Department of Fundamental Pharmaceutical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea. |
| المصدر: | Food & function [Food Funct] 2024 Jun 17; Vol. 15 (12), pp. 6424-6437. Date of Electronic Publication: 2024 Jun 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101549033 Publication Model: Electronic Cited Medium: Internet ISSN: 2042-650X (Electronic) Linking ISSN: 20426496 NLM ISO Abbreviation: Food Funct Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge : Royal Society of Chemistry |
| مواضيع طبية MeSH: | Diet, High-Fat*/adverse effects , 3T3-L1 Cells* , Plant Extracts*/pharmacology , AMP-Activated Protein Kinases*/metabolism , AMP-Activated Protein Kinases*/genetics , Anti-Obesity Agents*/pharmacology , Mice, Inbred C57BL* , Obesity*/drug therapy , Obesity*/metabolism , Signal Transduction*/drug effects, Animals ; Mice ; Male ; Sterol Regulatory Element Binding Protein 1/metabolism ; Sterol Regulatory Element Binding Protein 1/genetics ; Adipogenesis/drug effects ; PPAR gamma/metabolism ; PPAR gamma/genetics ; CCAAT-Enhancer-Binding Protein-alpha/metabolism ; CCAAT-Enhancer-Binding Protein-alpha/genetics ; Adipocytes/drug effects ; Adipocytes/metabolism ; Edible Seaweeds ; Kelp |
| مستخلص: | Obesity requires treatment to mitigate the potential development of further metabolic disorders, including diabetes, hyperlipidemia, tumor growth, and non-alcoholic fatty liver disease. We investigated the anti-obesity effect of a 30% ethanol extract of Eisenia bicyclis (Kjellman) Setchell (EEB) on 3T3-L1 preadipocytes and high-fat diet (HFD)-induced obese C57BL/6 mice. Adipogenesis transcription factors including peroxisome proliferator-activated receptor (PPAR)γ, CCAAT/enhancer-binding protein-alpha (C/EBPα), and sterol regulatory element-binding protein-1 (SREBP-1) were ameliorated through the AMP-activated protein kinase (AMPK) pathway by EEB treatment in differentiated 3T3-L1 cells. EEB attenuated mitotic clonal expansion by upregulating cyclin-dependent kinase inhibitors (CDKIs) while downregulating cyclins and CDKs. In HFD-fed mice, EEB significantly decreased the total body weight, fat tissue weight, and fat in the tissue. The protein expression of PPARγ, C/EBPα, and SREBP-1 was increased in the subcutaneous fat and liver tissues, while EEB decreased the expression levels of these transcription factors. EEB also inhibited lipogenesis by downregulating acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) expression in the subcutaneous fat and liver tissues. Moreover, the phosphorylation of AMPK and ACC was downregulated in the HFD-induced mouse group, whereas the administration of EEB improved AMPK and ACC phosphorylation; thus, EEB treatment may be related to the AMPK pathway. Histological analysis showed that EEB reduced the adipocyte size and fat accumulation in subcutaneous fat and liver tissues, respectively. EEB promotes thermogenesis in brown adipose tissue and improves insulin and leptin levels and blood lipid profiles. Our results suggest that EEB could be used as a potential agent to prevent obesity. |
| المشرفين على المادة: | 0 (Plant Extracts) EC 2.7.11.31 (AMP-Activated Protein Kinases) 0 (Anti-Obesity Agents) 0 (Sterol Regulatory Element Binding Protein 1) 0 (PPAR gamma) 0 (CCAAT-Enhancer-Binding Protein-alpha) |
| SCR Organism: | Eisenia brown algae |
| تواريخ الأحداث: | Date Created: 20240521 Date Completed: 20240617 Latest Revision: 20240617 |
| رمز التحديث: | 20240619 |
| DOI: | 10.1039/d4fo00759j |
| PMID: | 38771619 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2042-650X |
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| DOI: | 10.1039/d4fo00759j |