دورية أكاديمية
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Clinical evaluation of a novel digital microfluidic based point-of-care test platform for detection of SARS-Cov-2 and influenza A/B.

التفاصيل البيبلوغرافية
العنوان: Clinical evaluation of a novel digital microfluidic based point-of-care test platform for detection of SARS-Cov-2 and influenza A/B.
المؤلفون: Huang H; Zhuhai Center for Disease Control and Prevention, Zhuhai, 519087, China., Long D; Zhuhai Center for Disease Control and Prevention, Zhuhai, 519087, China., Lin Y; Zhuhai Center for Disease Control and Prevention, Zhuhai, 519087, China., Dong C; School of Intelligent Systems Science and Engineering/JNU-Industry School of Artificial Intelligence, Jinan University, Zhuhai, 519000, China; Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, China., Huang W; Zhuhai Center for Disease Control and Prevention, Zhuhai, 519087, China., Zhang M; Digifluidic Biotech Inc., Zhuhai, 519000, China., Wan L; Livzon Diagnostics Inc., Zhuhai, 519000, China., Gou H; Livzon Diagnostics Inc., Zhuhai, 519000, China., Chen T; Digifluidic Biotech Inc., Zhuhai, 519000, China., Li F; Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, China. Electronic address: fl98@njit.edu.
المصدر: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2024 Aug; Vol. 173, pp. 105688. Date of Electronic Publication: 2024 May 14.
نوع المنشور: Journal Article; Evaluation Study
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 9815671 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-5967 (Electronic) Linking ISSN: 13866532 NLM ISO Abbreviation: J Clin Virol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier Science, c1998-
مواضيع طبية MeSH: Influenza, Human*/diagnosis , Influenza, Human*/virology , COVID-19*/diagnosis , Point-of-Care Testing* , SARS-CoV-2*/isolation & purification , SARS-CoV-2*/genetics , Sensitivity and Specificity* , Influenza B virus*/isolation & purification , Influenza B virus*/genetics , Influenza A virus*/isolation & purification , Influenza A virus*/genetics, Humans ; Microfluidics/methods ; Microfluidics/instrumentation ; Point-of-Care Systems
مستخلص: Respiratory pathogens, such as SARS-CoV-2 and influenza A/B, can cause severe illnesses in susceptible individuals. This research evaluated a novel digital microfluidic point-of-care testing platform designed to detect 23 pathogens, comparing its performance to conventional laboratory-based nucleic acid tests. The platform integrates nucleic acid extraction and amplification processes for rapid detection with only 2 min of hands-on time. Performance assays demonstrated that the platform has high sensitivity (87 %-100 %) and specificity (99 %-100 %) for the detection of the evaluated 3 viruses. Additionally, the platform can be adapted for the detection of other respiratory pathogens, aiding in the early diagnosis of respiratory diseases, identifying the source of an outbreak or epidemic, and curbing the spread of the disease.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: For this study, funding acquired from government financial appropriations. Coauthors LW, HG, TC, MZ are affiliated with Digifluidic Inc. and Livzon Inc., which may have potential conflicts of interest. They provided technical support and problem-solving assistance during the test.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Digital microfluidic platform; Influenza A/B, Nucleic acid testing; Point-of-care testing; Respiratory pathogens; SARS-Cov-2
تواريخ الأحداث: Date Created: 20240522 Date Completed: 20240609 Latest Revision: 20240609
رمز التحديث: 20240610
DOI: 10.1016/j.jcv.2024.105688
PMID: 38776575
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-5967
DOI:10.1016/j.jcv.2024.105688