دورية أكاديمية
Competitive fitness of asymptomatic bacteriuria E. coli strain 83972 against uropathogens in human urine.
| العنوان: | Competitive fitness of asymptomatic bacteriuria E. coli strain 83972 against uropathogens in human urine. |
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| المؤلفون: | George I; Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA., Kalairaj MS; Department of Biomedical Engineering, Texas A&M University, College Station, Texas, USA., Zimmern PE; Department of Urology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Ware TH; Department of Biomedical Engineering, Texas A&M University, College Station, Texas, USA.; Department of Materials Science and Engineering, Texas A&M University, College Station, Texas, USA., Subashchandrabose S; Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA. |
| المصدر: | Infection and immunity [Infect Immun] 2024 Jun 11; Vol. 92 (6), pp. e0017324. Date of Electronic Publication: 2024 May 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0246127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5522 (Electronic) Linking ISSN: 00199567 NLM ISO Abbreviation: Infect Immun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : American Society For Microbiology Original Publication: [Bethesda, Md.] American Society for Microbiology. |
| مواضيع طبية MeSH: | Bacteriuria*/microbiology , Uropathogenic Escherichia coli*/genetics , Uropathogenic Escherichia coli*/drug effects , Escherichia coli Infections*/microbiology , Escherichia coli Infections*/urine , Urinary Tract Infections*/microbiology, Humans ; Animals ; Mice ; Female ; Urine/microbiology ; Escherichia coli/genetics ; Escherichia coli/drug effects |
| مستخلص: | Urinary tract infection (UTI) is one of the most common bacterial infections worldwide. The main causative agent of UTI is uropathogenic Escherichia coli (UPEC). There is an immediate need for novel prophylactic and treatment strategies against UTI because of the increasing incidence of antimicrobial resistance among uropathogens. ABU 83972, an asymptomatic bacteriuria-causing E. coli strain , prevents UTI by suppressing the colonization of UPEC. However, the nature of competition and growth repression of UPEC by ABU 83972 is unclear and is the subject of our investigation. Here, we characterized the growth kinetics of ABU 83972 and uropathogens in human urine and laboratory media. Next, we performed a series of competitive co-culture experiments where ABU 83972 and uropathogens were inoculated at a 1:1 ratio in human urine and in various media, and their relative abundance was determined. In human urine, ABU 83972 outcompeted UPEC and additional uropathogens, reaching up to 90% of the total population after 24 hours of incubation. In contrast, UPEC outcompeted ABU 83972 in LB and M9 minimal media and exhibited superior colonization than ABU 83972 in the mouse urinary bladder. Since engineered living materials (ELMs) can be used to retain an organism of interest in a particular location, we developed ABU 83972-containing ELMs that effectively outcompeted UPEC in human urine. In summary, our work establishes that ABU 83972 outcompetes UPEC in a milieu- and cell-density-dependent manner, highlighting the importance of the metabolites and nutrients found in the human urine as determinants of the competitive fitness of ABU 83972. Competing Interests: The authors declare no conflict of interest. |
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| معلومات مُعتمدة: | R56 EB032395 United States EB NIBIB NIH HHS; R21 AI166162 United States AI NIAID NIH HHS; HHS | NIH | National Institute of Biomedical Imaging and Bioengineering (NIBIB); HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK); R01 DK114224 United States DK NIDDK NIH HHS; HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) |
| فهرسة مساهمة: | Keywords: ABU; ABU 83972; ELM; UPEC; UTI; uropathogens |
| تواريخ الأحداث: | Date Created: 20240523 Date Completed: 20240611 Latest Revision: 20240714 |
| رمز التحديث: | 20240714 |
| مُعرف محوري في PubMed: | PMC11237815 |
| DOI: | 10.1128/iai.00173-24 |
| PMID: | 38780216 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5522 |
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| DOI: | 10.1128/iai.00173-24 |