دورية أكاديمية
أعرض في EDS

Transcriptional regulation of living materials via extracellular electron transfer.

التفاصيل البيبلوغرافية
العنوان: Transcriptional regulation of living materials via extracellular electron transfer.
المؤلفون: Graham AJ; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., Partipilo G; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., Dundas CM; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., Miniel Mahfoud IE; Interdisciplinary Life Sciences Graduate Program, University of Texas at Austin, Austin, TX, USA., Halwachs KN; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., Holwerda AJ; Interdisciplinary Life Sciences Graduate Program, University of Texas at Austin, Austin, TX, USA., Simmons TR; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., FitzSimons TM; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., Coleman SM; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., Rinehart R; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., Chiu D; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., Tyndall AE; Department of Chemical and Biological Engineering, Colorado School of Mines, Golden, CO, USA., Sajbel KC; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., Rosales AM; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., Keitz BK; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA. keitz@utexas.edu.
المصدر: Nature chemical biology [Nat Chem Biol] 2024 May 23. Date of Electronic Publication: 2024 May 23.
Publication Model: Ahead of Print
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101231976 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4469 (Electronic) Linking ISSN: 15524450 NLM ISO Abbreviation: Nat Chem Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, NY : Nature Pub. Group, [2005]-
مستخلص: Engineered living materials combine the advantages of biological and synthetic systems by leveraging genetic and metabolic programming to control material-wide properties. Here, we demonstrate that extracellular electron transfer (EET), a microbial respiration process, can serve as a tunable bridge between live cell metabolism and synthetic material properties. In this system, EET flux from Shewanella oneidensis to a copper catalyst controls hydrogel cross-linking via two distinct chemistries to form living synthetic polymer networks. We first demonstrate that synthetic biology-inspired design rules derived from fluorescence parameterization can be applied toward EET-based regulation of polymer network mechanics. We then program transcriptional Boolean logic gates to govern EET gene expression, which enables design of computational polymer networks that mechanically respond to combinations of molecular inputs. Finally, we control fibroblast morphology using EET as a bridge for programmed material properties. Our results demonstrate how rational genetic circuit design can emulate physiological behavior in engineered living materials.
(© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.)
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معلومات مُعتمدة: FA9550-20-1-0088 United States Department of Defense | United States Air Force | AFMC | Air Force Office of Scientific Research (AF Office of Scientific Research); 1944334 National Science Foundation (NSF); DMR-1720595 National Science Foundation (NSF); ECCS-1542159 National Science Foundation (NSF); 1015895 Burroughs Wellcome Fund (BWF); F-1929 Welch Foundation; R35GM133640 U.S. Department of Health & Human Services | National Institutes of Health (NIH)
تواريخ الأحداث: Date Created: 20240523 Latest Revision: 20240717
رمز التحديث: 20240718
DOI: 10.1038/s41589-024-01628-y
PMID: 38783133
قاعدة البيانات: MEDLINE
الوصف
تدمد:1552-4469
DOI:10.1038/s41589-024-01628-y