دورية أكاديمية
Pyrogallol intermediates elicit beta-amyloid secretion via radical formation and alterations in intracellular trafficking, distinct from pyrogallol-generated superoxide.
| العنوان: | Pyrogallol intermediates elicit beta-amyloid secretion via radical formation and alterations in intracellular trafficking, distinct from pyrogallol-generated superoxide. |
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| المؤلفون: | Jang BG; Institute of Epilepsy Research, College of Medicine, Hallym University, Chuncheon, 24252, South Korea., Choi B; Institute of Epilepsy Research, College of Medicine, Hallym University, Chuncheon, 24252, South Korea., Kim MJ; Institute of Epilepsy Research, College of Medicine, Hallym University, Chuncheon, 24252, South Korea; Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon, 24252, South Korea. Electronic address: zolim@hallym.ac.kr. |
| المصدر: | Redox biology [Redox Biol] 2024 Jul; Vol. 73, pp. 103180. Date of Electronic Publication: 2024 May 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier, B.V Country of Publication: Netherlands NLM ID: 101605639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-2317 (Electronic) Linking ISSN: 22132317 NLM ISO Abbreviation: Redox Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam]: Elsevier, B.V., [2013]- |
| مواضيع طبية MeSH: | Amyloid beta-Peptides*/metabolism , Pyrogallol*/pharmacology , Pyrogallol*/analogs & derivatives , Superoxides*/metabolism , Alzheimer Disease*/metabolism , Alzheimer Disease*/etiology , Alzheimer Disease*/pathology, Humans ; Animals ; Astrocytes/metabolism ; Exocytosis ; Endocytosis ; Superoxide Dismutase/metabolism ; Cyclic N-Oxides/pharmacology |
| مستخلص: | This study unveils a novel role of pyrogallol (PG), a recognized superoxide generator, in inducing beta-amyloid (Aβ) secretion in an Alzheimer's disease (AD) cellular model. Contrary to expectations, the analysis of dihydroethidium fluorescence and UV-VIS spectrum scanning reveals that Aβ secretion arises from PG reaction intermediates rather than superoxide or other by-products. Investigation into Aβ secretion mechanisms identifies dynasore-dependent endocytosis and BFA-dependent exocytosis as independent pathways, regulated by tiron, tempol, and superoxide dismutase. Cell-type specificity is observed, with 293sw cells showing both pathways, while H4sw cells and primary astrocytes from an AD animal model exclusively exhibit the Aβ exocytosis pathway. This exploration contributes to understanding PG's chemical reactions and provides insights into the interplay between environmental factors, free radicals, and AD, linking occupational PG exposure to AD risk as reported in the literature. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| معلومات مُعتمدة: | 2022R1A2C1003010 National Research Foundation of Korea(NRF) |
| فهرسة مساهمة: | Keywords: Alzheimer's disease; Beta-amyloid; Free radical; Intracellular trafficking; Pyrogallol |
| المشرفين على المادة: | 0 (Amyloid beta-Peptides) 01Y4A2QXY0 (Pyrogallol) 11062-77-4 (Superoxides) EC 1.15.1.1 (Superoxide Dismutase) 0 (Cyclic N-Oxides) |
| تواريخ الأحداث: | Date Created: 20240525 Date Completed: 20240612 Latest Revision: 20240918 |
| رمز التحديث: | 20240919 |
| مُعرف محوري في PubMed: | PMC11140794 |
| DOI: | 10.1016/j.redox.2024.103180 |
| PMID: | 38795546 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2213-2317 |
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| DOI: | 10.1016/j.redox.2024.103180 |