دورية أكاديمية
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Detection of KPC enzyme by MALDI-TOF MS from bacteria impregnated in filter paper.

التفاصيل البيبلوغرافية
العنوان: Detection of KPC enzyme by MALDI-TOF MS from bacteria impregnated in filter paper.
المؤلفون: Wilhelm CM; Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS) - Hospital de Clinicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil. Electronic address: camilawilhelm@gmail.com., Moreira NK; Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS) - Hospital de Clinicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil., Carneiro MS; Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS) - Hospital de Clinicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil., Wink PL; Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS) - Hospital de Clinicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil., Caierão J; Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil., Barth AL; Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS) - Hospital de Clinicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.
المصدر: Journal of microbiological methods [J Microbiol Methods] 2024 Aug; Vol. 223, pp. 106962. Date of Electronic Publication: 2024 May 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8359 (Electronic) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, The Netherlands : Elsevier Biomedical, c1983-
مواضيع طبية MeSH: Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*/methods , beta-Lactamases*/analysis , beta-Lactamases*/metabolism , Bacterial Proteins*/analysis , Bacterial Proteins*/isolation & purification , Klebsiella pneumoniae*/enzymology , Klebsiella pneumoniae*/isolation & purification, Paper ; Sensitivity and Specificity ; Carbapenems/pharmacology ; Humans ; Enterobacteriaceae/enzymology ; Enterobacteriaceae/isolation & purification ; Anti-Bacterial Agents/pharmacology
مستخلص: The main mechanism that causes resistance to carbapenem, one of the most potent antibiotic available, in Enterobacterales bacterial isolates, is due to Klebsiella pneumoniae carbapenemase (KPC) production by the bacterium. KPC is spread worldwide, requiring laboratories to be capable of identifying this enzyme, however some methods can be expensive for small laboratories, especially in developing countries. Therefore, the development of methods with low cost of reagents for the detection of KPC enzyme is necessary. The objective of this study was to evaluate the detection of KPC enzyme by MALDI-TOF MS from inactivated bacteria impregnated in filter paper. A total of 129 Enterobacterales isolates were impregnated in filter paper, and after 7 days at room temperature, they were subjected to a protein extraction protocol and spectra acquisition, in triplicates, by MALDI-TOF MS. The spectra were evaluated and KPC was identified according to the presence of a peak of 28,712.62 ± 27.80 m/z. Considering the presence of the KPC peak in at least one spectrum of the triplicates, this method presented 60.8% sensitivity and 96.4% specificity. However, considering the presence of KPC peak in at least two spectra of the triplicate, a specificity of 100% was achieved. The detection of KPC enzyme from inactivated bacteria impregnated in filter paper can be used as a method to confirm the presence of KPC, which could be very significant for small laboratories with limited resources.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Biomarker; Filter paper; KPC; MALDI-TOF MS; Resistance
المشرفين على المادة: EC 3.5.2.6 (beta-Lactamases)
EC 3.5.2.6 (carbapenemase)
0 (Bacterial Proteins)
0 (Carbapenems)
0 (Anti-Bacterial Agents)
تواريخ الأحداث: Date Created: 20240525 Date Completed: 20240719 Latest Revision: 20240722
رمز التحديث: 20240722
DOI: 10.1016/j.mimet.2024.106962
PMID: 38795732
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-8359
DOI:10.1016/j.mimet.2024.106962