دورية أكاديمية
Fabrication and characterization of physically crosslinked alginate/chitosan-based hydrogel loaded with neomycin for the treatment of skin infections caused by Staphylococcus aureus.
| العنوان: | Fabrication and characterization of physically crosslinked alginate/chitosan-based hydrogel loaded with neomycin for the treatment of skin infections caused by Staphylococcus aureus. |
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| المؤلفون: | Silva LDS; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil., Vila Nova BG; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil., Sousa CEM; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil., Silva RG; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil., Carvalho LRS; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil., Silva ISS; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil., Moreira PHA; Laboratório de Odontologia, Universidade CEUMA, São Luís 65075-120, MA, Brazil., Cardenas AFM; Laboratório de Odontologia, Universidade CEUMA, São Luís 65075-120, MA, Brazil., Monteiro CA; Laboratório de Pesquisa e Estudo em Microbiologia, Instituto Federal de Educação, Ciência e Tecnologia do Maranhão (IFMA), São Luís 65030-005, Brazil., Tofanello A; Center for Advanced Graphene, Nanomaterials and Nanotechnology Research (MackGraphe), Universidade Presbiteriana Mackenzie, SP, Brazil; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC, SP, Brazil., Garcia W; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC, SP, Brazil., Teixeira CS; Centro de Ciências Agrárias e da Biodiversidade, Universidade Federal do Cariri, CE, Brazil., Nascimento da Silva LC; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil. Electronic address: luiscn.silva@ceuma.com. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2024 Jun; Vol. 271 (Pt 1), pp. 132577. Date of Electronic Publication: 2024 May 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Chitosan*/chemistry , Chitosan*/pharmacology , Alginates*/chemistry , Alginates*/pharmacology , Hydrogels*/chemistry , Hydrogels*/pharmacology , Staphylococcus aureus*/drug effects , Neomycin*/pharmacology , Neomycin*/chemistry , Neomycin*/administration & dosage , Anti-Bacterial Agents*/pharmacology , Anti-Bacterial Agents*/chemistry, Animals ; Mice ; Staphylococcal Infections/drug therapy ; Staphylococcal Skin Infections/drug therapy ; Staphylococcal Skin Infections/microbiology ; Staphylococcal Skin Infections/pathology ; Drug Carriers/chemistry ; Skin/drug effects ; Skin/microbiology |
| مستخلص: | Staphylococcus aureus is a pathogen widely involved in wound infection due to its ability to release several virulence factors that impair the skin healing process, as well as its mechanism of drug resistance. Herein, sodium alginate and chitosan were combined to produce a hydrogel for topical delivery of neomycin to combat S. aureus associated with skin complications. The hydrogel was formulated by combining sodium alginate (50 mg/mL) and chitosan (50 mg/mL) solutions in a ratio of 9:1 (HBase). Neomycin was added to HBase to achieve a concentration of 0.4 mg/mL (HNeo). The incorporation of neomycin into the product was confirmed by scanning electron microscopy, FTIR and TGA analysis. The hydrogels produced are homogeneous, have a high swelling capacity, and show biocompatibility using erythrocytes and fibroblasts as models. The formulations showed physicochemical and pharmacological stability for 60 days at 4 ± 2 °C. HNeo totally inhibited the growth of S. aureus after 4 h. The antimicrobial effects were confirmed using ex vivo (porcine skin) and in vivo (murine) wound infection models. Furthermore, the HNeo-treated mice showed lower severity scores than those treated with HBase. Taken together, the obtained results present a new low-cost bioproduct with promising applications in treating infected wounds. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Biocompatible hydrogel; Infected wounds; Polymeric formulation |
| المشرفين على المادة: | 9012-76-4 (Chitosan) 0 (Alginates) 0 (Hydrogels) I16QD7X297 (Neomycin) 0 (Anti-Bacterial Agents) 0 (Drug Carriers) |
| تواريخ الأحداث: | Date Created: 20240525 Date Completed: 20240619 Latest Revision: 20240619 |
| رمز التحديث: | 20240619 |
| DOI: | 10.1016/j.ijbiomac.2024.132577 |
| PMID: | 38795887 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2024.132577 |