دورية أكاديمية
Molecular taxonomic characterization and infra-specific diversity of entomopathogenic Beauveria bassiana fungi from Argentina.
| العنوان: | Molecular taxonomic characterization and infra-specific diversity of entomopathogenic Beauveria bassiana fungi from Argentina. |
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| المؤلفون: | Manfrino R; IDICAL-Instituto de Investigación de la Cadena Láctea, CONICET-Consejo Nacional de Investigaciones Científicas y Técnicas, INTA-Instituto Nacional de Tecnología Agropecuaria, Rafaela, 2300, Santa Fe, Argentina. Electronic address: manfrino@cepave.edu.ar., Gutierrez A; CEPAVE-Centro de Estudios Parasitológicos y de Vectores, Consejo Nacional de Investigaciones Científicas y Técnicas, UNLP-Universidad Nacional de La Plata, La Plata, 1900, Buenos Aires, Argentina., Ben Gharsa H; Insect-Associated Microorganisms and Microbial Control, Department of Biology, Technische Universität Darmstadt, 64287, Darmstadt, Germany., Schuster C; Insect-Associated Microorganisms and Microbial Control, Department of Biology, Technische Universität Darmstadt, 64287, Darmstadt, Germany., López Lastra C; CEPAVE-Centro de Estudios Parasitológicos y de Vectores, Consejo Nacional de Investigaciones Científicas y Técnicas, UNLP-Universidad Nacional de La Plata, La Plata, 1900, Buenos Aires, Argentina., Leclerque A; Insect-Associated Microorganisms and Microbial Control, Department of Biology, Technische Universität Darmstadt, 64287, Darmstadt, Germany. Electronic address: andreas.leclerque@tu-darmstadt.de. |
| المصدر: | Fungal biology [Fungal Biol] 2024 May; Vol. 128 (3), pp. 1800-1805. Date of Electronic Publication: 2024 Apr 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published by Elsevier, copyright by British Mycological Society Country of Publication: Netherlands NLM ID: 101524465 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-6146 (Print) NLM ISO Abbreviation: Fungal Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Published by Elsevier, copyright by British Mycological Society, c2010- |
| مواضيع طبية MeSH: | Beauveria*/genetics , Beauveria*/classification , Beauveria*/isolation & purification , Phylogeny* , Genetic Variation* , RNA, Ribosomal, 28S*/genetics, Argentina ; Animals ; DNA, Fungal/genetics ; Insecta/microbiology ; Sequence Analysis, DNA ; Molecular Sequence Data ; Introns ; DNA, Ribosomal/genetics ; Cluster Analysis |
| مستخلص: | It has been the aim of this study to molecular-taxonomically identify 15 Beauveria isolates collected from different geographical regions and insect hosts in Argentina and to investigate the levels of inter- and intra-specific diversity across this set of isolates. Based on phylogenetic analyses of EF1A-RPB1-RPB2 concatenated genes and BLOC markers, all Beauveria strains were identify as Beauveria bassiana. Within the B. bassiana clades of both phylogenies, isolates from Argentina were not clustered according to geographic origin or host. The 15 fungal isolates were further analyzed by PCR amplification of the intron insertion hot spot region of the nuclear 28S rRNA encoding sequence. By intron sequence and position, seven different group-I intron combinations termed variants A, B1, B2, C, D, E and F were found in the 15 isolates under study. Variants B1/B2 consisting of a single 28Si2 intron were found in ten isolates, whereas variant A occurred twice and variants C through F were unique across the set of isolates under study. The determination of the different introns and intron combinations in the 28S rRNA gene is a powerful tool for achieving infraspecific differentiation of B. bassiana isolates from Argentina. Competing Interests: Declaration of competing interest none. (Copyright © 2024 British Mycological Society. Published by Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Beauveria; Entomopathogenic fungi; Group-I introns; Intra-specific variation; Molecular taxonomy |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 28S) 0 (DNA, Fungal) 0 (DNA, Ribosomal) |
| تواريخ الأحداث: | Date Created: 20240525 Date Completed: 20240525 Latest Revision: 20240525 |
| رمز التحديث: | 20240527 |
| DOI: | 10.1016/j.funbio.2024.04.003 |
| PMID: | 38796264 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-6146 |
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| DOI: | 10.1016/j.funbio.2024.04.003 |