دورية أكاديمية
أعرض في EDS

Effect of Cannabis sativa L. extracts, phytocannabinoids and their acetylated derivates on the SHSY-5Y neuroblastoma cells' viability and caspases 3/7 activation.

التفاصيل البيبلوغرافية
العنوان: Effect of Cannabis sativa L. extracts, phytocannabinoids and their acetylated derivates on the SHSY-5Y neuroblastoma cells' viability and caspases 3/7 activation.
المؤلفون: Tapia-Tapia E; Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Av. Gran Bretaña 1093, Playa Ancha, 2360102, Valparaíso, Chile.; Escuela de Química y Farmacia, Facultad de Medicina, Universidad Andrés Bello, 2520000, Viña del Mar, Chile., Aránguiz P; Escuela de Ciencias de la Salud. Carrera de Química y Farmacia, Universidad Viña del Mar, 2572007, Viña del Mar, Chile., Diaz R; Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Av. Gran Bretaña 1093, Playa Ancha, 2360102, Valparaíso, Chile.; Centro de Micro-Bioinnovación (CMBi), Universidad de Valparaíso, Av. Gran Bretaña 1093, Playa Ancha, 2360102, Valparaíso, Chile., Espinoza L; Departamento de Química, Universidad Técnica Federico Santa María, Avenida España 1680, 2340000, Valparaíso, Chile., Weinstein-Oppenheimer CR; Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Av. Gran Bretaña 1093, Playa Ancha, 2360102, Valparaíso, Chile. Caroline.weinstein@uv.cl.; Centro de Investigación, Desarrollo e Innovación de Productos Bioactivos (CInBIO), Universidad de Valparaíso, 2360102, Valparaíso, Chile. Caroline.weinstein@uv.cl., Cuellar M; Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Av. Gran Bretaña 1093, Playa Ancha, 2360102, Valparaíso, Chile. Mauricio.cuellar@uv.cl.; Centro de Investigación, Desarrollo e Innovación de Productos Bioactivos (CInBIO), Universidad de Valparaíso, 2360102, Valparaíso, Chile. Mauricio.cuellar@uv.cl.
المصدر: Biological research [Biol Res] 2024 May 27; Vol. 57 (1), pp. 33. Date of Electronic Publication: 2024 May 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Biomed Central, Ltd Country of Publication: England NLM ID: 9308271 Publication Model: Electronic Cited Medium: Internet ISSN: 0717-6287 (Electronic) Linking ISSN: 07169760 NLM ISO Abbreviation: Biol Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 2014- : London : Biomed Central, Ltd
Original Publication: Santiago : Society of Biology of Chile, [1992-
مواضيع طبية MeSH: Cannabis*/chemistry , Plant Extracts*/pharmacology , Plant Extracts*/chemistry , Neuroblastoma*/drug therapy , Cell Survival*/drug effects , Caspase 3*/metabolism , Caspase 3*/drug effects , Cannabinoids*/pharmacology , Cannabinoids*/chemistry, Humans ; Cell Line, Tumor ; Caspase 7/metabolism ; Apoptosis/drug effects ; Acetylation/drug effects ; Chromatography, High Pressure Liquid
مستخلص: Background: There is a need for novel treatments for neuroblastoma, despite the emergence of new biological and immune treatments, since refractory pediatric neuroblastoma is still a medical challenge. Phyto cannabinoids and their hemisynthetic derivatives have shown evidence supporting their anticancer potential. The aim of this research was to examine Phytocannabinoids or hemisynthetic cannabinoids, which reduce the SHSY-5Y, neuroblastoma cell line's viability.
Methods: Hexane and acetyl acetate extracts were produced starting with Cannabis sativa L. as raw material, then, 9-tetrahidrocannabinol, its acid counterpart and CBN were isolated. In addition, acetylated derivatives of THC and CBN were synthesized. The identification and purity of the chemicals was determined by High Performance Liquid Chromatography and 1 H y 13 C Magnetic Nuclear Resonance. Then, the capacity to affect the viability of SHSY-5Y, a neuroblastoma cell line, was examined using the resazurin method. Finally, to gain insight into the mechanism of action of the extracts, phytocannabinoids and acetylated derivatives on the examined cells, a caspase 3/7 determination was performed on cells exposed to these compounds.
Results: The structure and purity of the isolated compounds was demonstrated. The extracts, the phytocannabinoids and their acetylated counterparts inhibited the viability of the SHSY 5Y cells, being CBN the most potent of all the tested molecules with an inhibitory concentration of 50 percent of 9.5 µM.
Conclusion: Each of the evaluated molecules exhibited the capacity to activate caspases 3/7, indicating that at least in part, the cytotoxicity of the tested phytocannabinoids and their hemi-synthetic derivatives is mediated by apoptosis.
(© 2024. The Author(s).)
References: Cell Death Differ. 2011 Jul;18(7):1099-111. (PMID: 21475304)
Immunity. 2019 Jun 18;50(6):1352-1364. (PMID: 31216460)
J Nat Prod. 2020 Mar 27;83(3):770-803. (PMID: 32162523)
J Am Chem Soc. 1971 Jan 13;93(1):217-24. (PMID: 5538858)
J Natl Cancer Inst. 1975 Sep;55(3):597-602. (PMID: 1159836)
Evid Based Complement Alternat Med. 2022 Nov 21;2022:6477132. (PMID: 36452140)
Semin Pediatr Surg. 2016 Oct;25(5):257-264. (PMID: 27955728)
WIREs Mech Dis. 2023 Jul-Aug;15(4):e1602. (PMID: 36750231)
Biomed Res Int. 2018 Dec 4;2018:1691428. (PMID: 30627539)
J Clin Invest. 2013 Sep;123(9):3706-8. (PMID: 23979156)
Phytochem Anal. 2004 Nov-Dec;15(6):345-54. (PMID: 15595449)
Genes Dev. 1999 Nov 15;13(22):2905-27. (PMID: 10579998)
Mol Cancer Ther. 2007 Nov;6(11):2921-7. (PMID: 18025276)
Plants (Basel). 2022 Oct 28;11(21):. (PMID: 36365350)
معلومات مُعتمدة: Fondecyt 1201395 ANID
فهرسة مساهمة: Keywords: Cannabinoids; Cannabinol; Caspases; Cell viability; Tetrahidrocannabinolic acid; Δ9-tetrahidrocannabinol
المشرفين على المادة: 0 (Plant Extracts)
EC 3.4.22.- (Caspase 3)
0 (Cannabinoids)
EC 3.4.22.- (Caspase 7)
تواريخ الأحداث: Date Created: 20240527 Date Completed: 20240528 Latest Revision: 20240603
رمز التحديث: 20240603
مُعرف محوري في PubMed: PMC11129430
DOI: 10.1186/s40659-024-00506-0
PMID: 38802872
قاعدة البيانات: MEDLINE
الوصف
تدمد:0717-6287
DOI:10.1186/s40659-024-00506-0