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Molecular and serological diagnosis of multiple bacterial zoonoses in febrile outpatients in Garissa County, north-eastern Kenya.

التفاصيل البيبلوغرافية
العنوان: Molecular and serological diagnosis of multiple bacterial zoonoses in febrile outpatients in Garissa County, north-eastern Kenya.
المؤلفون: Wainaina M; Animal and Human Health Program, International Livestock Research Institute, Nairobi, 00100, Kenya. markimwa@gmail.com.; Department of Veterinary Medicine, Freie Universität Berlin, 14163, Berlin, Germany. markimwa@gmail.com.; Department of Biological Safety, German Federal Institute for Risk Assessment, 12277, Berlin, Germany. markimwa@gmail.com., Lindahl JF; Animal and Human Health Program, International Livestock Research Institute, Nairobi, 00100, Kenya.; Department of Clinical Sciences, Swedish University of Agricultural Sciences, 75007, Uppsala, Sweden.; Department of Medical Biochemistry and Microbiology, Uppsala University, 75123, Uppsala, Sweden., Mayer-Scholl A; Department of Biological Safety, German Federal Institute for Risk Assessment, 12277, Berlin, Germany., Ufermann CM; Department of Biological Safety, German Federal Institute for Risk Assessment, 12277, Berlin, Germany., Domelevo Entfellner JB; Animal and Human Health Program, International Livestock Research Institute, Nairobi, 00100, Kenya., Roesler U; Institute for Animal Hygiene and Environmental Health, Freie Universität Berlin, 14163, Berlin, Germany., Roesel K; Animal and Human Health Program, International Livestock Research Institute, Nairobi, 00100, Kenya.; Department of Veterinary Medicine, Freie Universität Berlin, 14163, Berlin, Germany., Grace D; Animal and Human Health Program, International Livestock Research Institute, Nairobi, 00100, Kenya.; Food and Markets Department, Natural Resources Institute, University of Greenwich, London, ME130NQ, UK., Bett B; Animal and Human Health Program, International Livestock Research Institute, Nairobi, 00100, Kenya., Al Dahouk S; Department of Biological Safety, German Federal Institute for Risk Assessment, 12277, Berlin, Germany.; Department of Internal Medicine III, RWTH Aachen University Hospital, 52074, Aachen, Germany.; Department 1 - Infectious Diseases, Robert Koch Institute, 13353, Berlin, Germany.
المصدر: Scientific reports [Sci Rep] 2024 May 28; Vol. 14 (1), pp. 12263. Date of Electronic Publication: 2024 May 28.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Leptospirosis*/diagnosis , Leptospirosis*/epidemiology , Leptospirosis*/blood , Leptospirosis*/microbiology , Fever*/microbiology , Fever*/diagnosis , Fever*/epidemiology , Leptospira*/genetics , Leptospira*/isolation & purification , Leptospira*/immunology, Humans ; Kenya/epidemiology ; Adolescent ; Male ; Child ; Female ; Adult ; Child, Preschool ; Middle Aged ; Animals ; Young Adult ; Bacterial Zoonoses/diagnosis ; Bacterial Zoonoses/epidemiology ; Bacterial Zoonoses/microbiology ; Brucellosis/diagnosis ; Brucellosis/epidemiology ; Brucellosis/blood ; Brucellosis/microbiology ; Brucella/isolation & purification ; Brucella/immunology ; Brucella/genetics ; Outpatients ; Q Fever/diagnosis ; Q Fever/epidemiology ; Q Fever/microbiology ; Q Fever/blood ; Aged ; Serologic Tests ; Zoonoses/microbiology ; Zoonoses/diagnosis ; Zoonoses/epidemiology
مستخلص: Bacterial zoonoses are diseases caused by bacterial pathogens that can be naturally transmitted between humans and vertebrate animals. They are important causes of non-malarial fevers in Kenya, yet their epidemiology remains unclear. We investigated brucellosis, Q-fever and leptospirosis in the venous blood of 216 malaria-negative febrile patients recruited in two health centres (98 from Ijara and 118 from Sangailu health centres) in Garissa County in north-eastern Kenya. We determined exposure to the three zoonoses using serological (Rose Bengal test for Brucella spp., ELISA for C. burnetti and microscopic agglutination test for Leptospira spp.) and real-time PCR testing and identified risk factors for exposure. We also used non-targeted metagenomic sequencing on nine selected patients to assess the presence of other possible bacterial causes of non-malarial fevers. Considerable PCR positivity was found for Brucella (19.4%, 95% confidence intervals [CI] 14.2-25.5) and Leptospira spp. (1.7%, 95% CI 0.4-4.9), and high endpoint titres were observed against leptospiral serovar Grippotyphosa from the serological testing. Patients aged 5-17 years old had 4.02 (95% CI 1.18-13.70, p-value = 0.03) and 2.42 (95% CI 1.09-5.34, p-value = 0.03) times higher odds of infection with Brucella spp. and Coxiella burnetii than those of ages 35-80. Additionally, patients who sourced water from dams/springs, and other sources (protected wells, boreholes, bottled water, and water pans) had 2.39 (95% CI 1.22-4.68, p-value = 0.01) and 2.24 (1.15-4.35, p-value = 0.02) times higher odds of exposure to C. burnetii than those who used unprotected wells. Streptococcus and Moraxella spp. were determined using metagenomic sequencing. Brucellosis, leptospirosis, Streptococcus and Moraxella infections are potentially important causes of non-malarial fevers in Garissa. This knowledge can guide routine diagnosis, thus helping lower the disease burden and ensure better health outcomes, especially in younger populations.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 91693043 Deutscher Akademischer Austauschdienst
تواريخ الأحداث: Date Created: 20240528 Date Completed: 20240528 Latest Revision: 20240531
رمز التحديث: 20240531
مُعرف محوري في PubMed: PMC11133362
DOI: 10.1038/s41598-024-62714-8
PMID: 38806576
قاعدة البيانات: MEDLINE
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