دورية أكاديمية
Coix Sprouts Affect Triglyceride Metabolism in Huh7 Cells and High-Fat Diet-Induced Obese Mice.
| العنوان: | Coix Sprouts Affect Triglyceride Metabolism in Huh7 Cells and High-Fat Diet-Induced Obese Mice. |
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| المؤلفون: | Shin MR; Department of Herbology, College of Korean Medicine, Daegu Haany University, Daegu, Republic of Korea., Kim MJ; Department of Herbology, College of Korean Medicine, Daegu Haany University, Daegu, Republic of Korea.; Research Center for Herbal Convergence on Liver Disease, Daegu Haany University, Gyeongsan-si, Gyeongsangbuk-do, Republic of Korea., Lee JA; Department of Herbology, College of Korean Medicine, Daegu Haany University, Daegu, Republic of Korea., Lee ES; Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Eumseong, Republic of Korea., Park HJ; DHU Bio Convergence Testing Center, Daegu Haany University, Gyeongsan-si, Gyeongsangbuk-do, Republic of Korea., Roh SS; Department of Herbology, College of Korean Medicine, Daegu Haany University, Daegu, Republic of Korea. |
| المصدر: | Journal of medicinal food [J Med Food] 2024 Aug; Vol. 27 (8), pp. 728-739. Date of Electronic Publication: 2024 May 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 9812512 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7600 (Electronic) Linking ISSN: 1096620X NLM ISO Abbreviation: J Med Food Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c1998- |
| مواضيع طبية MeSH: | Diet, High-Fat*/adverse effects , Triglycerides*/metabolism , Mice, Inbred C57BL* , Obesity*/metabolism , Obesity*/drug therapy , Lipase*/metabolism , Lipase*/genetics , Mice, Obese* , Coix*, Animals ; Mice ; Male ; Humans ; Sterol Regulatory Element Binding Protein 1/metabolism ; Sterol Regulatory Element Binding Protein 1/genetics ; Plant Extracts/pharmacology ; Lipolysis/drug effects ; PPAR gamma/metabolism ; PPAR gamma/genetics ; Seedlings/metabolism ; Cell Line, Tumor ; Adipose Tissue/metabolism ; Adipose Tissue/drug effects |
| مستخلص: | Lipolysis is the hydrolysis of triglycerides (TGs), commonly known as fats. Intracellular lipolysis of TG is associated with adipose triglyceride lipase (ATGL), which provides fatty acids during times of metabolic need. The aim of this study was to determine whether Coix lacryma-jobi L. var. ma-yuen Stapf ( Coix ) sprouts (CS) can alleviate obesity through lipolysis. Overall, we investigated the potential of CS under in vitro and in vivo conditions and confirmed the underlying mechanisms. Huh7 cells were exposed to free fatty acids (FFAs), and C57BL/6J mice were fed a 60% high-fat diet. When FFA were introduced into Huh7 cells, the intracellular TG levels increased within the Huh7 cells. However, CS treatment significantly reduced intracellular TG levels. Furthermore, CS decreased the expression of Pparγ and Srebp1c mRNA and downregulated the mutant Pnpla3 (I148M) mRNA. Notably, CS significantly upregulated ATGL expression. CS treatment at a dose of 200 mg/kg/day resulted in a significant and dose-dependent decrease in body weight gain and epididymal adipose tissue weight. Specifically, the group treated with CS (200 mg/kg/day) exhibited a significant modulation of serum lipid biomarkers. In addition, CS ameliorated histological alterations in both the liver and adipose tissues. In summary, CS efficiently inhibited lipid accumulation through the activation of the lipolytic enzyme ATGL coupled with the suppression of enzymes involved in TG synthesis. Consequently, CS show promise as a potential anti-obesity agent. |
| فهرسة مساهمة: | Keywords: Coix lacryma-jobi L. var. ma-yuen Stapf; Coix sprouts; anti-obesity; high-fat diet; lipolysis |
| المشرفين على المادة: | 0 (Triglycerides) EC 3.1.1.3 (Lipase) 0 (Sterol Regulatory Element Binding Protein 1) 0 (Plant Extracts) 0 (PPAR gamma) |
| تواريخ الأحداث: | Date Created: 20240529 Date Completed: 20240816 Latest Revision: 20240816 |
| رمز التحديث: | 20240817 |
| DOI: | 10.1089/jmf.2023.k.0246 |
| PMID: | 38808469 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1557-7600 |
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| DOI: | 10.1089/jmf.2023.k.0246 |