دورية أكاديمية
Danshensu reduces neuronal excitability by enhancing potassium currents in bushy cells in the mouse cochlear nucleus.
| العنوان: | Danshensu reduces neuronal excitability by enhancing potassium currents in bushy cells in the mouse cochlear nucleus. |
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| المؤلفون: | Xu M; Department of Life Sciences.; Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes, Anhui University, Hefei., Wang L; Department of Otorhinolaryngology, ENT Institute, and NHC Key Laboratory of Hearing Medicine, Eye & ENT Hospital.; Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, and MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China., Li GL; Department of Otorhinolaryngology, ENT Institute, and NHC Key Laboratory of Hearing Medicine, Eye & ENT Hospital.; Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, and MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China., Tang ZQ; Department of Life Sciences.; Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes, Anhui University, Hefei. |
| المصدر: | Neuroreport [Neuroreport] 2024 Jul 01; Vol. 35 (10), pp. 638-647. Date of Electronic Publication: 2024 May 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Lippincott Williams & Wilkins Country of Publication: England NLM ID: 9100935 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1473-558X (Electronic) Linking ISSN: 09594965 NLM ISO Abbreviation: Neuroreport Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London, England : Lippincott Williams & Wilkins Original Publication: Oxford, UK : Rapid Communications of Oxford Ltd., [1990- |
| مواضيع طبية MeSH: | Neurons*/drug effects , Neurons*/physiology , Lactates*/pharmacology , Cochlear Nucleus*/drug effects , Cochlear Nucleus*/physiology, Animals ; Mice ; Patch-Clamp Techniques ; Action Potentials/drug effects ; Action Potentials/physiology ; Male ; Potassium Channels/drug effects ; Potassium Channels/metabolism ; Mice, Inbred C57BL |
| مستخلص: | Danshensu, also known as salvianic acid A, is a primary active compound extracted from a traditional Chinese herb Danshen (Salvia miltiorrhiza). While its antioxidative and neuroprotective effects are well-documented, the underlying mechanisms are poorly understood. In this study, we sought out to investigate if and how Danshensu modulates neuronal excitability and voltage-gated ionic currents in the central nervous system. We prepared brain slices of the mouse brainstem and performed patch-clamp recording in bushy cells in the anteroventral cochlear nucleus, with or without Danshensu incubation for 1 h. QX-314 was used internally to block Na+ current, while tetraethylammonium and 4-aminopyridine were used to isolate different subtypes of K+ current. We found that Danshensu of 100 μm decreased the input resistance of bushy cells by approximately 60% and shifted the voltage threshold of spiking positively by approximately 7 mV, resulting in significantly reduced excitability. Furthermore, we found this reduced excitability by Danshensu was caused by enhanced voltage-gated K+ currents in these neurons, including both low voltage-activated IK,A, by approximately 100%, and high voltage-activated IK,dr, by approximately 30%. Lastly, we found that the effect of Danshensu on K+ currents was dose-dependent in that no enhancement was found for Danshensu of 50 μm and Danshensu of 200 μm failed to cause significantly more enhancement on K+ currents when compared to that of 100 μm. We found that Danshensu reduced neuronal excitability in the central nervous system by enhancing voltage-gated K+ currents, providing mechanistic support for its neuroprotective effect widely seen in vivo. (Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.) |
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| المشرفين على المادة: | 4GF33A5PAJ (3,4-dihydroxyphenyllactic acid) 0 (Lactates) 0 (Potassium Channels) |
| تواريخ الأحداث: | Date Created: 20240530 Date Completed: 20240530 Latest Revision: 20240625 |
| رمز التحديث: | 20240626 |
| DOI: | 10.1097/WNR.0000000000002047 |
| PMID: | 38813908 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1473-558X |
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| DOI: | 10.1097/WNR.0000000000002047 |