دورية أكاديمية
أعرض في EDS

Two-Stage Mixed-Dye-Based Isothermal Amplification with Ribonuclease-Cleavable Enhanced Probes for Dual-Visualization Detection of SARS-CoV-2 Variants of Interest.

التفاصيل البيبلوغرافية
العنوان: Two-Stage Mixed-Dye-Based Isothermal Amplification with Ribonuclease-Cleavable Enhanced Probes for Dual-Visualization Detection of SARS-CoV-2 Variants of Interest.
المؤلفون: Ding X; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing, 210009, P. R. China., Wang Y; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing, 210009, P. R. China., Gui Y; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing, 210009, P. R. China., Yang C; Center of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing, 210009, P. R. China.
المصدر: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Aug; Vol. 11 (29), pp. e2401988. Date of Electronic Publication: 2024 Jun 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: WILEY-VCH Country of Publication: Germany NLM ID: 101664569 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2198-3844 (Electronic) Linking ISSN: 21983844 NLM ISO Abbreviation: Adv Sci (Weinh) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Weinheim : WILEY-VCH, [2014]-
مواضيع طبية MeSH: SARS-CoV-2*/genetics , Nucleic Acid Amplification Techniques*/methods , COVID-19*/virology , COVID-19*/diagnosis, Humans ; Molecular Diagnostic Techniques/methods ; Ribonucleases/metabolism ; RNA, Viral/genetics ; Sensitivity and Specificity ; Fluorescent Dyes/chemistry
مستخلص: Rapid and visual detection of SARS-CoV-2 variants is vital for timely assessment of variant transmission in resource-limited settings. Here, a closed-tube, two-stage, mixed-dye-based isothermal amplification method with ribonuclease-cleavable enhanced probes (REP), termed REP-TMAP, for dual-visualization detection of SARS-CoV-2 variants including JN.1, BA.2, BA.4/5, and Delta is introduced. The first stage of REP-TMAP is reverse transcription recombinase polymerase amplification and the second stage is dual-visualization detection synergistically mediated by the REP and the mixed dyes of cresol red and hydroxy naphthol blue. In REP-TMAP reaction, the color change under ambient light indicates SARS-CoV-2 infection, while the fluorescence change under blue light excitation specifies variant type. On detecting transcribed RNA of SARS-CoV-2 spike gene, this assay is rapid (within 40 min), highly sensitive (10-200 copies per reaction), and highly specific (identification of single-base mutations). Furthermore, the assay has been clinically validated to accurately detect JN.1, BA.2, and BA.4/5 variants from 102 human oropharyngeal swabs. The proposed assay therefore holds great potentials to provide a rapid, dual-visualization, sensitive, specific, point-of-care detection of SARS-CoV-2 variants and beyond.
(© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)
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معلومات مُعتمدة: 2022YFF1102600 National Key Research and Development Program of China; 82102498 National Natural Science Foundation of China; 82373629 National Natural Science Foundation of China; 2242023K5007 Fundamental Research Funds for the Central Universities; 4060692202/023 Medical Foundation of Southeast University; BE2023725 Social Development Research Program of Jiangsu Province Science and Technology Department
فهرسة مساهمة: Keywords: REP‐TMAP; SARS‐CoV‐2 variants of interest; dual‐visualization detection; nucleic acid amplification; ribonuclease‐cleavable enhanced probes
المشرفين على المادة: EC 3.1.- (Ribonucleases)
0 (RNA, Viral)
0 (Fluorescent Dyes)
SCR Protocol: LAMP assay
SCR Organism: SARS-CoV-2 variants
تواريخ الأحداث: Date Created: 20240603 Date Completed: 20240807 Latest Revision: 20240809
رمز التحديث: 20240809
مُعرف محوري في PubMed: PMC11304323
DOI: 10.1002/advs.202401988
PMID: 38829265
قاعدة البيانات: MEDLINE
الوصف
تدمد:2198-3844
DOI:10.1002/advs.202401988