دورية أكاديمية
أعرض في EDS

Assembly of the Tn7 targeting complex by a regulated stepwise process.

التفاصيل البيبلوغرافية
العنوان: Assembly of the Tn7 targeting complex by a regulated stepwise process.
المؤلفون: Shen Y; Department of Biochemistry, McGill University, Montreal, QC H3G 0B1, Canada; Centre de recherche en biologie structurale (CRBS), McGill University, Montreal, QC H3G 0B1, Canada., Krishnan SS; Department of Biochemistry, McGill University, Montreal, QC H3G 0B1, Canada; Centre de recherche en biologie structurale (CRBS), McGill University, Montreal, QC H3G 0B1, Canada., Petassi MT; Department of Microbiology, Cornell University, Ithaca, NY 14853, USA., Hancock MA; Centre de recherche en biologie structurale (CRBS), McGill University, Montreal, QC H3G 0B1, Canada; Department of Pharmacology and Therapeutics, McGill University, Montreal, QC H3G 1Y6, Canada., Peters JE; Department of Microbiology, Cornell University, Ithaca, NY 14853, USA., Guarné A; Department of Biochemistry, McGill University, Montreal, QC H3G 0B1, Canada; Centre de recherche en biologie structurale (CRBS), McGill University, Montreal, QC H3G 0B1, Canada. Electronic address: alba.guarne@mcgill.ca.
المصدر: Molecular cell [Mol Cell] 2024 Jun 20; Vol. 84 (12), pp. 2368-2381.e6. Date of Electronic Publication: 2024 Jun 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 9802571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4164 (Electronic) Linking ISSN: 10972765 NLM ISO Abbreviation: Mol Cell Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge Ma : Cell Press
Original Publication: Cambridge, Mass. : Cell Press, c1997-
مواضيع طبية MeSH: Cryoelectron Microscopy* , DNA Transposable Elements*/genetics , Adenosine Triphosphate*/metabolism , Transposases*/metabolism , Transposases*/genetics , Transposases*/chemistry , Adenosine Diphosphate*/metabolism, Protein Binding ; Bacterial Proteins/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/chemistry ; Models, Molecular ; Protein Multimerization ; Binding Sites
مستخلص: The Tn7 family of transposons is notable for its highly regulated integration mechanisms, including programmable RNA-guided transposition. The targeting pathways rely on dedicated target selection proteins from the TniQ family and the AAA+ adaptor TnsC to recruit and activate the transposase at specific target sites. Here, we report the cryoelectron microscopy (cryo-EM) structures of TnsC bound to the TniQ domain of TnsD from prototypical Tn7 and unveil key regulatory steps stemming from unique behaviors of ATP- versus ADP-bound TnsC. We show that TnsD recruits ADP-bound dimers of TnsC and acts as an exchange factor to release one protomer with exchange to ATP. This loading process explains how TnsC assembles a heptameric ring unidirectionally from the target site. This unique loading process results in functionally distinct TnsC protomers within the ring, providing a checkpoint for target immunity and explaining how insertions at programmed sites precisely occur in a specific orientation across Tn7 elements.
Competing Interests: Declaration of interests Cornell University has filed patent applications with J.E.P. and M.T.P. as inventors involving CRISPR-Cas systems associated with transposons that are not related to this work.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: R01 GM129118 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: AAA+ ATPase; DNA transposition; Tn7 transposon; TnsD/TniQ proteins; cryo-EM; nucleotide-exchange factor; protein-DNA interactions; target-site selection; transposition immunity
المشرفين على المادة: 0 (DNA Transposable Elements)
8L70Q75FXE (Adenosine Triphosphate)
EC 2.7.7.- (Transposases)
61D2G4IYVH (Adenosine Diphosphate)
0 (Bacterial Proteins)
تواريخ الأحداث: Date Created: 20240604 Date Completed: 20240621 Latest Revision: 20240831
رمز التحديث: 20240831
مُعرف محوري في PubMed: PMC11364213
DOI: 10.1016/j.molcel.2024.05.012
PMID: 38834067
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-4164
DOI:10.1016/j.molcel.2024.05.012