دورية أكاديمية
Structural characterization of Astragalus polysaccharide-D1 and its improvement of low-dose metformin effect by enriching Staphylococcus lentus.
| العنوان: | Structural characterization of Astragalus polysaccharide-D1 and its improvement of low-dose metformin effect by enriching Staphylococcus lentus. |
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| المؤلفون: | Long J; Beijing Institute of Clinical Pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, PR China., Li M; College of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, PR China., Yao C; Beijing Institute of Clinical Pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, PR China., Ma W; Beijing Institute of Clinical Pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, PR China., Liu H; College of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, PR China. Electronic address: hongtaoliu@hbtcm.edu.cn., Yan D; Beijing Institute of Clinical Pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, PR China. Electronic address: danyan@ccmu.edu.cn. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2024 Jun; Vol. 272 (Pt 1), pp. 132860. Date of Electronic Publication: 2024 Jun 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Astragalus Plant*/chemistry , Polysaccharides*/chemistry , Polysaccharides*/isolation & purification , Polysaccharides*/pharmacology , Staphylococcus*/drug effects , Staphylococcus*/metabolism , Metformin*/administration & dosage , Metformin*/pharmacology , Hypoglycemic Agents*/administration & dosage , Hypoglycemic Agents*/pharmacology , Carnitine*/metabolism , Gastrointestinal Microbiome*/drug effects , Diabetes Mellitus, Type 2*/drug therapy , Diabetes Mellitus, Type 2*/microbiology, Male ; Animals ; Mice ; Mice, Inbred C57BL ; Insulin Resistance |
| مستخلص: | To explore the adjuvant therapy drugs of low-dose metformin, one homogeneous polysaccharide named APS-D1 was purified from Astragalus membranaceus by DEAE-52 cellulose and Sephadex G-100 column chromatography. Its chemical structure was characterized by molecular weight distribution, monosaccharide composition, infrared spectrum, methylation analysis, and NMR. The results revealed that APS-D1 (7.36 kDa) consisted of glucose, galactose, and arabinose (97.51 %:1.56 %:0.93 %). It consisted of →4)-α-D-Glcp-(1→ residue backbone with →3)-β-D-Galp-(1→ residue and terminal-α/β-D-Glcp-(1→ side chains. APS-D1 could significantly improve inflammation (TNF-α, LPS, and IL-10) in vivo. Moreover, APS-D1 improved the curative effect of low-dose metformin without adverse events. APS-D1 combined with low-dose metformin regulated several gut bacteria, in which APS-D1 enriched Staphylococcus lentus to produce l-carnitine (one of 136 metabolites of S. lentus). S. lentus and l-carnitine could improve diabetes, and reduction of S. lentusl-carnitine production impaired diabetes improvement. The combination, S. lentus, and l-carnitine could promote fatty acid oxidation (CPT1) and inhibit gluconeogenesis (PCK and G6Pase). The results indicated that APS-D1 enhanced the curative effect of low-dose metformin to improve diabetes by enriching S. lentus, in which the effect of S. lentus was mediated by l-carnitine. Collectively, these findings support that low-dose metformin supplemented with APS-D1 may be a favorable therapeutic strategy for type 2 diabetes. Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Astragalus polysaccharide; Low-dose metformin; Staphylococcus lentus; Structural characterization |
| المشرفين على المادة: | 0 (Polysaccharides) 9100L32L2N (Metformin) 0 (Hypoglycemic Agents) S7UI8SM58A (Carnitine) |
| SCR Organism: | Staphylococcus lentus |
| تواريخ الأحداث: | Date Created: 20240604 Date Completed: 20240919 Latest Revision: 20240919 |
| رمز التحديث: | 20240920 |
| DOI: | 10.1016/j.ijbiomac.2024.132860 |
| PMID: | 38834117 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2024.132860 |