دورية أكاديمية
Specialized Tfh cell subsets driving type-1 and type-2 humoral responses in lymphoid tissue.
| العنوان: | Specialized Tfh cell subsets driving type-1 and type-2 humoral responses in lymphoid tissue. |
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| المؤلفون: | Kumar S; Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal.; Instituto Gulbenkian de Ciência, Oeiras, Portugal., Basto AP; Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal.; Instituto Gulbenkian de Ciência, Oeiras, Portugal.; CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Lisboa, Portugal.; Laboratório Associado para Ciência Animal e Veterinária (AL4AnimalS), Lisbon, Portugal., Ribeiro F; Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal.; Instituto Gulbenkian de Ciência, Oeiras, Portugal., Almeida SCP; Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal., Campos P; Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal., Peres C; Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, Lisboa, Portugal., Pulvirenti N; Istituto Nazionale di Genetica Molecolare, Milano, Italy., Al-Khalidi S; Institute of Cancer Sciences, University of Glasgow, Glasgow, UK.; Cancer Research UK Scotland Institute, Glasgow, UK., Kilbey A; Institute of Cancer Sciences, University of Glasgow, Glasgow, UK.; Cancer Research UK Scotland Institute, Glasgow, UK., Tosello J; Institut Curie, PSL Research University, INSERM U932, Paris, France., Piaggio E; Institut Curie, PSL Research University, INSERM U932, Paris, France., Russo M; Institute of Biomedical Sciences, Department of Immunology, University of Sao Paulo, Sao Paulo, Brazil., Gama-Carvalho M; BioISI - Biosystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, Lisboa, Portugal., Coffelt SB; Institute of Cancer Sciences, University of Glasgow, Glasgow, UK.; Cancer Research UK Scotland Institute, Glasgow, UK., Roberts EW; Institute of Cancer Sciences, University of Glasgow, Glasgow, UK.; Cancer Research UK Scotland Institute, Glasgow, UK., Geginat J; Istituto Nazionale di Genetica Molecolare, Milano, Italy.; Università degli studi di Milano, DISCCO, Milano, Italy., Florindo HF; Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, Lisboa, Portugal., Graca L; Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal. lgraca@medicina.ulisboa.pt.; Instituto Gulbenkian de Ciência, Oeiras, Portugal. lgraca@medicina.ulisboa.pt. |
| المصدر: | Cell discovery [Cell Discov] 2024 Jun 04; Vol. 10 (1), pp. 64. Date of Electronic Publication: 2024 Jun 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101661034 Publication Model: Electronic Cited Medium: Print ISSN: 2056-5968 (Print) Linking ISSN: 20565968 NLM ISO Abbreviation: Cell Discov Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : Nature Publishing Group, [2015]- |
| مستخلص: | Effective antibody responses are essential to generate protective humoral immunity. Different inflammatory signals polarize T cells towards appropriate effector phenotypes during an infection or immunization. Th1 and Th2 cells have been associated with the polarization of humoral responses. However, T follicular helper cells (Tfh) have a unique ability to access the B cell follicle and support the germinal center (GC) responses by providing B cell help. We investigated the specialization of Tfh cells induced under type-1 and type-2 conditions. We first studied homogenous Tfh cell populations generated by adoptively transferred TCR-transgenic T cells in mice immunized with type-1 and type-2 adjuvants. Using a machine learning approach, we established a gene expression signature that discriminates Tfh cells polarized towards type-1 and type-2 response, defined as Tfh1 and Tfh2 cells. The distinct signatures of Tfh1 and Tfh2 cells were validated against datasets of Tfh cells induced following lymphocytic choriomeningitis virus (LCMV) or helminth infection. We generated single-cell and spatial transcriptomics datasets to dissect the heterogeneity of Tfh cells and their localization under the two immunizing conditions. Besides a distinct specialization of GC Tfh cells under the two immunizations and in different regions of the lymph nodes, we found a population of Gzmk + Tfh cells specific for type-1 conditions. In human individuals, we could equally identify CMV-specific Tfh cells that expressed Gzmk. Our results show that Tfh cells acquire a specialized function under distinct types of immune responses and with particular properties within the B cell follicle and the GC. (© 2024. The Author(s).) |
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| معلومات مُعتمدة: | HR22-00741 "la Caixa" Foundation (Caixa Foundation); Enlght-ten 675395 EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) |
| تواريخ الأحداث: | Date Created: 20240604 Latest Revision: 20240607 |
| رمز التحديث: | 20240607 |
| مُعرف محوري في PubMed: | PMC11150427 |
| DOI: | 10.1038/s41421-024-00681-0 |
| PMID: | 38834551 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2056-5968 |
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| DOI: | 10.1038/s41421-024-00681-0 |