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Uniparental silencing of 5S rRNA genes in plant allopolyploids - insights from Cardamine (Brassicaceae).

التفاصيل البيبلوغرافية
العنوان: Uniparental silencing of 5S rRNA genes in plant allopolyploids - insights from Cardamine (Brassicaceae).
المؤلفون: Mandáková T; Central European Institute of Technology (CEITEC), Masaryk University, 625 00, Brno, Czech Republic.; Department of Experimental Biology, Faculty of Science, Masaryk University, 611 37, Brno, Czech Republic., Krumpolcová A; Department of Experimental Biology, Faculty of Science, Masaryk University, 611 37, Brno, Czech Republic.; Department of Molecular Epigenetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, 612 00, Brno, Czech Republic., Matyášek R; Department of Molecular Epigenetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, 612 00, Brno, Czech Republic., Volkov R; Department of Molecular Genetics and Biotechnology, Yuriy Fedkovych Chernivtsi National University, 58012, Chernivtsi, Ukraine., Lysak MA; Central European Institute of Technology (CEITEC), Masaryk University, 625 00, Brno, Czech Republic.; Faculty of Science, National Centre for Biomolecular Research, Masaryk University, 625 00, Brno, Czech Republic., Kovařík A; Department of Molecular Epigenetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, 612 00, Brno, Czech Republic.
المصدر: The Plant journal : for cell and molecular biology [Plant J] 2024 Aug; Vol. 119 (3), pp. 1313-1326. Date of Electronic Publication: 2024 Jun 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology Country of Publication: England NLM ID: 9207397 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-313X (Electronic) Linking ISSN: 09607412 NLM ISO Abbreviation: Plant J Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford : Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology, c1991-
مواضيع طبية MeSH: RNA, Ribosomal, 5S*/genetics , Polyploidy* , Cardamine*/genetics , Gene Silencing* , Phylogeny*, Genome, Plant/genetics ; DNA, Ribosomal/genetics ; In Situ Hybridization, Fluorescence ; Gene Expression Regulation, Plant
مستخلص: While the phenomenon of uniparental silencing of 35S rDNA in interspecific hybrids and allopolyploids is well documented, there is a notable absence of information regarding whether such silencing extends to the 5S RNA component of ribosomes. To address this gap in knowledge, we analyzed the 5S and 35S rDNA expression in Cardamine (Brassicaceae) allopolyploids, namely C. × insueta (2n = 3x = 24, genome composition RRA), C. flexuosa (2n = 4x = 32, AAHH), and C. scutata (2n = 4x = 32, PPAA) which share a common diploid ancestor (AA). We employed high-throughput sequencing of transcriptomes and genomes and phylogenetic analyses of 5S rRNA variants. The genomic organization of rDNA was further scrutinized through clustering and fluorescence in situ hybridization. In the C. × insueta allotriploid, we observed uniparental dominant expression of 5S and 35S rDNA loci. In the C. flexuosa and C. scutata allotetraploids, the expression pattern differed, with the 35S rDNA being expressed from the A subgenome, whereas the 5S rDNA was expressed from the partner subgenome. Both C. flexuosa and C. scutata but not C. × insueta showed copy and locus number changes. We conclude that in stabilized allopolyploids, transcription of ribosomal RNA components occurs from different subgenomes. This phenomenon appears to result in the formation of chimeric ribosomes comprising rRNA molecules derived from distinct parental origins. We speculate that the interplay of epigenetic silencing and rDNA rearrangements introduces an additional layer of variation in multimolecule ribosomal complexes, potentially contributing to the evolutionary success of allopolyploids.
(© 2024 The Author(s). The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)
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معلومات مُعتمدة: 20-28029S Grantová Agentura České Republiky; 22-16826S Grantová Agentura České Republiky; LM2018131 Czech Ministry of Education; MUNI/R/1268/2022 Masaryk University Grant Agency; CZ.02.01.01/00/22_008/0004581 ERDF Programme Johannes Amos Comenius
فهرسة مساهمة: Keywords: Cardamine; 35S rDNA; 5S rDNA; chromosome evolution; gene silencing; polyploidy; rRNA genes
المشرفين على المادة: 0 (RNA, Ribosomal, 5S)
0 (DNA, Ribosomal)
تواريخ الأحداث: Date Created: 20240605 Date Completed: 20240731 Latest Revision: 20240731
رمز التحديث: 20240731
DOI: 10.1111/tpj.16850
PMID: 38838061
قاعدة البيانات: MEDLINE
الوصف
تدمد:1365-313X
DOI:10.1111/tpj.16850