دورية أكاديمية
Occurrence, genetic characterization, and antibiotic susceptibility of Cronobacter spp. isolated from low water activity functional foods in Brazil.
| العنوان: | Occurrence, genetic characterization, and antibiotic susceptibility of Cronobacter spp. isolated from low water activity functional foods in Brazil. |
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| المؤلفون: | Cechin CDF; Universidade Estadual de Campinas (UNICAMP), Faculdade de Engenharia de Alimentos, Departamento de Ciência de Alimentos e Nutrição, Campinas, São Paulo, Brazil. Electronic address: carinecechin@hotmail.com., Carvalho GG; Universidade Estadual de Campinas (UNICAMP), Faculdade de Engenharia de Alimentos, Departamento de Ciência de Alimentos e Nutrição, Campinas, São Paulo, Brazil; Universidade Estadual de Campinas (UNICAMP), Instituto de Biologia, Departamento de Genética, Evolução, Microbiologia e Imunologia, Campinas, São Paulo, Brazil. Electronic address: gabriela.guimaraescarvalho@gmail.com., Kabuki DY; Universidade Estadual de Campinas (UNICAMP), Faculdade de Engenharia de Alimentos, Departamento de Ciência de Alimentos e Nutrição, Campinas, São Paulo, Brazil. Electronic address: kabuki@unicamp.br. |
| المصدر: | Food microbiology [Food Microbiol] 2024 Sep; Vol. 122, pp. 104570. Date of Electronic Publication: 2024 May 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 8601127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9998 (Electronic) Linking ISSN: 07400020 NLM ISO Abbreviation: Food Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Elsevier Original Publication: London ; Orlando : Academic Press, c1984- |
| مواضيع طبية MeSH: | Cronobacter*/genetics , Cronobacter*/drug effects , Cronobacter*/isolation & purification , Cronobacter*/classification , Anti-Bacterial Agents*/pharmacology , Microbial Sensitivity Tests* , Functional Food*, Brazil ; Food Microbiology ; Virulence Factors/genetics ; Bacterial Proteins/genetics ; Food Contamination/analysis ; Water ; Drug Resistance, Bacterial/genetics |
| مستخلص: | Cronobacter spp. are bacterial pathogens isolated from a wide variety of foods. This study aims at evaluating the occurrence of Cronobacter spp. in low water activity functional food samples, detect the presence of virulence genes, and determine the antibiotic susceptibility of strains. From 105 samples, 38 (36.2%) were contaminated with Cronobacter spp. The species identified by polymerase chain reaction (PCR) and sequencing analyses (rpoB and fusA genes, respectively) were C. sakazakii (60.3%), C. dublinensis (25.4%), C. turincensis (9.5%), and C. malonaticus (4.8%). Nineteen fusA alleles were identified, including four new alleles. The virulence genes were identified by PCR and all isolates were positive for ompX and sodA genes, 60.3% to cpa gene, and 58.7% to hly gene. Using the disk diffusion method, antibiotic susceptibility to twelve antibiotics was assessed twice, separated by a 19-month period. In the first test, the isolates showed diverse antibiotic susceptibility profiles, with nineteen isolates (30.2%) being multi-drug resistant (resistant to three or more antibiotic classes), in the second, the isolates were susceptible to all antibiotics. Cronobacter spp. in functional foods demonstrates the need for continued investigation of this pathogen in foods, and further research is needed to clarify the loss of resistance of Cronobacter strains. Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cronobacter; Functional foods; Loss antibiotic resistance; Multidrug-resistant strains; New fusA alleles; Virulence |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Virulence Factors) 0 (Bacterial Proteins) 059QF0KO0R (Water) |
| تواريخ الأحداث: | Date Created: 20240605 Date Completed: 20240605 Latest Revision: 20240605 |
| رمز التحديث: | 20240606 |
| DOI: | 10.1016/j.fm.2024.104570 |
| PMID: | 38839229 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-9998 |
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| DOI: | 10.1016/j.fm.2024.104570 |