دورية أكاديمية
أعرض في EDS

Single-cell transcriptomic atlas reveals increased regeneration in diseased human inner ear balance organs.

التفاصيل البيبلوغرافية
العنوان: Single-cell transcriptomic atlas reveals increased regeneration in diseased human inner ear balance organs.
المؤلفون: Wang T; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.; Department of Otolaryngology - Head and Neck Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province, 410011, PR China., Ling AH; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.; Department of Otolaryngology - Head and Neck Surgery, Epithelial Biology Center, Vanderbilt University Medical Center, Nashville, TN, 37232, USA., Billings SE; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Hosseini DK; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Vaisbuch Y; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Kim GS; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Atkinson PJ; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Sayyid ZN; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Aaron KA; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Wagh D; Stanford Genomics Facility, Stanford University School of Medicine, Stanford, CA, 94305, USA., Pham N; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Scheibinger M; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Zhou R; Department of Otolaryngology - Head and Neck Surgery, Epithelial Biology Center, Vanderbilt University Medical Center, Nashville, TN, 37232, USA., Ishiyama A; Department of Head and Neck Surgery, University of California Los Angeles, Los Angeles, CA, 90095, USA., Moore LS; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Maria PS; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Blevins NH; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Jackler RK; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Alyono JC; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Kveton J; Department of Surgery, Yale University School of Medicine, New Haven, CT, 06510, USA., Navaratnam D; Department of Surgery, Yale University School of Medicine, New Haven, CT, 06510, USA.; Department of Neurology, Yale University School of Medicine, New Haven, CT, 06510, USA., Heller S; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Lopez IA; Department of Head and Neck Surgery, University of California Los Angeles, Los Angeles, CA, 90095, USA., Grillet N; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA., Jan TA; Department of Otolaryngology - Head and Neck Surgery, Epithelial Biology Center, Vanderbilt University Medical Center, Nashville, TN, 37232, USA. taha.a.jan@vumc.org., Cheng AG; Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA. aglcheng@stanford.edu.
المصدر: Nature communications [Nat Commun] 2024 Jun 06; Vol. 15 (1), pp. 4833. Date of Electronic Publication: 2024 Jun 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Transcriptome* , Single-Cell Analysis* , Regeneration*/genetics, Humans ; Animals ; Mice ; Saccule and Utricle/metabolism ; Saccule and Utricle/cytology ; Neuroma, Acoustic/genetics ; Neuroma, Acoustic/metabolism ; Neuroma, Acoustic/pathology ; Ear, Inner/metabolism ; Ear, Inner/cytology ; Insulin-Like Growth Factor I/metabolism ; Insulin-Like Growth Factor I/genetics ; Male ; Hair Cells, Vestibular/metabolism ; Female ; Gene Expression Profiling
مستخلص: Mammalian inner ear hair cell loss leads to permanent hearing and balance dysfunction. In contrast to the cochlea, vestibular hair cells of the murine utricle have some regenerative capacity. Whether human utricular hair cells regenerate in vivo remains unknown. Here we procured live, mature utricles from organ donors and vestibular schwannoma patients, and present a validated single-cell transcriptomic atlas at unprecedented resolution. We describe markers of 13 sensory and non-sensory cell types, with partial overlap and correlation between transcriptomes of human and mouse hair cells and supporting cells. We further uncover transcriptomes unique to hair cell precursors, which are unexpectedly 14-fold more abundant in vestibular schwannoma utricles, demonstrating the existence of ongoing regeneration in humans. Lastly, supporting cell-to-hair cell trajectory analysis revealed 5 distinct patterns of dynamic gene expression and associated pathways, including Wnt and IGF-1 signaling. Our dataset constitutes a foundational resource, accessible via a web-based interface, serving to advance knowledge of the normal and diseased human inner ear.
(© 2024. The Author(s).)
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معلومات مُعتمدة: R01 AG081608 United States AG NIA NIH HHS; R01 DC016318 United States DC NIDCD NIH HHS; K08 DC019683 United States DC NIDCD NIH HHS; T32 DC015209 United States DC NIDCD NIH HHS; U24 DC015910 United States DC NIDCD NIH HHS; UL1 TR001085 United States TR NCATS NIH HHS; R01 DC021110 United States DC NIDCD NIH HHS; S10 OD018220 United States OD NIH HHS; R01 DC020879 United States DC NIDCD NIH HHS; R01 DC016409 United States DC NIDCD NIH HHS; F30 DC015698 United States DC NIDCD NIH HHS; U24 DC020857 United States DC NIDCD NIH HHS; R21 DC015879 United States DC NIDCD NIH HHS; R21 DC019457 United States DC NIDCD NIH HHS; S10 OD021763 United States OD NIH HHS; R01 DC016919 United States DC NIDCD NIH HHS; R01 DC013910 United States DC NIDCD NIH HHS; K24 DC020986 United States DC NIDCD NIH HHS
المشرفين على المادة: 67763-96-6 (Insulin-Like Growth Factor I)
تواريخ الأحداث: Date Created: 20240606 Date Completed: 20240606 Latest Revision: 20240610
رمز التحديث: 20240610
مُعرف محوري في PubMed: PMC11156867
DOI: 10.1038/s41467-024-48491-y
PMID: 38844821
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-024-48491-y