دورية أكاديمية
Whole genome sequencing analysis of seven unknown resistance mechanisms of carbapenem-resistant Klebsiella pneumoniae strains resistance to ceftazidime-avibactam.
| العنوان: | Whole genome sequencing analysis of seven unknown resistance mechanisms of carbapenem-resistant Klebsiella pneumoniae strains resistance to ceftazidime-avibactam. |
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| المؤلفون: | Chen Y; Clinical Laboratory, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, Fujian 350525, China., Huang J; Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou 350001, China., Chen H; Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou 350001, China., Xiao H; Medical Technology, First Clinical Medical College, Fujian University of Traditional Chinese Medicine, Fuzhou 350100, China., Shen X; Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou 350001, China., Chen Q; Clinical Laboratory, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, Fujian 350525, China., Zhang Z; Clinical Laboratory, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, Fujian 350525, China., Huang Y; Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou 350001, China., Wu S; Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou 350001, China., Chen D; Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou 350001, China. |
| المصدر: | Journal of applied microbiology [J Appl Microbiol] 2024 Jun 03; Vol. 135 (6). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2022- : Oxford : Oxford University Press Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997- |
| مواضيع طبية MeSH: | Ceftazidime*/pharmacology , Klebsiella pneumoniae*/genetics , Klebsiella pneumoniae*/drug effects , Azabicyclo Compounds*/pharmacology , Whole Genome Sequencing* , Drug Combinations* , Anti-Bacterial Agents*/pharmacology , Microbial Sensitivity Tests*, Genome, Bacterial ; Drug Resistance, Multiple, Bacterial/genetics ; Humans ; Carbapenem-Resistant Enterobacteriaceae/genetics ; Carbapenem-Resistant Enterobacteriaceae/drug effects ; Klebsiella Infections/microbiology ; Carbapenems/pharmacology ; Virulence Factors/genetics |
| مستخلص: | Aims: To investigate alternative resistance mechanisms among seven ceftazidime-avibactam (CZA)-resistant carbapenem-resistant Klebsiella pneumoniae (CRKP) strains lacking common antimicrobial resistance genes (ARGs) using whole genome sequencing. Methods and Results: ARG and virulence factors (VFs) were screened using the ARG database CARD and the VF database, respectively, and identified using genomic annotation data with BLAST+. Six strains were ST11 sequence types (STs), and one was ST2123. ST11 strains harbored more ARGs than the ST2123 strains. All seven strains carried multiple ARGs with efflux-mediated antibiotic resistance, including oqxA, oqxB, tet (A), qacEdltal, CRP, H-NS, Kpn-E, F, G, H, acrA, LptD, acrB, acrD, cpxA, mdtB, and mdtC. These efflux-mediated ARGs were identified in most strains and even all strains. Whole genome sequencing revealed that the ST11 strain carried multiple potential prophages, genomic islands, and integrative and conjugative elements, while the ST2123 strain carried an independent potential prophages and a genomic island. Conclusions: Whole genome sequencing analysis revealed that these seven CZA-resistant CRKP strains lacking common ARGs exhibited efflux-mediated antibiotic resistance-associated ARGs. The main mechanism by which CRKP resists CZA is antibiotic inactivation. Except for tet (A), no ARGs and validation experiments related to efflux were found. This study's results provide a new possibility for the resistance mechanism of CRKP to CZA, and we will verify this conclusion through experiments in the future. (© The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.) |
| معلومات مُعتمدة: | 2020J05258 Fujian Natural Science Foundation; 2020GGA006 Health Science and Technology Program of Fujian province |
| فهرسة مساهمة: | Keywords: Klebsiella pneumoniae; antimicrobial resistance genes; carbapenem-resistance; ceftazidime–avibactam; whole genome sequencing |
| المشرفين على المادة: | 9M416Z9QNR (Ceftazidime) 0 (avibactam, ceftazidime drug combination) 0 (Azabicyclo Compounds) 0 (Drug Combinations) 0 (Anti-Bacterial Agents) 0 (Carbapenems) 0 (Virulence Factors) |
| تواريخ الأحداث: | Date Created: 20240607 Date Completed: 20240614 Latest Revision: 20240614 |
| رمز التحديث: | 20240615 |
| DOI: | 10.1093/jambio/lxae135 |
| PMID: | 38849309 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1365-2672 |
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| DOI: | 10.1093/jambio/lxae135 |