دورية أكاديمية
أعرض في EDS

Varying the position of phospholipid acyl chain unsaturation modulates hopanoid and sterol ordering.

التفاصيل البيبلوغرافية
العنوان: Varying the position of phospholipid acyl chain unsaturation modulates hopanoid and sterol ordering.
المؤلفون: Nguyen HNA; Technische Universität Dresden, B CUBE Center for Molecular Bioengineering, Dresden, Germany., Sharp L; Department of Physics and Astronomy, University of Delaware, Newark, Delaware; College of Arts and Sciences, Fairfield University, Fairfield, Connecticut., Lyman E; Department of Physics and Astronomy, University of Delaware, Newark, Delaware; Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware., Saenz JP; Technische Universität Dresden, B CUBE Center for Molecular Bioengineering, Dresden, Germany; Medical Faculty, Technische Universität Dresden, Dresden, Germany. Electronic address: james.saenz@tu-dresden.de.
المصدر: Biophysical journal [Biophys J] 2024 Jul 02; Vol. 123 (13), pp. 1896-1902. Date of Electronic Publication: 2024 Jun 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 0370626 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1542-0086 (Electronic) Linking ISSN: 00063495 NLM ISO Abbreviation: Biophys J Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge, MA : Cell Press
Original Publication: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society.
مواضيع طبية MeSH: Sterols*/chemistry , Sterols*/metabolism , Phospholipids*/chemistry , Phospholipids*/metabolism, Triterpenes/chemistry ; Triterpenes/metabolism ; Cholesterol/chemistry ; Cholesterol/metabolism
مستخلص: The cell membrane must balance mechanical stability with fluidity to function as both a barrier and an organizational platform. Key to this balance is the ordering of hydrocarbon chains and the packing of lipids. Many eukaryotes synthesize sterols, which are uniquely capable of modulating the lipid order to decouple membrane stability from fluidity. Ancient sterol analogs known as hopanoids are found in many bacteria and proposed as ancestral ordering lipids. The juxtaposition of sterols and hopanoids in extant organisms prompts us to ask why both pathways persist, especially in light of their convergent ability to order lipids. In this work, simulations, monolayer experiments, and cellular assays show that hopanoids and sterols order unsaturated phospholipids differently based on the position of double bonds in the phospholipid acyl chain. We find that cholesterol and diplopterol's methyl group distributions lead to distinct effects on unsaturated lipids. In Mesoplasma florum, diplopterol's constrained ordering capacity reduces membrane resistance to osmotic stress, unlike cholesterol. These findings suggest that cholesterol's broader lipid-ordering ability may have facilitated the exploration of a more diverse lipidomic landscape in eukaryotic membranes.
Competing Interests: Declaration of interests The authors declared no competing interests.
(Copyright © 2024 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
التعليقات: Update of: bioRxiv. 2024 Feb 09:2023.09.06.556521. doi: 10.1101/2023.09.06.556521. (PMID: 38370701)
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معلومات مُعتمدة: R01 GM120351 United States GM NIGMS NIH HHS
المشرفين على المادة: 0 (Sterols)
0 (Phospholipids)
0 (Triterpenes)
97C5T2UQ7J (Cholesterol)
تواريخ الأحداث: Date Created: 20240608 Date Completed: 20240703 Latest Revision: 20240810
رمز التحديث: 20240812
مُعرف محوري في PubMed: PMC11267422
DOI: 10.1016/j.bpj.2024.06.002
PMID: 38850024
قاعدة البيانات: MEDLINE
الوصف
تدمد:1542-0086
DOI:10.1016/j.bpj.2024.06.002