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Plakins are involved in the regulation of centrosome position in polarized epithelial cells.

التفاصيل البيبلوغرافية
العنوان: Plakins are involved in the regulation of centrosome position in polarized epithelial cells.
المؤلفون: Geay J; Université de Paris, CEA/INSERM/AP-HP, Institut de Recherche Saint Louis, UMR976, HIPI, CytoMorpho Lab, Hopital Saint Louis, Paris, France., Margaron Y; Université Grenoble-Alpes, CEA/INRA/CNRS, Interdisciplinary Research Institute of Grenoble, UMR5168, LPCV, CytoMorpho Lab, Grenoble, France., Gentien D; Université PSL, Department of Translational Research, Institut Curie, Genomics Platform, Paris, France., Reyal F; Université Paris Cité, Université PSL, INSERM U932, Breast Gynecological and Reconstructive Surgery, Institut Curie, Paris, France., Puisieux A; Université Claude Bernard Lyon 1, Cancer Research Center of Lyon, INSERM 1052, CNRS 5286, Centre Léon Bérard, Lyon, France.; Université PSL, Institut Curie, Université Versailles Saint-Quentin, CNRS UMR 3666, INSERM U1143, Paris, France., Blanchoin L; Université de Paris, CEA/INSERM/AP-HP, Institut de Recherche Saint Louis, UMR976, HIPI, CytoMorpho Lab, Hopital Saint Louis, Paris, France.; Université Grenoble-Alpes, CEA/INRA/CNRS, Interdisciplinary Research Institute of Grenoble, UMR5168, LPCV, CytoMorpho Lab, Grenoble, France., Guyon L; Université Grenoble Alpes, CEA/INSERM, Interdisciplinary Research Institute of Grenoble, BioSanté UMR_S 1292, Grenoble, France., Théry M; Université de Paris, CEA/INSERM/AP-HP, Institut de Recherche Saint Louis, UMR976, HIPI, CytoMorpho Lab, Hopital Saint Louis, Paris, France.; Université Grenoble-Alpes, CEA/INRA/CNRS, Interdisciplinary Research Institute of Grenoble, UMR5168, LPCV, CytoMorpho Lab, Grenoble, France.
المصدر: Biology of the cell [Biol Cell] 2024 Jul; Vol. 116 (7), pp. e2400048. Date of Electronic Publication: 2024 Jun 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 8108529 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1768-322X (Electronic) Linking ISSN: 02484900 NLM ISO Abbreviation: Biol Cell Subsets: MEDLINE
أسماء مطبوعة: Publication: 2012- : Chichester : Wiley-Blackwell
Original Publication: Ivry sur Seine France : Publié par la Société française de microscopie électronique avec le concours du Centre national de la recherche scientifique et de l'Institute national de la santé et de la recherche médicale, [1981-
مواضيع طبية MeSH: Centrosome*/metabolism , Epithelial Cells*/metabolism , Cell Polarity* , Epithelial-Mesenchymal Transition* , Plakins*/metabolism , Plakins*/genetics, Humans ; Female ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Breast Neoplasms/genetics ; Cell Line, Tumor ; Microtubules/metabolism
مستخلص: Background Information: The control of epithelial cell polarity is key to their function. Its dysregulation is a major cause of tissue transformation. In polarized epithelial cells,the centrosome is off-centred toward the apical pole. This asymmetry determines the main orientation of the microtubule network and intra-cellular traffic. However, the mechanism regulating centrosome positioning at the apical pole of polarized epithelial cells is still poorly undertood.
Results: In this study we used transcriptomic data from breast cancer cells to identify molecular changes associated with the different stages of tumour transformation. We correlated these changes with variations in centrosome position or with cell progression along the epithelial-to-mesenchymal transition (EMT), a process that involves centrosome repositioning. We found that low levels of epiplakin, desmoplakin and periplakin correlated with centrosome mispositioning in cells that had progressed through EMT or tissue transformation. We further tested the causal role of these plakins in the regulation of centrosome position by knocking down their expression in a non-tumorigenic breast epithelial cell line (MCF10A). The downregulation of periplakin reduced the length of intercellular junction, which was not affected by the downregulation of epiplakin or desmoplakin. However, down-regulating any of them disrupted centrosome polarisation towards the junction without affecting microtubule stability.
Conclusions: Altogether, these results demonstrated that epiplakin, desmoplakin and periplakin are involved in the maintenance of the peripheral position of the centrosome close to inter-cellular junctions. They also revealed that these plakins are downregulated during EMT and breast cancer progression, which are both associated with centrosome mispositioning.
Significance: These results revealed that the down-regulation of plakins and the consequential centrosome mispositioning are key signatures of disorganised cytoskeleton networks, inter-cellular junction weakening, shape deregulation and the loss of polarity in breast cancer cells. These metrics could further be used as a new readouts for early phases of tumoral development.
(© 2024 The Author(s). Biology of the Cell published by Wiley‐VCH GmbH on behalf of Société Française des Microscopies and Société de Biologie Cellulaire de France.)
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معلومات مُعتمدة: 771599 International ERC_ European Research Council; 780458 International ERC_ European Research Council
المشرفين على المادة: 0 (Plakins)
تواريخ الأحداث: Date Created: 20240608 Date Completed: 20240712 Latest Revision: 20240712
رمز التحديث: 20240712
DOI: 10.1111/boc.202400048
PMID: 38850178
قاعدة البيانات: MEDLINE
الوصف
تدمد:1768-322X
DOI:10.1111/boc.202400048