دورية أكاديمية
Effects of mesenchymal stem cells versus curcumin on sonic hedgehog signaling in experimental model of Hepatocellular Carcinoma.
| العنوان: | Effects of mesenchymal stem cells versus curcumin on sonic hedgehog signaling in experimental model of Hepatocellular Carcinoma. |
|---|---|
| المؤلفون: | Abdel-Tawab MS; Medical Biochemistry Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt. Drmarwasayed2016@gmail.com., Fouad H; Medical Biochemistry Department, Faculty of Medicine, Cairo University, POB 12613, Cairo, Egypt.; Faculty of Medicine, Galala University, POB 43711, Attaka, Suez Governorate, Egypt., Sedeak AY; Anatomy and Embryology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt., Doudar NA; Clinical and Chemical Pathology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt., Rateb EE; Physiology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt., Faruk E; Department of Anatomy, Faculty of Medicine, Umm Al-Qura University, Mecca, Saudi Arabia.; Department of Histology and Cytology, Faculty of Medicine, Benha University, Benha, Egypt., Reyad HR; Medical Biochemistry Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt. |
| المصدر: | Molecular biology reports [Mol Biol Rep] 2024 Jun 14; Vol. 51 (1), pp. 740. Date of Electronic Publication: 2024 Jun 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Dordrecht, Boston, Reidel. |
| مواضيع طبية MeSH: | Hedgehog Proteins*/metabolism , Hedgehog Proteins*/genetics , Curcumin*/pharmacology , Carcinoma, Hepatocellular*/genetics , Carcinoma, Hepatocellular*/metabolism , Carcinoma, Hepatocellular*/pathology , Signal Transduction*/drug effects , Mesenchymal Stem Cells*/metabolism , Mesenchymal Stem Cells*/drug effects , Liver Neoplasms*/genetics , Liver Neoplasms*/metabolism , Liver Neoplasms*/pathology, Animals ; Rats ; Mesenchymal Stem Cell Transplantation/methods ; Male ; Disease Models, Animal ; Patched-1 Receptor/genetics ; Patched-1 Receptor/metabolism ; Zinc Finger Protein GLI1/metabolism ; Zinc Finger Protein GLI1/genetics ; Gene Expression Regulation, Neoplastic/drug effects ; Liver/metabolism ; Liver/pathology ; Liver/drug effects |
| مستخلص: | Background: Sonic Hedgehog (SHH) is a fundamental signaling pathway that controls tissue reconstruction, stem cell biology, and differentiation and has a role in gut tissue homeostasis and development. Dysregulation of SHH leads to the development of HCC. Methods, and Results: The present study was conducted to compare the effects of mesenchymal stem cells (MSCs) and curcumin on SHH molecular targets in an experimental model of HCC in rats. One hundred rats were divided equally into the following groups: control group, HCC group, HCC group received MSCs, HCC group received curcumin, and HCC group received MSCs and curcumin. Histopathological examinations were performed, and gene expression of SHH signaling target genes (SHH, PTCH1, SMOH, and GLI1) was assessed by real-time PCR in rat liver tissue. Results showed that SHH target genes were significantly upregulated in HCC-untreated rat groups and in MSC-treated groups, with no significant difference between them. Administration of curcumin with or without combined administration of MSCs led to a significant down-regulation of SHH target genes, with no significant differences between both groups. As regards the histopathological examination of liver tissues, both curcumin and MSCs, either through separate use or their combined use, led to a significant restoration of normal liver pathology. Conclusions: In conclusion, SHH signaling is upregulated in the HCC experimental model. MSCs do not inhibit the upregulated SHH target genes in HCC. Curcumin use with or without MSCs administration led to a significant down-regulation of SHH signaling in HCC and a significant restoration of normal liver pathology. (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.) |
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(PMID: 10.1007/s12015-014-9515-2) |
| فهرسة مساهمة: | Keywords: And GLI1; PTCH1; SHH; SMOH; Curcumin; HCC; MSCs; Sonic hedgehog |
| المشرفين على المادة: | 0 (Hedgehog Proteins) IT942ZTH98 (Curcumin) 0 (Shh protein, rat) 0 (Patched-1 Receptor) 0 (Zinc Finger Protein GLI1) 0 (Gli1 protein, rat) |
| تواريخ الأحداث: | Date Created: 20240614 Date Completed: 20240614 Latest Revision: 20240627 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1007/s11033-024-09613-3 |
| PMID: | 38874802 |
| قاعدة البيانات: | MEDLINE |
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